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EBOOKCENTRAL_on1347027129 |
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OCoLC |
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20240329122006.0 |
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m o d |
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cr cnu|||||||| |
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230209s2016 xx o ||| 0 eng d |
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|a EBLCP
|b eng
|c EBLCP
|d OCLCQ
|d OCLCO
|d OCLCQ
|d EBLCP
|d OCLCQ
|d OCLCL
|d OCLCQ
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| 020 |
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|a 9781118873373
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| 020 |
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|a 1118873378
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| 035 |
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|a (OCoLC)1347027129
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| 082 |
0 |
4 |
|a 571.5/38
|q OCoLC
|2 23/eng/20230216
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| 049 |
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|a UAMI
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| 100 |
1 |
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|a Freshney, R. Ian.
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| 245 |
1 |
0 |
|a Culture of Animal Cells
|h [electronic resource] :
|b A Manual of Basic Technique and Specialized Applications.
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| 260 |
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|a Newark :
|b John Wiley & Sons, Incorporated,
|c 2016.
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| 300 |
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|a 1 online resource (758 p.).
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| 490 |
1 |
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|a New York Academy of Sciences Ser.
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| 500 |
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|a Description based upon print version of record.
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| 505 |
0 |
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|a Intro -- CULTURE OF ANIMAL CELLS -- Contents -- List of Figures -- List of Color Plates -- List of Tables -- List of Protocols -- List of Minireviews -- Preface and Acknlowledgments -- Abbreviations -- About the Companion Website -- 1. Introduction -- 1.1 Historical Background -- 1.2 Advantages of Tissue Culture -- 1.2.1 Control of the Environment -- 1.2.2 Characterization and Homogeneity of Sample -- 1.2.3 Economy, Scale, and Mechanization -- 1.2.4 In vitro Modeling of In vivo Conditions -- 1.3 Limitations -- 1.3.1 Expertise -- 1.3.2 Quantity -- 1.3.3 Dedifferentiation and Selection
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| 505 |
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|a 1.3.4 Origin of Cells -- 1.3.5 Instability -- 1.4 Major Differences In Vitro -- 1.5 Types of Tissue Culture -- References -- 2. Biology of Cultured Cells -- 2.1 The Culture Environment -- 2.2 Cell Adhesion -- 2.2.1 Cell Adhesion Molecules -- 2.2.2 Intercellular Junctions -- 2.2.3 Cytoskeleton -- 2.2.4 Extracellular Matrix -- 2.2.5 Cell Motility -- 2.3 Cell Proliferation -- 2.3.1 Cell Cycle -- 2.3.2 Control of Cell Proliferation -- 2.4 Differentiation -- 2.4.1 Induction and Maintenance of Differentiation -- 2.4.2 Plasticity of Differentiation and Dedifferentiation -- 2.5 Cell Signaling
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| 505 |
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|a 2.6 Energy Metabolism -- 2.7 Origin of Cultured Cells -- 2.7.1 Initiation of the Culture -- 2.7.2 Evolution of Cell Lines -- 2.7.3 Senescence -- 2.7.4 Transformation and the Development of Continuous Cell Lines -- 2.8 Definitions -- References -- Supplier -- 3. Laboratory Design and Layout -- 3.1 Planning, Furnishing, and Services -- 3.1.1 Requirements -- 3.1.2 Services -- 3.1.3 Ventilation -- 3.2 Design and Layout -- 3.2.1 Sterile Handling Area -- 3.2.2 Laminar Flow Hoods -- 3.2.3 Service Bench -- 3.2.4 Quarantine and Containment -- 3.2.5 Incubation -- 3.2.6 Preparation Area -- 3.2.7 Storage
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| 505 |
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|a 3.3 Disaster Management -- References -- 4. Equipment and Materials -- 4.1 Requirements of a Tissue Culture Laboratory -- 4.2 Sterile Working Area -- 4.2.1 Laminar-Flow BSC -- 4.2.2 Service Carts -- 4.2.3 Sterile Liquid Handling-Pipetting and Dispensing -- 4.2.4 Inverted Microscope -- 4.2.5 Camera and Monitor -- 4.2.6 Dissecting Microscope -- 4.2.7 Centrifuge -- 4.2.8 Cell Counting -- 4.3 Incubation and Culture -- 4.3.1 Incubator -- 4.3.2 Humid CO2 Incubator -- 4.3.3 Temperature Recorders -- 4.3.4 Roller Racks -- 4.3.5 Magnetic Stirrer -- 4.3.6 Culture Vessels -- 4.4 Preparation and Sterilization
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| 505 |
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|a 4.4.1 Washup -- 4.4.2 Preparation of Media and Reagents -- 4.4.3 Sterilization -- 4.5 Storage -- 4.5.1 Consumables -- 4.5.2 Refrigerators and Freezers -- 4.5.3 Cryostorage Containers -- 4.5.4 Controlled-Rate Freezer -- 4.6 Supplementary Laboratory Equipment -- 4.6.1 Computers and Networks -- 4.6.2 Upright Microscope -- 4.6.3 Low-Temperature Freezer -- 4.6.4 Confocal Microscope -- 4.6.5 PCR Thermal Cycler -- 4.7 Specialized Equipment -- 4.7.1 Microinjection Facilities -- 4.7.2 Colony Counter -- 4.7.3 Centrifugal Elutriator -- 4.7.4 Flow Cytometer -- References -- Suppliers -- 5. Aseptic Technique
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| 500 |
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|a 5.1 Objectives of Aseptic Technique
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| 590 |
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|a ProQuest Ebook Central
|b Ebook Central Academic Complete
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| 655 |
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0 |
|a Electronic books.
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| 758 |
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|i has work:
|a Culture of animal cells (Work)
|1 https://id.oclc.org/worldcat/entity/E39PD3vrDPdwCTGfRCtdfm9fVP
|4 https://id.oclc.org/worldcat/ontology/hasWork
|
| 776 |
0 |
8 |
|i Print version:
|a Freshney, R. Ian
|t Culture of Animal Cells
|d Newark : John Wiley & Sons, Incorporated,c2016
|z 9781118873656
|
| 830 |
|
0 |
|a New York Academy of Sciences Ser.
|
| 856 |
4 |
0 |
|u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=7104162
|z Texto completo
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| 938 |
|
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|a ProQuest Ebook Central
|b EBLB
|n EBL7104162
|
| 994 |
|
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|a 92
|b IZTAP
|
