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Small animal imaging : basics and practical guide /
This textbook is a practical guide to the use of small animal imaging in preclinical research that will assist in the choice of imaging modality and contrast agent and in study design, experimental setup, and data evaluation. All established imaging modalities are discussed in detail, with the assis...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cham, Switzerland :
Springer,
[2017]
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| Edición: | Second edition. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Part I: Role of Small Animal Imaging
- Part II: Study Planning and Animal Preparation
- Part III: Imaging Modalities and Probes
- Part IV: Ex Vivo Validation Methods
- Part V: Data Postprocessing
- Part VI: Special Applications
- Appendix: Relevant Physiological and Experimental Rodent Parameters.
- Preface to the Second Edition; Preface to the First Edition; Contents; Abbreviations; Part I: Role of Small Animal Imaging; 1: Noninvasive Imaging for Supporting Basic Research; 1.1 Introduction; 1.2 Imaging Modalities; 1.2.1 Established Modalities; 1.2.2 Newer Modalities; 1.3 Imaging Probes and Contrast Agents; 1.4 Multimodality Imaging; 1.5 Illustrative Applications of Small-Animal Imaging; 1.6 Outlook; References; 2: Non-invasive Imaging in the Pharmaceutical Industry; 2.1 Introduction; 2.2 Target Confidence; 2.3 The Design-Make-Test Cycle; 2.4 Preclinical Pharmacology.
- 2.5 Preclinical Toxicology2.6 Biomarkers; References; 3: Designing a Small Animal Imaging Center; 3.1 Overview; 3.2 Site Requirements; 3.3 Animal Use Issues; 3.4 Anesthesia; 3.5 Radiation Use; 3.6 Biosafety; 3.7 Infrastructure Requirements; 3.7.1 Personnel; 3.7.2 Computer Infrastructure; 3.7.3 Scheduling, Database, and Usage Tracking; 3.8 Ergonomic Flow; 3.9 Training and Education; 3.9.1 Staff Training; 3.9.2 User Education; 3.10 Security; 3.11 Budgeting; 3.12 Emergency Planning; 3.13 Summary; References.
- 4: Noninvasive Small Rodent Imaging: Significance for the 3R Principles4.1 Introduction; 4.2 The Contributions of Imaging to the 3Rs; 4.2.1 Reduction; 4.2.2 Refinement; 4.2.3 Replacement; 4.3 Experimental Lung Research in Small Rodents: Nonimaging Techniques; 4.4 Imaging in Experimental Lung Research: Basic Considerations for Animal Welfare; 4.5 Magnetic Resonance Imaging in Experimental Lung Research: Impact on the 3Rs Leads to More Relevant Data; 4.5.1 Technical Requirements; 4.5.2 Detection and Quantification of the Inflammatory Response: Allergen-Induced Lung Inflammation.
- 4.5.3 Compound Effects Are Detected Earlier Using MRI4.5.4 Structural Changes Are Detectable by MRI: Elastase-ƯInduced Emphysema; 4.5.5 Functional Information Can Be Derived Noninvasively; 4.5.6 Contributions of MRI for the Refinement of Models of Pulmonary Fibrosis in Small Rodents; 4.6 Final Remarks; References; Part II: Study Planning and Animal Preparation; 5: Institutional Preconditions for Small Animal Imaging; 5.1 Background; 5.1.1 Laboratory Animal Facility; 5.2 Functional Areas of the Facility; 5.3 Operative Framework According to Accepted Standards.
- 5.3.1 Temperature and Humidity Control5.3.2 Ventilation and Room Pressurisation; 5.3.3 Power and Lighting; 5.3.4 Noise Control + Vibration; 5.3.5 Animal Husbandry; 5.3.6 Institutional Requirements (Diets, Water, etc.); 5.3.7 Occupational Health and Safety of Personnel; 5.3.8 Animal Transport; 5.4 Classifications of Rodents (Based on Microbiological Status); 5.4.1 General Considerations for an Animal Facility; 5.5 Housing of Animals; Bibliography; 6: Statistical Considerations for Animal Imaging Studies; 6.1 Design of Experiments in Animal Imaging; 6.2 Comparing Interventions.