Culture of Animal Cells A Manual of Basic Technique and Specialized Applications.

Detalles Bibliográficos
Autor principal: Freshney, R. Ian
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Newark : John Wiley & Sons, Incorporated, 2016.
Colección:New York Academy of Sciences Ser.
Temas:
Electronic books.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • CULTURE OF ANIMAL CELLS
  • Contents
  • List of Figures
  • List of Color Plates
  • List of Tables
  • List of Protocols
  • List of Minireviews
  • Preface and Acknlowledgments
  • Abbreviations
  • About the Companion Website
  • 1. Introduction
  • 1.1 Historical Background
  • 1.2 Advantages of Tissue Culture
  • 1.2.1 Control of the Environment
  • 1.2.2 Characterization and Homogeneity of Sample
  • 1.2.3 Economy, Scale, and Mechanization
  • 1.2.4 In vitro Modeling of In vivo Conditions
  • 1.3 Limitations
  • 1.3.1 Expertise
  • 1.3.2 Quantity
  • 1.3.3 Dedifferentiation and Selection
  • 1.3.4 Origin of Cells
  • 1.3.5 Instability
  • 1.4 Major Differences In Vitro
  • 1.5 Types of Tissue Culture
  • References
  • 2. Biology of Cultured Cells
  • 2.1 The Culture Environment
  • 2.2 Cell Adhesion
  • 2.2.1 Cell Adhesion Molecules
  • 2.2.2 Intercellular Junctions
  • 2.2.3 Cytoskeleton
  • 2.2.4 Extracellular Matrix
  • 2.2.5 Cell Motility
  • 2.3 Cell Proliferation
  • 2.3.1 Cell Cycle
  • 2.3.2 Control of Cell Proliferation
  • 2.4 Differentiation
  • 2.4.1 Induction and Maintenance of Differentiation
  • 2.4.2 Plasticity of Differentiation and Dedifferentiation
  • 2.5 Cell Signaling
  • 2.6 Energy Metabolism
  • 2.7 Origin of Cultured Cells
  • 2.7.1 Initiation of the Culture
  • 2.7.2 Evolution of Cell Lines
  • 2.7.3 Senescence
  • 2.7.4 Transformation and the Development of Continuous Cell Lines
  • 2.8 Definitions
  • References
  • Supplier
  • 3. Laboratory Design and Layout
  • 3.1 Planning, Furnishing, and Services
  • 3.1.1 Requirements
  • 3.1.2 Services
  • 3.1.3 Ventilation
  • 3.2 Design and Layout
  • 3.2.1 Sterile Handling Area
  • 3.2.2 Laminar Flow Hoods
  • 3.2.3 Service Bench
  • 3.2.4 Quarantine and Containment
  • 3.2.5 Incubation
  • 3.2.6 Preparation Area
  • 3.2.7 Storage
  • 3.3 Disaster Management
  • References
  • 4. Equipment and Materials
  • 4.1 Requirements of a Tissue Culture Laboratory
  • 4.2 Sterile Working Area
  • 4.2.1 Laminar-Flow BSC
  • 4.2.2 Service Carts
  • 4.2.3 Sterile Liquid Handling-Pipetting and Dispensing
  • 4.2.4 Inverted Microscope
  • 4.2.5 Camera and Monitor
  • 4.2.6 Dissecting Microscope
  • 4.2.7 Centrifuge
  • 4.2.8 Cell Counting
  • 4.3 Incubation and Culture
  • 4.3.1 Incubator
  • 4.3.2 Humid CO2 Incubator
  • 4.3.3 Temperature Recorders
  • 4.3.4 Roller Racks
  • 4.3.5 Magnetic Stirrer
  • 4.3.6 Culture Vessels
  • 4.4 Preparation and Sterilization
  • 4.4.1 Washup
  • 4.4.2 Preparation of Media and Reagents
  • 4.4.3 Sterilization
  • 4.5 Storage
  • 4.5.1 Consumables
  • 4.5.2 Refrigerators and Freezers
  • 4.5.3 Cryostorage Containers
  • 4.5.4 Controlled-Rate Freezer
  • 4.6 Supplementary Laboratory Equipment
  • 4.6.1 Computers and Networks
  • 4.6.2 Upright Microscope
  • 4.6.3 Low-Temperature Freezer
  • 4.6.4 Confocal Microscope
  • 4.6.5 PCR Thermal Cycler
  • 4.7 Specialized Equipment
  • 4.7.1 Microinjection Facilities
  • 4.7.2 Colony Counter
  • 4.7.3 Centrifugal Elutriator
  • 4.7.4 Flow Cytometer
  • References
  • Suppliers
  • 5. Aseptic Technique

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