Cargando…
Chromosome structural analysis : a practical approach /
The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (for example, histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information for m...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford ; New York :
Oxford University Press,
1999.
|
| Colección: | Practical approach series ;
200. |
| Temas: | |
| Acceso en línea: | Texto completo |
| Sumario: | The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (for example, histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information for many aspects of cellular genetics such as DNA replication, The book is structured in a methodical fashion - the introductory chapters are centred around analysis of chromatin with chapters on the mapping of protein: DNA interactions "in vivo" using ligation-mediated PCR and the mapping of chromatin-associated proteins by formaldehyde cross-linking. The next chapters concentrate on the study of whole chromosome structure, including: fission yeast chromosome analysis using FISH and CHIP, isolation of vertebrate metaphase chromosomes and their analysis by FISH, the study of vertebrate chromosome progression through mitosis, and the analysis of mammalian interphase chromosomes by immunofluorescence and FISH. There then follow chapters on FISH in whole-mount tissues and the analysis of the sub-structure of mammalian nuclei "in vitro." |
|---|---|
| Descripción Física: | 1 online resource (xxiii, 209 pages) : illustrations (some color) |
| Bibliografía: | Includes bibliographical references and index. |
| ISBN: | 0585484090 9780585484099 1280375590 9781280375590 9786610375592 6610375593 9786610756391 6610756392 9780191565809 0191565806 |