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Virus culture : a practical approach /
Viruses have properties that are distinct from other living organisms and so require different methods to culture them. This volume provides a broad treatment of the principles and practice of virus culture and will be of interest to all those involved in virus culture including academic, industrial...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford ; New York :
Oxford University Press,
©1999.
|
| Colección: | Practical approach series ;
208. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Virus culture : |b a practical approach / |c edited by Alan J. Cann. |
| 260 | |a Oxford ; |a New York : |b Oxford University Press, |c ©1999. | ||
| 300 | |a 1 online resource (xviii, 272 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Practical approach series ; |v 208 | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Intro; Contents; List of Contributors; Abbreviations; 1. Cell culture; 1. Introduction; Sources of cell cultures; Suppliers of cell cultures; Types of cell culture; 2. Culture media; Basal media; Serum; Serum-free media; Antibiotics; 3. Good practice in cell culture; 4. Principles of cell banking; 5. Subculture of cells; 6. Quantification of cell cultures; 7. Cryopreservation and storage of cell lines; Storage of cryopreserved cell stocks; Resuscitation of cryopreserved cell stocks; 8. Quality control testing of cell lines; Preparation of cell cultures prior to testing; Mycoplasma | |
| 505 | 8 | |a Bacteria and fungiViruses; Isoenzyme analysis; Karyotyping; DNA fingerprinting; 9. Transportation; Shipment of growing cultures; Shipment of frozen materials on dry ice (solid carbon dioxide); References; 2. Virus isolation; 1. Why culture viruses?; 2. Viruses not amenable to cultivation; 3. Treatment of clinical specimens for virus isolation; 4. Identification of virus replication; 5. Passaging of viruses; 6. Confirmation of virus isolation; 7. Adaptations of cell culture; Haemadsorption test; Detection of early antigen fluorescent foci (DEAFF test); 8. Conclusions; References | |
| 505 | 8 | |a 3. Concentration and purification of viruses1. The need for virus concentration and purification; Practical considerations; 2. Virus concentration: general principles; 3. Purification of virus: identification of virus-containing fractions; 4. Purification by ultracentrifugation; Types of ultracentrifugation gradient; Forming gradients; 5. Assessment of purity; References; 4. Assays for virus infection; 1. Introduction; 2. Identification of viruses isolated in cell culture; Cytopathic effects; Virus infectivity assays; Immunofluorescence; Electron microscopy | |
| 505 | 8 | |a Other methods of identifying viruses growing in cell cultureDetection of virus pre-cytopathic effects; Detection of viruses not producing a cytopathic effect; 3. Virus antigen detection; Immunofluorescence; Particle agglutination; Antigen-capture ELISA; 4. Virus genome detection; Nucleic acid extraction; Genome detection assays; Quantitative nucleic acid amplification techniques; 5. Detection of specific anti-viral antibodies; Virus-specific IgM detection; Detection of virus-specific IgG; References; 5. Electron microscopy of viruses; 1. Introduction | |
| 505 | 8 | |a 2. Virus structure and strategies for virus detection3. Specimen preparation; Centrifugation; Ultracentrifugation; Ultrafiltration; Precipitation; Bioaffinity techniques; 4. EM grids and support films; 5. Negative staining; Negative stains: properties; Negative staining procedures; Quantitative aspects: measuring and counting in TEM; 6. Embedding and thin sectioning; Fixation and staining; Embedding; Thin sectioning; Post-staining; 7. Immuno-electron microscopy (IEM); Antibodies: properties and usage; Immuno-negative staining; Immuno-labelling of thin sections | |
| 520 | |a Viruses have properties that are distinct from other living organisms and so require different methods to culture them. This volume provides a broad treatment of the principles and practice of virus culture and will be of interest to all those involved in virus culture including academic, industrial, and clinical research groups. | ||
| 546 | |a English. | ||
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a Virology |x Cultures and culture media. | |
| 650 | 0 | |a Monoclonal antibodies. | |
| 650 | 0 | |a Viral vaccines. | |
| 650 | 1 | 2 | |a Virology |x methods |
| 650 | 2 | |a Virus Cultivation | |
| 650 | 2 | 2 | |a Antibodies, Monoclonal |
| 650 | 2 | 2 | |a Antiviral Agents |x pharmacology |
| 650 | 2 | 2 | |a Microscopy, Electron |x methods |
| 650 | 2 | 2 | |a Plant Viruses |x isolation & purification |
| 650 | 2 | 2 | |a Viral Vaccines |
| 650 | 2 | 2 | |a Virus Cultivation |x methods |
| 650 | 6 | |a Virologie |x Cultures et milieux de culture. | |
| 650 | 6 | |a Anticorps monoclonaux. | |
| 650 | 6 | |a Vaccins antiviraux. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biology. |2 bisacsh | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Microbiology. |2 bisacsh | |
| 650 | 7 | |a Viral vaccines |2 fast | |
| 650 | 7 | |a Monoclonal antibodies |2 fast | |
| 650 | 7 | |a Virology |x Cultures and culture media |2 fast | |
| 650 | 7 | |a Zellkultur |2 gnd | |
| 650 | 7 | |a Methode |2 gnd | |
| 650 | 7 | |a Viren |2 gnd | |
| 700 | 1 | |a Cann, Alan. | |
| 776 | 0 | 8 | |i Print version: |t Virus culture. |d Oxford ; New York : Oxford University Press, ©1999 |z 0199637156 |z 0199637148 |w (DLC) 99019983 |w (OCoLC)40805517 |
| 830 | 0 | |a Practical approach series ; |v 208. | |
| 856 | 4 | 0 | |u https://ebsco.uam.elogim.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98442 |z Texto completo |
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