Cargando…
Fluorescence Microscopy : From Principles to Biological Applications.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Newark :
John Wiley & Sons, Incorporated,
2017.
|
| Edición: | 2nd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Title Page; Copyright; Contents; List of Contributors; Preface; Chapter 1 Introduction to Optics; 1.1 A Short History of Theories about Light; 1.2 Properties of Light Waves; 1.2.1 An Experiment on Interference; 1.2.2 Physical Description of Light Waves; 1.3 Four Effects of Interference; 1.3.1 Diffraction; 1.3.2 The Refractive Index; 1.3.3 Refraction; 1.3.4 Reflection; 1.3.5 Light Waves and Light Rays; 1.4 Optical Elements; 1.4.1 Lenses; 1.4.2 Metallic Mirrors; 1.4.3 Dielectric Mirrors; 1.4.4 Filters; 1.4.5 Chromatic Reflectors; 1.5 Optical Aberrations; References.
- Chapter 2 Principles of Light Microscopy2.1 Introduction; 2.2 Construction of Light Microscopes; 2.2.1 Components of Light Microscopes; 2.2.2 Imaging Path; 2.2.3 Magnification; 2.2.4 Angular and Numerical Aperture; 2.2.5 Field of View; 2.2.6 Illumination Beam Path; 2.2.6.1 Critical and Köhler Illumination; 2.2.6.2 Bright-Field and Epi-Illumination; 2.3 Wave Optics and Resolution; 2.3.1 Wave Optical Description of the Imaging Process; 2.3.2 The Airy Pattern; 2.3.3 Point Spread Function and Optical Transfer Function; 2.3.4 Lateral and Axial Resolution.
- 2.3.4.1 Lateral Resolution Using Incoherent Light Sources2.3.4.2 Lateral Resolution of Coherent Light Sources; 2.3.4.3 Axial Resolution; 2.3.5 Magnification and Resolution; 2.3.6 Depth of Field and Depth of Focus; 2.3.7 Over- and Undersampling; 2.4 Apertures, Pupils, and Telecentricity; 2.5 Microscope Objectives; 2.5.1 Objective Lens Design; 2.5.2 Light Collection Efficiency and Image Brightness; 2.5.3 Objective Lens Classes; 2.5.4 Immersion Media; 2.5.5 Special Applications; 2.6 Contrast; 2.6.1 Dark Field; 2.6.2 Phase Contrast; 2.6.2.1 Frits Zernike's Experiments.
- 2.6.2.2 Setup of a Phase-Contrast Microscope2.6.2.3 Properties of Phase-Contrast Images; 2.6.3 Interference Contrast; 2.6.4 Advanced Topic: Differential Interference Contrast; 2.6.4.1 Optical Setup of a DIC Microscope; 2.6.4.2 Interpretation of DIC Images; 2.6.4.3 Comparison between DIC and Phase Contrast; 2.7 Summary; Acknowledgments; References; Chapter 3 Fluorescence Microscopy; 3.1 Contrast in Optical Microscopy; 3.2 Physical Foundations of Fluorescence; 3.2.1 What is Fluorescence?; 3.2.2 Fluorescence Excitation and Emission Spectra; 3.3 Features of Fluorescence Microscopy.
- 3.3.1 Image Contrast3.3.2 Specificity of Fluorescence Labeling; 3.3.3 Sensitivity of Detection; 3.4 A Fluorescence Microscope; 3.4.1 Principle of Operation; 3.4.2 Sources of Exciting Light; 3.4.3 Optical Filters in a Fluorescence Microscope; 3.4.4 Electronic Filters; 3.4.5 Photodetectors for Fluorescence Microscopy; 3.4.6 CCD or Charge-Coupled Device; 3.4.7 Intensified CCD (ICCD); 3.4.8 Electron-Multiplying Charge-Coupled Device (EMCCD); 3.4.9 CMOS; 3.4.10 Scientific CMOS (sCMOS); 3.4.11 Features of CCD and CMOS Cameras; 3.4.12 Choosing a Digital Camera for Fluorescence Microscopy.