Cargando…
Arabidopsis : a practical approach /
"Arabidopsis has long been acknowledged as the ideal molecular genetic tool for the analysis of development in higher plants. This book covers all the key techniques for using Arabidopsis as an experimental system. This fully up-to-date and comprehensive laboratory manual is an essential source...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford ; New York :
Oxford University Press,
2000.
|
| Colección: | Practical approach series ;
223. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
| LEADER | 00000cam a2200000 a 4500 | ||
|---|---|---|---|
| 001 | EBSCO_ocm53982369 | ||
| 003 | OCoLC | ||
| 005 | 20231017213018.0 | ||
| 006 | m o d | ||
| 007 | cr cnu---unuuu | ||
| 008 | 040106s2000 enka ob 001 0 eng d | ||
| 040 | |a N$T |b eng |e pn |c N$T |d OCLCQ |d YDXCP |d OCLCQ |d IDEBK |d OCLCQ |d OCLCO |d OCLCQ |d INTCL |d TOA |d AGLDB |d OCLCQ |d OCLCO |d OCLCA |d INARC |d OCLCQ |d OCLCF |d VNS |d VTS |d EBLCP |d S9I |d G3B |d STF |d OCLCQ |d OCLCO |d OCLCQ |d YDX |d OCLCO | ||
| 015 | |a GB99V0916 |2 bnb | ||
| 015 | |a GBA005029 |2 bnb | ||
| 016 | 7 | |a 101296426 |2 DNLM | |
| 016 | 7 | |a 008428880 |2 Uk | |
| 019 | |a 666955394 |a 667001718 |a 814379041 |a 814477696 |a 824546069 |a 824560610 |a 1028234338 | ||
| 020 | |a 0585483795 |q (electronic bk.) | ||
| 020 | |a 9780585483795 |q (electronic bk.) | ||
| 020 | |z 019963565X | ||
| 020 | |z 9780199635658 | ||
| 020 | |z 0199635641 | ||
| 020 | |z 9780199635641 | ||
| 029 | 1 | |a AU@ |b 000051574502 | |
| 029 | 1 | |a DEBBG |b BV043137689 | |
| 029 | 1 | |a DEBSZ |b 422404365 | |
| 029 | 1 | |a GBVCP |b 801101700 | |
| 035 | |a (OCoLC)53982369 |z (OCoLC)666955394 |z (OCoLC)667001718 |z (OCoLC)814379041 |z (OCoLC)814477696 |z (OCoLC)824546069 |z (OCoLC)824560610 |z (OCoLC)1028234338 | ||
| 050 | 4 | |a QK495.C9 |b A687 2000eb | |
| 060 | 4 | |a 2007 B-259 | |
| 060 | 4 | |a QK 495.C9 |b A6582 2000 | |
| 070 | |a QK495.C9 |b A687 2000 | ||
| 072 | 7 | |a NAT |x 026000 |2 bisacsh | |
| 072 | 0 | |a F600 | |
| 082 | 0 | 4 | |a 583/.64 |2 22 |
| 084 | |a CW 65(A) |2 blsrissc | ||
| 049 | |a UAMI | ||
| 245 | 0 | 0 | |a Arabidopsis : |b a practical approach / |c edited by Zoe A. Wilson. |
| 260 | |a Oxford ; |a New York : |b Oxford University Press, |c 2000. | ||
| 300 | |a 1 online resource (xix, 275 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 490 | 1 | |a The practical approach series ; |v PAS/223 | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | 0 | |g 1. |t Growth, maintenance, and use of Arabidopsis genetic resources / |r Mary Anderson, Fiona Wilson |g 1 -- |g 1. |t What is Arabidopsis? |g 1 -- |g 2. |t What makes Arabidopsis such an attractive experimental model? |g 3 -- |g 3. |t Arabidopsis genetic resource centres |g 4 -- |t Arabidopsis genetic resources |g 4 -- |t Accessing Arabidopsis resources |g 5 -- |g 4. |t Mutants of Arabidopsis |g 5 -- |t Single gene mutation lines |g 5 -- |t Resources for the identification/investigation of novel genes |g 8 -- |t Mapping tools |g 10 -- |g 5. |t Considerations of available resources for identifying novel genes |g 11 -- |t Forward genetics |g 12 -- |t Reverse genetics |g 14 -- |g 6. |t Growing Arabidopsis |g 15 -- |t How to maintain clean growth conditions |g 15 -- |t Growing Arabidopsis in the glasshouse |g 16 -- |t Chemical control of pests and diseases |g 19 -- |g 7. |t Seed storage |g 21 -- |g 8. |t Growing Arabidopsis with specific growth requirements |g 23 -- |g 9. |t Sterile culture of Arabidopsis |g 24 -- |g 2. |t Preservation and handling of stock centre clones / |r Randy Scholl, Keith Davis, Doreen Ware |g 29 -- |g 2. |t Missions of a plant DNA resource centre |g 29 -- |g 3. |t Preservation of stocks |g 30 -- |t Plasmids with small DNA inserts |g 30 -- |t Cosmids |g 32 -- |t Phage and phage libraries |g 33 -- |t Yeast artificial chromosome (YAC) libraries |g 33 -- |t Pools of YAC library cells for PCR screening |g 37 -- |t Distribution of YAC libraries arrayed on nylon filters |g 38 -- |t Other large-insert libraries |g 38 -- |t Yeast expression analysis--'two-hybrid' libraries and complementation testing |g 42 -- |g 4. |t Verification of stock identity and purity |g 42 -- |g 5. |t Pooled DNA from T-DNA lines for PCR screening |g 44 -- |g 6. |t Organization of stock information |g 48 -- |t Collecting, maintaining, and disseminating stock data |g 48 -- |t Organizing and distributing patron data |g 48 -- |g 7. |t Future |g 48 -- |g 3. |t Genetic mapping using recombinant inbred lines / |r Clare Lister, Mary Anderson, Caroline Dean |g 51 -- |g 2. |t Preparation and digestion of A. thaliana genomic DNA |g 54 -- |t Preparation of genomic DNA |g 56 -- |t Identifying an RFLP |g 60 -- |t Southern blotting and hybridization |g 61 -- |g 3. |t Polymorphic markers |g 62 -- |t RFLP markers |g 62 -- |t PCR-based markers |g 66 -- |t Phenotypic and biochemical markers |g 68 -- |g 4. |t Calculating map positions |g 69 -- |t Mapping programs |g 69 -- |t NASC mapping service |g 70 -- |g 5. |t Integration of a mutation into a molecular map |g 72 -- |g 4. |t Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis / |r Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson |g 77 -- |g 1. |t Generation of mutants and their importance for developmental biology |g 77 -- |g 2. |t Mapping and segregation analysis |g 78 -- |t Mapping of mutations |g 78 -- |t Influence of environment on phenotype |g 82 -- |g 3. |t Microscopy |g 82 -- |t Fresh material characterization |g 82 -- |t Fixed material characterization |g 84 -- |g 4. |t Analysis of plant gene expression |g 89 -- |t RNA isolation |g 89 -- |t Northern analysis |g 91 -- |t In situ hybridizations |g 92 -- |g 5. |t Classical and molecular cytogenetics of Arabidopsis / |r G.H. Jones, J.S. Heslop-Harrison |g 105 -- |g 2. |t Mitotic chromosome analysis by light microscopy |g 108 -- |g 3. |t In situ hybridization to mitotic chromosome preparations |g 112 -- |t Photography of in situ hybridizations |g 117 -- |g 4. |t Meiotic chromosome analysis by light microscopy |g 117 -- |g 5. |t Meiotic chromosome analysis by electron microscopy |g 121 -- |g 6. |t Tissue culture, transformation, and transient gene expression in Arabidopsis / |r Keith Lindsey, Wenbin Wei |g 125 -- |g 2. |t Stable transformation by Agrobacterium tumefaciens |g 128 -- |g 3. |t Transient gene expression in Arabidopsis protoplasts |g 131 -- |t Reporter gene enzyme assays |g 134 -- |g 7. |t Transposon and T-DNA mutagenesis / |r Mark G.M. Aarts, Csaba Koncz, Andy Pereira |g 143 -- |g 2. |t Transposon tagging |g 143 -- |t Endogenous transposable elements |g 143 -- |t Transposon tagging systems in Arabidopsis |g 144 -- |t Which system to use? |g 149 -- |t Genetic and molecular analysis of a putatively transposon tagged mutant |g 151 -- |t Further applications of transposon tagging |g 156 -- |g 3.2 |t T-DNA tagging |g 158 -- |t Use of T-DNA as insertional mutagen |g 158 -- |t Random tagging |g 158 -- |t Available populations of T-DNA transformants |g 159 -- |t Promoter/enhancer trapping |g 160 -- |t Analysis of T-DNA mutants and cloning a tagged gene |g 161 -- |t Further applications of T-DNA tagging |g 166 -- |g 8. |t Map-based cloning in Arabidopsis / |r Joanna Putterill, George Coupland |g 171 -- |g 2. |t Locating the mutation of interest relative to DNA markers |g 172 -- |t Determining an approximate map position |g 172 -- |t Identifying a short genetic interval containing the mutation as a prelude to isolating the gene |g 175 -- |g 3. |t Placing the gene on the physical map |g 177 -- |t Chromosome landing |g 177 -- |t Chromosome walking with YAC clones |g 178 -- |g 4. |t Identification of the gene |g 189 -- |t Location of the gene by molecular complementation |g 189 -- |t Determining the structure of the gene |g 194 -- |g 5. |t Perspectives |g 194 -- |g 9. |t Physical mapping: YACs, BACs, cosmids, and nucleotide sequences / |r Ian Bancroft |g 199 -- |g 2. |t Genome mapping with YAC clones |g 199 -- |g 3. |t Genome mapping with BAC and P1 clones |g 202 -- |t Communal resources |g 202 -- |t Construction of BAC libraries |g 208 -- |t Genome mapping with BACs |g 211 -- |g 4. |t High resolution mapping with cosmids |g 216 -- |t Approaches to mapping with cosmids |g 216 -- |t Construction of cosmid libraries |g 216 -- |g 5. |t Nucleotide sequences--the ultimate mapping tool |g 221 -- |t EST sequencing project |g 221 -- |t Genome sequencing project |g 221 -- |t Sequence-based mapping |g 222 -- |g 10. |t Web-based bioinformatic tools for Arabidopsis researchers / |r Seung Y. Rhee, David J. Flanders |g 225 -- |t What is bioinformatics |g 225 -- |t Sources of Arabidopsis bioinformatic data |g 226 -- |g 2. |t Basic tools for the Internet |g 227 -- |t Web basics |g 227 -- |t Getting onto the Web |g 228 -- |t Using your browser |g 229 -- |t Browser tips and errors |g 232 -- |t Privacy issues |g 235 -- |g 3. |t Scenarios of bioinformatic use in Arabidopsis research |g 235 -- |g 4. |t Gene information resources |g 237 -- |t General gene information |g 237 -- |t Arabidopsis gene information |g 237 -- |t Plant gene information |g 238 -- |t Genetic maps |g 239 -- |t Physical maps |g 240 -- |g 6. |t Sequencing |g 242 -- |t Arabidopsis genome initiative (AGI) |g 243 -- |t Annotation of sequences by AGI |g 243 -- |t Caveats in annotation |g 244 -- |t Sequence contigs from AtDB |g 245 -- |g 7. |t Sequence analysis tools |g 245 -- |t BLAST |g 246 -- |t FASTA |g 250 -- |t EST databases |g 252 -- |t Gene identification programs |g 253 -- |t Gene family analyses |g 255 -- |t Motif analyses |g 255 -- |t Protein structures |g 258 -- |t Comprehensive sequence analysis tools |g 259 -- |g 8. |t Current issues and future directions in bioinformatics |g 260 -- |t Some important bioinformatic issues |g 260 -- |t Bioinformatic tools currently under development for Arabidopsis research. |
| 520 | |a "Arabidopsis has long been acknowledged as the ideal molecular genetic tool for the analysis of development in higher plants. This book covers all the key techniques for using Arabidopsis as an experimental system. This fully up-to-date and comprehensive laboratory manual is an essential source book for all researchers who work on Arabidopsis."--Jacket | ||
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a Arabidopsis |v Laboratory manuals. | |
| 650 | 0 | |a Arabidopsis |x Molecular aspects |v Laboratory manuals. | |
| 650 | 1 | 2 | |a Arabidopsis |x genetics |
| 650 | 2 | 2 | |a Plants, Genetically Modified |x genetics |
| 650 | 6 | |a Arabidopsis |x Manuels de laboratoire. | |
| 650 | 6 | |a Arabidopsis |x Aspect moléculaire |x Manuels de laboratoire. | |
| 650 | 6 | |a Arabidopsis |v Manuels de laboratoire. | |
| 650 | 6 | |a Arabidopsis |x Aspect moléculaire |v Manuels de laboratoire. | |
| 650 | 7 | |a NATURE |x Plants |x General. |2 bisacsh | |
| 650 | 7 | |a Arabidopsis |2 fast | |
| 650 | 7 | |a Arabidopsis. |2 fmesh | |
| 655 | 2 | |a Laboratory Manual | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf | |
| 700 | 1 | |a Wilson, Zoe A. | |
| 776 | 0 | 8 | |i Print version: |t Arabidopsis. |d Oxford ; New York : Oxford University Press, 2000 |z 019963565X |z 0199635641 |w (DLC) 99045851 |w (OCoLC)42295871 |
| 830 | 0 | |a Practical approach series ; |v 223. | |
| 856 | 4 | 0 | |u https://ebsco.uam.elogim.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98516 |z Texto completo |
| 938 | |a YBP Library Services |b YANK |n 20452287 | ||
| 938 | |a ProQuest Ebook Central |b EBLB |n EBL5583491 | ||
| 938 | |a EBSCOhost |b EBSC |n 98516 | ||
| 938 | |a Internet Archive |b INAR |n arabidopsis00zoea | ||
| 938 | |a YBP Library Services |b YANK |n 2336205 | ||
| 994 | |a 92 |b IZTAP | ||