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Post-translational processing : a practical approach /
Post - Translational Modification: A Practical Approach and its companion volume Protein Expression: A Practical Approach form the final part of the PAS mini-series on protein synthesis and processing. This volume begins with a chapter on protein sequencing followed by a chapter on protein folding a...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford ; New York :
Oxford University Press,
1999.
|
| Colección: | Practical approach series ;
203. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Post-translational processing : |b a practical approach / |c edited by S.J. Higgins and B.D. Hames. |
| 260 | |a Oxford ; |a New York : |b Oxford University Press, |c 1999. | ||
| 300 | |a 1 online resource (xx, 317 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Practical approach series ; |v 203 | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 520 | |a Post - Translational Modification: A Practical Approach and its companion volume Protein Expression: A Practical Approach form the final part of the PAS mini-series on protein synthesis and processing. This volume begins with a chapter on protein sequencing followed by a chapter on protein folding and import into organelles. The next three chapters cover the three major forms of covalent modification: phosphorylation, glycosylation, and lipid modification. Proteolytic processing the is the next topic and the final two chapters are concerned with protein turnover in mammalian cells and yeast. This book is a comprehensive volume of the best current methodology and is designed to be used at the bench or away from the bench to gain insight into future experimental approached. | ||
| 505 | 0 | |a Intro; Contents; List of Contributors; Abbreviations; 1. Sequence analysis of expressed proteins; 1. Introduction; 2. N-terminal sequence analysis; Automated sequencing; 3. Sample preparation; Sample preparation by SDS-PAGE; Sample preparation by HPLC; Other procedures for sample preparation; 4. N-terminal blocking; Protein fragmentation; 5. C-terminal sequencing; Chemical analysis of the C-terminus; Enzymic analysis of the C-terminus; 6. Mass spectrometric analysis of proteins; A brief guide to mass spectrometers; Molecular mass determination; Sequencing by mass spectrometry; References | |
| 505 | 8 | |a 2. Protein folding and import into organelles1. Introduction; 2. Preparation of preprotein substrates for in vitro import into organelles; Preparation of mRNA for in vitro translation; Synthesis of preproteins in the reticulocyte lysate; Synthesis of preproteins in wheat germ lysate; Synthesis of preproteins in yeast cytosol; Synthesis of preproteins in Escherichia coli; Dihydrofolate reductase as a model protein for import studies; 3. Import of proteins into mitochondria; Isolation of mitochondria for import studies; Import of preproteins into isolated mitochondria | |
| 505 | 8 | |a Generation of translocation intermediatesLocalization of imported proteins; 4. Import of proteins into microsomes (endoplasmic reticulum); Isolation of microsomes; Import of preproteins into microsomes; 5. Import of proteins into other organelles; 6. Analysis of protein import into organelles; Monitoring the association of proteins with membranes; Analysis of protein complexes involved in organelle import; Analysis of protein folding after import into organelles; Acknowledgements; References; 3. Analysis of protein phosphorylation; 1. Introduction | |
| 505 | 8 | |a 2. Investigating protein phosphorylation systems3. Phosphorylation of proteins in intact preparations; General considerations; Intact animals; Tissue slices; Isolated cells; 4. Phosphorylation of proteins in cell-free preparations; General considerations; Labelling and stimulation of cell-free preparations; 5. Analysis of phosphorylated proteins; General considerations; Quantification of phosphoproteins; Phosphoprotein separation; Protein isolation by immunomethods; Analysis of multisite phosphorylation; Phosphoamino acid analysis; Analysis of the state of phosphorylation | |
| 505 | 8 | |a 6. Analysis of protein kinasesGeneral considerations; Analysis of protein kinase activity in vitro; Analysis of specific protein kinases in vitro; Analysis of protein kinase activity in intact cells; Purification of protein kinases; 7. Phosphoprotein phosphatases; General considerations; P-Ser/P-Thr phosphoprotein phosphatases; Tyrosine-specific phosphoprotein phosphatases; References; 4. Protein glycosylation; 1. Introduction; Protein glycosylation; Oligosaccharide structures; Glycosylation pathways; Characterization of protein glycosylation; 2. Is my protein glycosylated? | |
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a Post-translational modification. | |
| 650 | 2 | |a Protein Biosynthesis | |
| 650 | 2 | |a Peptide Biosynthesis | |
| 650 | 6 | |a Protéines |x Modification post-traductionnelle. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Post-translational modification. |2 fast |0 (OCoLC)fst01072761 | |
| 650 | 7 | |a Protéines |x Modifications posttraductionnelles. |2 ram | |
| 700 | 1 | |a Higgins, S. J. |q (Steve J.) | |
| 700 | 1 | |a Hames, B. D. | |
| 776 | 0 | 8 | |i Print version: |t Post-translational processing. |d Oxford ; New York : Oxford University Press, 1999 |z 0199637946 |z 0199637954 |w (DLC) 98052159 |w (OCoLC)40489139 |
| 830 | 0 | |a Practical approach series ; |v 203. | |
| 856 | 4 | 0 | |u https://ebsco.uam.elogim.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98471 |z Texto completo |
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