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Mutation detection : a practical approach /
Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mu...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford ; New York :
IRL Press at Oxford University Press,
©1998.
|
| Colección: | Practical approach series ;
188. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Mutation detection : |b a practical approach / |c edited by R.G.H. Cotton, E. Edkins, and S. Forrest. |
| 260 | |a Oxford ; |a New York : |b IRL Press at Oxford University Press, |c ©1998. | ||
| 300 | |a 1 online resource (xx, 242 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 1 | |a The practical approach series ; |v 188 | |
| 504 | |a Includes bibliographical references and index. | ||
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 520 | |a Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers tried and tested protocols for a range of detection methods, from the labs of researchers in the field. | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Cover -- Contents -- List of Contributors -- Abbreviations -- Introduction -- References -- 1. Single-strand conformation polymorphism analysis -- 1. Introduction -- 2. PCR-SSCP using polyacrylamide slab gel -- PCR Optimization and primer design -- Pre-amplification and isolation by agarose gel electrophoresis -- PCR using [[Sup(32)]P]deoxynucleotide triphosphate -- Removal of 3' appendage -- SSCP gel electrophoresis -- Interpretation of autoradiogram -- Re-amplification and direct sequencing -- Gel matrices other than polyacrylamide. | |
| 505 | 8 | |a Restriction endonuclease fingerprinting and dideoxy fingerprinting -- 3. Fluorescent SSCP in an automated DNA sequencer -- Primer design in post-PCR fluorescent labelling -- Fluorescent labelling by 3' exchange reaction -- SSCP in capillary electrophoresis (CE-SSCP) -- Data processing -- Acknowledgements -- References -- 2. Single-stranded conformation polymorphism and heteroduplex analysis -- 1. Introduction -- 2. Optimization of the PCR reaction -- 3. SSCP sample prepration -- 4. Optimization of SSCP/HA detection -- 5. Multiplexing -- 6. Interpretation of results -- 7. Applications. | |
| 505 | 8 | |a 8. Other methods -- References -- 3. Comprehensive mutation detection with denaturing gradient gel electrophoresis -- 1. Introduction -- The scope of DGGE, its distinctive capabilities, and the nature of results -- 2. Background -- 3. Basic principle, the physical properties of DNA -- 4. Overview of the procedures in searching for mutants -- Defining segments for scrutiny -- Sample preparation -- Gradient and velocity separations -- Features of the gel patterns -- Discrimination of zygozygosity -- Comments -- 5. Use of the psoralen cross-link as a clamp -- The psoralen protocol. | |
| 505 | 8 | |a 6. Computational tools -- What is a meltmap? -- Meltmap protocol -- Predicting electrophoretic separations -- Computer operations for MUTRAV -- 7. Other members of the DGGE family -- Gel separations in a uniform, partially denaturing environment -- Capillary electrophoresis -- The thermal gradient -- The temperature ramp -- 2D length and gradient separations -- 8. End notes -- Acknowledgments -- References -- 4. Cleavage using RNase to detect mutations -- 1. Introduction -- 2. RNase protection assay for mutation detection -- Evaluation of the sensitivity -- Source material. | |
| 505 | 8 | |a PCR for RNase protection assay -- RNA probe preparation -- RNase protection -- Detection of digested probe -- Mutation detection by sequencing of the PCR products -- Other modified methodologies for mutation detection -- Acknowledgements -- References -- 5. Cleavage of mismatched bases using chemical reagents -- 1. Introduction -- 2. Basic procedures -- Comments on the basic procedures -- 3. Ultra fast chemical mismatch detection -- Labelling -- Solid phase -- Comments -- References -- 6. Mutation detection using T4 endonuclease VII -- 1. Introduction -- 2. The biology of Endo VII. | |
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a Mutation (Biology) |v Laboratory manuals. | |
| 650 | 0 | |a Molecular genetics |v Laboratory manuals. | |
| 650 | 0 | |a Chromosome abnormalities |v Laboratory manuals. | |
| 650 | 0 | |a Mutation (Biology) | |
| 650 | 0 | |a Chromosome abnormalities. | |
| 650 | 2 | |a Mutation | |
| 650 | 2 | |a Molecular Biology | |
| 650 | 2 | |a Chromosome Aberrations | |
| 650 | 6 | |a Mutation (Biologie) |v Manuels de laboratoire. | |
| 650 | 6 | |a Génétique moléculaire |v Manuels de laboratoire. | |
| 650 | 6 | |a Aberrations chromosomiques |v Manuels de laboratoire. | |
| 650 | 6 | |a Mutation (Biologie) | |
| 650 | 6 | |a Aberrations chromosomiques. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Genetics & Genomics. |2 bisacsh | |
| 650 | 7 | |a Chromosome abnormalities. |2 fast |0 (OCoLC)fst00860059 | |
| 650 | 7 | |a Molecular genetics. |2 fast |0 (OCoLC)fst01024797 | |
| 650 | 7 | |a Mutation (Biology) |2 fast |0 (OCoLC)fst01031152 | |
| 650 | 7 | |a Genmutation |2 gnd | |
| 650 | 7 | |a Nachweis |2 gnd | |
| 655 | 2 | |a Laboratory Manual | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf | |
| 700 | 1 | |a Cotton, Richard G. H. | |
| 700 | 1 | |a Edkins, E. |q (Edward) | |
| 700 | 1 | |a Forrest, S. |q (Sue) | |
| 776 | 0 | 8 | |i Print version: |t Mutation detection. |d Oxford ; New York : IRL Press at Oxford University Press, ©1998 |z 0199636575 |z 0199636567 |w (DLC) 97051537 |w (OCoLC)38249893 |
| 830 | 0 | |a Practical approach series ; |v 188. | |
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