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Liquid Chromatography : Fundamentals and Instrumentation.
Annotation
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Saint Louis :
Elsevier Science,
2017.
|
| Edición: | 2nd ed. |
| Colección: | Handbooks in Separation Science.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Liquid Chromatography: Fundamentals and Instrumentation; Copyright; Contents; Contributors; Chapter 1: Milestones in the development of liquid chromatography; 1.1 Introduction; 1.1.1 Developments Before 1960; 1.1.2 HPLC at the Beginning; 1.2 HPLC Theory and Practice; 1.2.1 New HPLC Modes and Techniques; 1.2.2 Selection of Conditions for the Control of Selectivity; 1.3 Columns; 1.3.1 Particles and Column Packing; 1.3.2 Stationary Phases and Selectivity; 1.4 Equipment; 1.5 Detectors; Apologies and Acknowledgments; References; Further Reading.
- Chapter 2: Kinetic theories of liquid chromatography2.1 Introduction; 2.2 Macroscopic Kinetic Theories; 2.2.1 Lumped Kinetic Model; 2.2.1.1 van Deemter plate height equation; 2.2.2 General Rate Model; 2.2.2.1 General rate model for monolith columns; 2.2.2.2 General rate model for core-shell particles; 2.2.2.3 Moment analysis; 2.2.3 Lumped Pore Diffusion Model; 2.2.4 Equivalence of the Macroscopic Kinetic Models; 2.2.5 Kinetic Theory of Nonlinear Chromatography; 2.3 Microscopic Kinetic Theories; 2.3.1 Stochastic Model; 2.3.1.1 Stochastic-dispersive model; First passage time.
- 2.3.2 Giddings Plate Height Equation2.3.3 Monte Carlo Simulations of Nonlinear Chromatography; 2.4 Comparison of the Microscopic and the Macroscopic Kinetic Models; References; Further Reading; Chapter 3: Column technology in liquid chromatography; 3.1 Introduction; 3.2 Column Design and Hardware; 3.2.1 Column History in Brief; 3.2.2 Column Hardware; 3.2.3 Column Miniaturization; 3.3 Column Packing Materials and Stationary Phases; 3.3.1 Terminology; 3.3.2 Classification of LC Columns; 3.3.3 Packing Materials [21]; 3.3.3.1 Particle shape, size, and size distribution.
- 3.3.3.2 Pore structure parameters3.3.3.3 Surface functionalization of silica-the key to gaining selectivity; 3.3.3.4 Surface functionalization of silica-the way to bonded silica columns; 3.3.4 Major Synthesis Routes; 3.3.4.1 Physicochemical characterization of bonded silica; 3.3.4.2 Column packing procedures for analytical columns; 3.3.4.3 Examples for selective bonded silica columns; 3.3.4.4 The potential of multimodal or multifunctional bonded columns; 3.4 Column Systems and Operations; 3.4.1 Choice of Average Particle Size and Column Internal Diameter; 3.4.2 Equilibration Time.
- 3.4.3 Choice of Optimum-Flow Conditions3.4.4 Column Back Pressure; 3.4.5 Choice of Column Temperature; 3.4.6 Column Capacity and Loadability; 3.5 Chromatographic Column Testing and Evaluation; 3.5.1 Chromatographic Testing; 3.5.1.1 Hydrophobicity; 3.5.1.2 Silanophilic activity; 3.5.1.3 Polar selectivity; 3.5.1.4 Shape selectivity; 3.5.1.5 Metal content; 3.6 Column Maintenance and Troubleshooting; 3.6.1 Silica-Based Columns; 3.6.1.1 General guidelines; 3.6.2 pH Stability; 3.6.3 Mechanical Stability; 3.6.4 Mobile Phases (Eluents); 3.6.4.1 Proper storage of HPLC columns.