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20240329122006.0 |
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210213s2021 gw o ||| 0 eng d |
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|a EBLCP
|b eng
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|d OCLCF
|d OCLCO
|d OCLCQ
|d OCLCO
|d OCLCQ
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|a 1236400238
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|a 9783736963641
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|a 3736963645
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|b 000073249543
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|a (OCoLC)1237400868
|z (OCoLC)1236400238
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|a QP601
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|a 612.0151
|2 23
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|a UAMI
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|a Günther, Jan-Philipp.
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|a Advanced Diffusion Studies of Active Enzymes and Nanosystems
|h [electronic resource].
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| 260 |
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|a Göttingen :
|b Cuvillier Verlag,
|c 2021.
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| 300 |
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|a 1 online resource (191 p.)
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| 500 |
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|a Description based upon print version of record.
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|a Intro -- Table of Contents -- Abbreviations -- Abstract -- Kurzzusammenfassung -- 1 Introduction -- 1.1 Publications -- 2 Motion at the Nanoscale -- 2.1 Motion at Low Reynolds Numbers -- 2.2 Brownian Motion -- 3 Active Brownian motion -- 3.1 Self-Phoresis of Micro- and Nanoparticles -- 3.2 Active Diffusion Hypothesis for Enzymes -- 3.3 Molecular Catalysts & Tracer Diffusion -- 4 Diffusion Measurement Techniques -- 4.1 Fluorescence Correlation Spectroscopy -- 4.1.1 Single Molecule Fluorescence -- 4.1.2 Fluorescence Detection from a Confocal volume -- 4.1.3 Autocorrelation Function and Fitting
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| 505 |
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|a 4.2 Nuclear Magnetic Resonance Spectroscopy -- 4.2.1 Nuclear Magnetization -- 4.2.2 Pulsed Field Gradients -- 4.2.3 Advanced Pulse Sequences -- 4.3 Other Techniques -- 4.3.1 Other Correlation Methods -- 4.3.2 Tracking Microscopy -- 4.3.3 Trapping Methods -- 4.3.4 Mutual Diffusion Measurement Techniques -- 4.3.5 Computational Methods -- 5 Enzymes Properties -- 5.1 Alkaline Phosphatase -- 5.2 Fructose-Bisphosphate Aldolase -- 5.3 Urease -- 5.4 FoF1-ATP synthase -- 6 Applications of Enzymes -- 6.1 Biocatalysis -- 6.2 Immobilization -- 6.3 Collective Effects -- 7 Misinterpretation of Enzyme FCS
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| 505 |
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|a 7.1 Conspectus -- 7.2 Introduction -- 7.3 Experiment and Theory of the Enhanced Diffusion of ActiveEnzymes -- 7.4 Fluorescence Correlation Spectroscopy -- 7.5 Enhanced Diffusion of F1-ATPase -- 7.6 Effects and Artefacts in FCS Measurements of ProteinDiffusion -- 7.7 Suggestions for Future FCS Experiments of Enzyme Diffusion -- 7.8 Fluorescence Quenching of Alkaline Phosphatase -- 7.9 Conclusions -- 7.10 Acknowledgment -- 8 Label-Free Enzyme Diffusion Measurements -- 8.1 Abstract -- 8.2 Introduction -- 8.2.1 PFG-NMR Principle -- 8.3 Experimental Details -- 8.3.1 Materials
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|a 8.3.2 Enzyme Activity Assay -- 8.3.3 NMR and PFG-NMR Experiments -- 8.4 Results -- 8.4.1 Diffusion Measurements of Solutions in the Presence of Active Aldolase -- 8.4.2 Diffusion of the Enzyme Aldolase during Interaction with its InhibitorPyrophosphate -- 8.4.3 Diffusion of the Enzyme Aldolase during Substrate Conversion -- 8.5 Discussion -- 8.6 Conclusion -- 8.7 Acknowledgments -- 9 Enzyme Immobilization on Viruses -- 9.1 Abstract -- 9.2 Introduction -- 9.3 Results and Discussion -- 9.4 Conclusions -- 9.5 Materials and Methods -- 9.6 Acknowledgements -- 10 Misinterpretation of Molecular NMR
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|a 11 Conclusions -- 11.1 Active Diffusion of Enzymes and Chemical Catalysts -- 11.2 Enzyme-Phage-Colloids -- 11.3 Outlook -- A Appendix -- A.1 Enzyme Diffusion NMR Supplementary Information -- A.1.1 Aldolase Purification -- A.1.2 Aldolase Activity Assay -- A.1.3 Detailed Settings for the PFG-NMR Experiments -- A.1.4 Detailed Results of the PFG-NMR Experiments -- A.2 Enzyme-Phage-Colloid Supporting Information -- A.2.1. Genetic Modification of M13 Bacteriophages Templates -- A.2.2 Scanning and Transmission Electron Micrographs of Phage-Colloid
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| 500 |
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|a A.2.3 Determination of Enzyme Loading on Phage-Colloids.
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| 504 |
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|a Includes bibliographical references.
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| 590 |
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|a ProQuest Ebook Central
|b Ebook Central Academic Complete
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| 650 |
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|a Enzymes.
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| 650 |
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|a Aldolase.
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| 650 |
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|a Nanotechnology.
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| 650 |
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|a Alkaline phosphatase.
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| 650 |
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2 |
|a Enzymes
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| 650 |
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2 |
|a Alkaline Phosphatase
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| 650 |
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2 |
|a Nanotechnology
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| 650 |
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6 |
|a Enzymes.
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| 650 |
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6 |
|a Phosphatase alcaline.
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| 650 |
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6 |
|a Nanotechnologie.
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| 650 |
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|a enzyme.
|2 aat
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| 650 |
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7 |
|a Aldolase
|2 fast
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| 650 |
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7 |
|a Alkaline phosphatase
|2 fast
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| 650 |
|
7 |
|a Enzymes
|2 fast
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| 650 |
|
7 |
|a Nanotechnology
|2 fast
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| 758 |
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|i has work:
|a Advanced Diffusion Studies of Active Enzymes and Nanosystems (Text)
|1 https://id.oclc.org/worldcat/entity/E39PCFrkqj3yYDG8JR3hgtTd33
|4 https://id.oclc.org/worldcat/ontology/hasWork
|
| 776 |
0 |
8 |
|i Print version:
|a Günther, Jan-Philipp
|t Advanced Diffusion Studies of Active Enzymes and Nanosystems
|d Göttingen : Cuvillier Verlag,c2021
|z 9783736973640
|
| 856 |
4 |
0 |
|u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=6469727
|z Texto completo
|
| 938 |
|
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|a ProQuest Ebook Central
|b EBLB
|n EBL6469727
|
| 938 |
|
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|a YBP Library Services
|b YANK
|n 301919710
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| 938 |
|
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|a EBSCOhost
|b EBSC
|n 2745677
|
| 994 |
|
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|a 92
|b IZTAP
|
