Soil Microbiology
Clasificación: | Libro Electrónico |
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Autor principal: | |
Formato: | Electrónico eBook |
Idioma: | Inglés |
Publicado: |
Newark :
John Wiley & Sons, Incorporated,
2020.
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Edición: | 3rd ed. |
Temas: | |
Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover
- Title Page
- Copyright Page
- Contents
- Preface
- Introduction
- Chapter 1 Soil Ecosystems: Physical and Chemical Boundaries
- 1.1 Soil as an Ecosystem
- 1.1.1 Soil System Function
- 1.1.2 Soil Formation and the Microbial Community
- 1.1.3 Implications of Definition of the Soil Ecosystem
- 1.2 The Micro-ecosystem
- 1.2.1 Interaction of Individual Soil Components with the Biotic System
- 1.2.2 Aboveground and Belowground Communities and Soil Ecosystem Synergistic Development
- 1.3 The Macro-ecosystem
- 1.4 Concluding Comments
- References
- Chapter 2 The Soil Ecosystem: Biological Participants
- 2.1 The Living Soil Component
- 2.1.1 Biological and Genetic Implications of Occurrence of Living Cells in Soil
- 2.1.2 Implications of Microbial Properties for Handling of Soil Samples
- 2.2 Measurement of Soil Microbial Biomass
- 2.2.1 Direct Counting Methods
- 2.2.2 ATP Measure of Soil Microbial Biomass
- 2.2.3 Soil Aerobic Respiration Measurements
- 2.2.4 Chloroform Fumigation (Extraction and Incubation) Technique
- 2.2.5 Limitations of Microbial Biomass Measurements
- 2.3 The Nature of Soil Inhabitants
- 2.4 Autecology and Soil Microbiology
- 2.4.1 Limitations to Autecological Research
- 2.4.2 Autecological Methods
- 2.4.3 PCR for Quantification of Soil Microbes (see Figure 2.7)
- 2.4.4 Expression of Population Density per Unit of Soil
- 2.4.5 Products of Soil Autecological Research
- 2.5 Principles and Products of Synecological Research
- 2.6 Interphase Between Study of Individual and Community Microbiology
- 2.7 Concluding Comments
- References
- Chapter 3 Microbial Diversity of Soil Ecosystems
- 3.1 Classical Culture-Based Studies of Soil Microbial Diversity
- 3.1.1 Value of Culture-Based Studies of Soil Microbial Diversity
- 3.1.2 Limitations of Culture-Based Studies of Soil Microbial Diversity
- 3.1.3 The Challenge of Defining Bacterial Species
- 3.1.4 Alternatives to Bacterial Strain Isolation
- 3.2 Surrogate Measures of Soil Microbial Diversity
- 3.3 Diversity Surrogates: Physiological Profiling
- 3.3.1 Physiological Profiling of Isolates
- 3.3.2 Community-Level Physiological Profiling
- 3.3.3 Value of Community-Level Physiological Profiling
- 3.3.4 Limitations of Community Level Physiological Profiling
- 3.4 Diversity Surrogates: Phospholipid Fatty Acid Analysis
- 3.4.1 PLFA Analysis of Isolates
- 3.4.2 Community PLFA Analysis
- 3.4.3 Value of PLFA Analysis
- 3.4.4 Limitations of PFLA Analysis
- 3.5 Nucleic Acid-Based Analyses of Soil Microbial Diversity
- 3.5.1 Nucleic Acid Based Analysis of Isolates
- 3.5.2 Community Nucleic Acid Analysis
- 3.5.3 DNA Extraction
- 3.5.4 Analysis of Community DNA
- 3.6 PCR-Based Methods
- 3.6.1 Clone Library Sequencing
- 3.6.2 DNA-Based Fingerprinting Techniques
- 3.6.3 High-Throughput Amplicon Sequencing
- 3.6.4 Limitations of PCR-Based Methods