Advanced Diffusion Studies of Active Enzymes and Nanosystems

Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Günther, Jan-Philipp
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Göttingen : Cuvillier Verlag, 2021.
Temas:
Enzymes.
Aldolase.
Nanotechnology.
Alkaline phosphatase.
Enzymes
Alkaline Phosphatase
Nanotechnology
Phosphatase alcaline.
Nanotechnologie.
enzyme.
Aldolase
Alkaline phosphatase
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Intro
  • Table of Contents
  • Abbreviations
  • Abstract
  • Kurzzusammenfassung
  • 1 Introduction
  • 1.1 Publications
  • 2 Motion at the Nanoscale
  • 2.1 Motion at Low Reynolds Numbers
  • 2.2 Brownian Motion
  • 3 Active Brownian motion
  • 3.1 Self-Phoresis of Micro- and Nanoparticles
  • 3.2 Active Diffusion Hypothesis for Enzymes
  • 3.3 Molecular Catalysts & Tracer Diffusion
  • 4 Diffusion Measurement Techniques
  • 4.1 Fluorescence Correlation Spectroscopy
  • 4.1.1 Single Molecule Fluorescence
  • 4.1.2 Fluorescence Detection from a Confocal volume
  • 4.1.3 Autocorrelation Function and Fitting
  • 4.2 Nuclear Magnetic Resonance Spectroscopy
  • 4.2.1 Nuclear Magnetization
  • 4.2.2 Pulsed Field Gradients
  • 4.2.3 Advanced Pulse Sequences
  • 4.3 Other Techniques
  • 4.3.1 Other Correlation Methods
  • 4.3.2 Tracking Microscopy
  • 4.3.3 Trapping Methods
  • 4.3.4 Mutual Diffusion Measurement Techniques
  • 4.3.5 Computational Methods
  • 5 Enzymes Properties
  • 5.1 Alkaline Phosphatase
  • 5.2 Fructose-Bisphosphate Aldolase
  • 5.3 Urease
  • 5.4 FoF1-ATP synthase
  • 6 Applications of Enzymes
  • 6.1 Biocatalysis
  • 6.2 Immobilization
  • 6.3 Collective Effects
  • 7 Misinterpretation of Enzyme FCS
  • 7.1 Conspectus
  • 7.2 Introduction
  • 7.3 Experiment and Theory of the Enhanced Diffusion of ActiveEnzymes
  • 7.4 Fluorescence Correlation Spectroscopy
  • 7.5 Enhanced Diffusion of F1-ATPase
  • 7.6 Effects and Artefacts in FCS Measurements of ProteinDiffusion
  • 7.7 Suggestions for Future FCS Experiments of Enzyme Diffusion
  • 7.8 Fluorescence Quenching of Alkaline Phosphatase
  • 7.9 Conclusions
  • 7.10 Acknowledgment
  • 8 Label-Free Enzyme Diffusion Measurements
  • 8.1 Abstract
  • 8.2 Introduction
  • 8.2.1 PFG-NMR Principle
  • 8.3 Experimental Details
  • 8.3.1 Materials
  • 8.3.2 Enzyme Activity Assay
  • 8.3.3 NMR and PFG-NMR Experiments
  • 8.4 Results
  • 8.4.1 Diffusion Measurements of Solutions in the Presence of Active Aldolase
  • 8.4.2 Diffusion of the Enzyme Aldolase during Interaction with its InhibitorPyrophosphate
  • 8.4.3 Diffusion of the Enzyme Aldolase during Substrate Conversion
  • 8.5 Discussion
  • 8.6 Conclusion
  • 8.7 Acknowledgments
  • 9 Enzyme Immobilization on Viruses
  • 9.1 Abstract
  • 9.2 Introduction
  • 9.3 Results and Discussion
  • 9.4 Conclusions
  • 9.5 Materials and Methods
  • 9.6 Acknowledgements
  • 10 Misinterpretation of Molecular NMR
  • 11 Conclusions
  • 11.1 Active Diffusion of Enzymes and Chemical Catalysts
  • 11.2 Enzyme-Phage-Colloids
  • 11.3 Outlook
  • A Appendix
  • A.1 Enzyme Diffusion NMR Supplementary Information
  • A.1.1 Aldolase Purification
  • A.1.2 Aldolase Activity Assay
  • A.1.3 Detailed Settings for the PFG-NMR Experiments
  • A.1.4 Detailed Results of the PFG-NMR Experiments
  • A.2 Enzyme-Phage-Colloid Supporting Information
  • A.2.1. Genetic Modification of M13 Bacteriophages Templates
  • A.2.2 Scanning and Transmission Electron Micrographs of Phage-Colloid

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