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Signalling by inositides : a practical approach /

"Inositide research has infiltrated numerous branches of biochemistry, physiology, and molecular biology. Consequently, methodological information has been scattered far and wide. This book unites a wide selection of the most fundamental and commonly used techniques. Contributors come from inte...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Shears, Stephen
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Oxford ; New York : IRL Press at Oxford University Press, ©1997.
Colección:Practical approach series.
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • 1. Assaying inositol phospholipid turnover in plant cells-- 2. Measurement of inositol phosphate turnover in intact cells and cell-free systems-- 3. Mass assay of inositol and its use to assay inositol polyphosphates-- 4. Expression, purification and interfacial kinetic analysis of phospholipase C-[gamma]1-- 5. Baculovirus-promoted expression, purification, and functional assay of G-protein regulated PLC-[beta] isoenzymes-- 6. Purification and assay of PLC-Ơ-- 7. The Purification and assay of inositide binding proteins.
  • 8. Mass assay of inositol 1,4,5-triphosphate and phospatidylinositol 4,5-bisphospate-- 9. Desalting inositol polyphospates by dialysis-- 10. The Ins(1,4,5)P₃ receptor-- 11. Purification and assay of inositol hexakisphosphate kinase and diphosphoinositol pentakisphosphate kinase-- 12. Phosphoinositide 3-kinase-- A1. List of suppliers-- A2. List of suppliers of inositol derivatives.