Bioanalytical Chemistry

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Detalles Bibliográficos
Autor principal: Mikkelsen, Susan R.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Newark : John Wiley & Sons, Incorporated, 2016.
Colección:New York Academy of Sciences Ser.
Temas:
Electronic books.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • Title Page
  • Copyright
  • Preface to Second Edition
  • Preface to First Edition
  • Acknowledgments
  • 1: Quantitative Instrumental Measurements
  • 1.1 Introduction
  • 1.2 Optical Measurements
  • 1.3 Electrochemical Measurements
  • 1.4 Radiochemical Measurements
  • 1.5 Surface Plasmon Resonance
  • 1.6 Calorimetry
  • 1.7 Automation: Microplates, Multiwell Liquid Dispensers and Microplate Readers
  • 1.8 Calibration of Instrumental Measurements
  • 1.9 Quantitative and Semi-Quantitative Measurements
  • Suggested Reading
  • Problems
  • 2: Spectroscopic Methods for the Quantitation of Classes of Biomolecules
  • 2.1 Introduction
  • 2.2 Total Protein
  • 2.3 Total DNA
  • 2.4 Total RNA
  • 2.5 Total Carbohydrate
  • 2.6 Free Fatty Acids
  • References
  • Problems
  • 3: Enzymes
  • 3.1 Introduction
  • 3.2 Enzyme Nomenclature
  • 3.3 Enzyme Commission Numbers
  • 3.4 Enzymes in Bioanalytical Chemistry
  • 3.5 Enzyme Kinetics
  • 3.6 Enzyme Activators
  • 3.7 Enzyme Inhibitors
  • 3.8 Enzyme Units and Concentrations
  • Suggested Reading
  • References
  • Problems
  • 4: Quantitation of Enzymes and Their Substrates
  • 4.1 Introduction
  • 4.2 Substrate Depletion or Product Accumulation
  • 4.3 Direct and Coupled Measurements
  • 4.4 Classification of Methods
  • 4.5 Instrumental Methods
  • 4.6 High-Throughput Assays for Enzymes and Inhibitors
  • 4.7. Assays for Enzymatic Reporter Gene Products27-30
  • 4.8 Practical Considerations for Enzymatic Assays
  • Suggested Reading
  • References
  • Problems
  • 5: Immobilized Enzymes
  • 5.1 Introduction
  • 5.2 Immobilization Methods
  • 5.3 Properties of Immobilized Enzymes
  • 5.4 Immobilized Enzyme Reactors
  • 5.5 Theoretical Treatment of Packed-Bed Enzyme Reactors34-37
  • Suggested Reading
  • 8.1 Introduction
  • 8.2 Biosensor Diversity and Classification
  • 8.3 Recognition Agents
  • 8.4 Response of Enzyme-Based Biosensors5
  • 8.5 Examples of Biosensor Configurations
  • 8.6 Evaluation of Biosensor Perfomance
  • 8.7 In VIVO Applications of Biosensors
  • Suggested Reading
  • References
  • Problems
  • 9: Directed Evolution for the Design of Macromolecular Reagents
  • 9.1 Introduction
  • 9.2 Rational Design and Directed Evolution
  • 9.3 Generation of Genetic Diversity
  • 9.4 Linking Genotype and Phenotype
  • 9.5 Identification and Selection of Successful Variants

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