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Preservation of Cells : a Practical Manual.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Newark :
John Wiley & Sons, Incorporated,
2017.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Intro
- Title Page
- Copyright Page
- Contents
- Preface
- Acknowledgments
- Nomenclature
- Chapter 1 Introduction
- Mammalian Cells: Modern Workhorses
- Products from Cells
- Cells as Therapeutic Agents
- Biomarkers for Health or Disease
- In Vitro Models
- Bridging the Gap
- The Preservation Toolkit
- Hypothermic Storage
- Cryopreservation
- Vitrification
- Dry State Storage
- Fit-for-Purpose
- One Size Does Not Fit All
- The Process is the Product
- Reproducibility
- Safety
- Dispelling the Myth of the Cold Black Box
- References
- Chapter 2 Pre-freeze Processing and Characterization
- Pre-freeze Processing
- Digestion of Cells from Intact Tissue
- Hypothermic Storage
- Selection of Subpopulations
- Activation or Stimulation
- Genetic Modification
- Culture
- Pre-freeze Process Monitoring
- Pre-freeze Characterization
- Identity
- Genetic Stability
- Enumeration
- Purity
- Adventitious Agents
- Microbial Testing of Cell Therapy Products
- Special Considerations for the Characterization of Cell Therapies
- Annotation of Pre-freeze Processing
- Scientific Principles
- Putting Principles into Action
- References
- Chapter 3 Formulation and Introduction of Cryopreservation Solutions
- Importance of Cryoprotective Agents
- Mechanisms of Cryoprotection
- Formulating a Cryopreservation Solution
- Formulation of a Vitrification Solution
- Characterization and Quality Control for Cryoprotective Solutions
- Toxicity of CPAs
- Osmotic Toxicity
- Biochemical Toxicity
- Developing a Protocol for Introducing CPA Solutions
- The Basic Experiment
- Introduction of Vitrification Solutions
- Cell Concentration
- Removal of CPA Solution
- Safety Considerations for Cryopreservation Solutions
- Cryopreservation Containers
- Overwraps
- Labeling
- Sample Annotation
- Scientific Principles.
- Putting Principles into Practice
- References
- Chapter 4 Freezing Protocols
- Importance of Cooling Rate
- Controlled-rate Freezing
- Controlled Cooling-rate Protocols
- Segment 1: Initial Hold Period
- Segment 2: Cooling
- Uncontrolled Nucleation
- Manual Nucleation
- Automatic Nucleation
- Verifying Segment 2 (Including S2a)
- "Delayed" Latent Heat
- Segment 3
- Verifying Segment 3
- Other Types of Controlled-rate Protocols
- Passive Freezing
- Transfer to Storage
- Vitrification
- Independent Temperature Measurement
- Scientific Principles
- Putting Principles into Practice
- References
- Chapter 5 Storage and Shipping of Frozen Cells
- Scientific Basis for Selection of a Storage Temperature
- Additional Considerations for Vitrified Samples
- Standards, Guidelines, and Best Practices
- Facilities
- Storage Equipment and Environment
- Mapping Storage Devices and Setting Alarm Limits
- Monitoring Systems
- Safety
- Inventory Management System
- Stability in Storage
- Temperature Fluctuations
- Influence of Background Ionizing Radiation on Stability in Storage
- Shelf-Life of Samples in Storage
- Fit-for-Purpose Storage Practices
- Risk Mitigation in Long-Term Storage
- Shipping or Transport of Cells
- General Shipping Considerations
- Liquid Nitrogen Dry Shippers
- Temperature Mapping of a Shipper
- Packaging of Samples Being Shipped
- Monitoring of Shipments
- Responsibilities
- Sample Annotation
- Scientific Principles
- Putting Principles into Practice
- References
- Chapter 6 Thawing and Post-Thaw Processing
- Thawing Equipment
- Transporting Samples Prior to Thawing
- Estimating Your Thawing Rate
- Thawing and Infusion of Cell Therapy Products
- Safety Considerations for Thawing
- Post-Thaw Processing
- Post-Thaw Washing
- Dilution
- Infusion of Cells Immediately Post-Thaw.
