Microbial production of cis,cis-muconic acid from hydrothermally converted lignocellulose

Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Starck, Sören
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Göttingen : Cuvillier Verlag, 2022.
Colección:Hochschulschriften - Institut für Systembiotechnologie, Universität des Saarlandes.
Temas:
Hydrolysis.
Hydrolyse.
hydrolysis.
Hydrolysis
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • Summary
  • Zusammenfassung
  • 1 Introduction
  • 1.1 General introduction
  • 1.2 Objectives
  • 2 Theoretical Background
  • 2.1 Biomass as a resource
  • 2.2 Depolymerization of biomass
  • 2.3 Bioconversion of biomass-derived carbon
  • 2.4 Cis,cis-muconic acid
  • an important platform chemical
  • 3 Material and Methods
  • 3.1 Lignin and hemicellulose
  • 3.2 Hydrothermal lignin conversion
  • 3.3 Hydrothermal lignin conversion at miniaturized scale
  • 3.4 Hydrothermal hemicellulose conversion
  • 3.5 Fractionation of depolymerized hemicellulose by distillation
  • 3.6 Distillation of lignin hydrolysate
  • 3.7 Concentration of lignin and hemicellulose hydrolysates
  • 3.8 Decolorization of hemicellulose hydrolysates and removal oftoxic ingredients
  • 3.9 Microorganisms and plasmids
  • 3.10 Media
  • 3.11 Cultivation of Pseudomonas putida
  • 3.12 Cultivation of Corynebacterium glutamicum strains
  • 3.13 Cultivation of Amycolatopsis sp.
  • 3.14 Fed-batch production of cis,cis-muconic acid
  • 3.15 Quantification of glucose and xylose
  • 3.16 Quantification of mono- and disaccharides
  • 3.17 Quantification of aromatics and muconic acid derivatives
  • 3.18 Fingerprinting of aromatics in lignin hydrolysates
  • 3.19 Quantification of organic acids and alcohols
  • 3.20 Quantification of cell concentration
  • 4 Results and Discussion
  • 4.1 Microbial production of cis,cis-muconic acid from aromatics
  • 4.2 Cascaded production of cis,cis-muconic acid from Kraft lignin
  • 4.3 Impact of the lignin-type on depolymerization and bioconversionefficiency
  • 4.4 Gram-scale production of cis,cis-muconic acid from repeatedfeeding of lignin hydrolysate
  • 4.5 The use of hemicellulose-derived sugars as co-substrate enablesan entirely lignocellulose-based process
  • 5 Conclusion and Outlook
  • 6 Appendix
  • 6.1 Abbreviations
  • 6.2 Cultivation of P. putida MA-9 on organosolv lignin hydrolysate
  • 6.3 Aromatic monomers obtained from multiple runs of hydrothermal
  • 6.4 Cultivation of C. glutamicum MA-3 on hemicellulose hydrolysatein a bioreactor with addition of lignin hydrolysate
  • 6.5 Overview of results from hydrothermal conversion of lignin andsubsequent bioconversion to muconic acids
  • 7 References

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