- Removal of Vitrification Solutions
- Wash Solutions
- Scientific Principles
- Putting Principles into Practice
- References
- Chapter 7 Post-Thaw Assessment
- Common Measures Used in Post‐Thaw Assessment
- Physical Integrity
- Metabolic Activity
- Mechanical Activity
- Mitotic Activity
- Differentiation Potential
- Transplantation Potential
- Strategies to Improve the Accuracy and Reproducibility of Post-Thaw Assessment
- Eliminate Measurement Bias
- Compensating for Post-Thaw Apoptosis
- Post-Thaw Assessment Using a Single Measure
- Optical Methods of Post‐Thaw Assessment
- Release Criteria
- Scientific Principles
- Putting Principles into Practice
- References
- Chapter 8 Algorithm-Driven Protocol Optimization
- Small Cell Number/High Throughput Approach
- Validating Operation of the Algorithm
- Flexibility
- Practical Notes
- Modeling in Cryobiology
- References
- Introduction
- ProtocolContributors
- Cryopreservation of Endothelial Cells in Suspension
- Principle
- Equipmentand Supplies
- Equipment
- Supplies
- Safety
- Procedure
- Cell Preparation
- Preparation of Cryoprotectant Solution
- Using Powdered HES
- Using Pentastarch Solution
- Cryoprotectant Addition
- Freezing
- Controlled-rate Freezing with a Methanol Bath
- Alternative Freezing Procedure
- Thawing
- Expected Results
- References
- Cryopreservation of Peripheral Blood Mononuclear Cells from Whole Blood
- Principle
- Protocol 1: Isolation of PBMCS Directly over Ficoll-Hypaque
- Equipment
- Materials
- Reagents
- Procedure
- Protocol 2: Isolation of PBMCS Using SepMates
- Equipment
- Materials
- Reagents
- Procedure
- Materials
- Appendix A Human Serum AB Freezing Media
- Equipment
- Reagents
- Procedure
- Cryopreservation of Human Adipose Stem Cells
- Principle
- Equipmentand Supplies
- Reagentsand Media.
- Procedure
- Isolation of Human ASCs from Lipoaspirate
- Magnetic Cell Sorting (Optional)
- Cryopreservation
- Controlled-rate Freezing of Human ASCs
- Thawing Human ASCs
- Notes
- Reference
- Cryopreservation of Red Blood Cells
- MethodI: High Glycerol/Slow Cooling Technique (Meryman and Hornblower 1972)
- Preparation of the RBC Concentrate
- Addition of the Cryoprotective Solution
- Cooling
- Rewarming
- Removal of the Cryoprotectant and Debris
- MethodII: A Low Glycerol/Rapid Cooling Technique (Rowe, Eyster, and Kellner 1968)
- MethodIII: Hydroxyethylstarch/Rapid Cooling Technique (Sputtek 2007)
- References
- Cryopreservation of Oocytes by Slow Freezing
- Principle
- SpecimenRequirements
- Equipmentand Supplies Needed
- Equipment
- Supplies
- Procedure
- Safety
- Calculations
- Reporting Results
- ProcedureNotes
- Limitationsof Procedure
- Oocyte Vitrification and Warming
- Principle
- Equipmentand Supplies
- Equipment
- Supplies
- Procedure
- QualityControl
- Safety
- Transportation of Hematopoietic Progenitor Cells and Other Cellular Products
- Principle/Rationale
- Specimen
- Equipment/Reagents
- QualityControl
- Procedure
- AdditionalInformation
- FurtherReading
- Cryopreservation of Hematopoietic Progenitor Cells
- Principle/Rationale
- Specimen
- Equipment/Reagents
- QualityControl
- Procedure
- Appendix A Alternate Cryopreservation HarnessSet-2 or 4 Bags
- FurtherReading
- Thawing of Hematopoietic Progenitor Cells
- Principle/Rationale
- Equipment/Reagents
- QualityControl
- Procedure
- FurtherReading
- Processing and Cryopreservation of T-Cells
- Principle/Rationale
- Protocol/Processing Schema: N/A
- Specimens
- Equipment/Reagents
- QualityControl
- Procedure
- FurtherReading
- Thawing and Reinfusion of Cryopreserved T-Cells
- Principle/Rationale.
- Protocol/Processing Schema
- Specimen
- Equipment/Reagents
- QualityControl
- Procedure
- Further Reading
- Index
- EULA.