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Forensic Microbiology.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Chicester :
John Wiley & Sons, Incorporated,
2016.
|
| Colección: | Forensic Science in Focus Ser.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Title Page ; Copyright Page; Contents; About the editors; List of contributors; Foreword; Series preface; Preface; Chapter 1 A primer on microbiology; 1.1 Introduction; 1.2 Microbial characteristics; 1.2.1 Microbial taxonomy and function; 1.2.2 Enzyme activity; 1.3 Microorganisms and their habitats; 1.3.1 Oxygen and moisture; 1.3.2 Temperature; 1.4 Competition for resources; 1.5 The ecology of some forensically relevant bacteria; 1.5.1 Actinobacteria; 1.5.2 Firmicutes; 1.5.3 Proteobacteria; 1.6 Archaea and microbial eukaryotes; 1.7 Conclusions; Acknowledgments; References.
- Chapter 2 History, current, and future use of microorganisms as physical evidence2.1 Introduction; 2.1.1 Why and how are microorganisms used in forensic science?; 2.2 Methods for identification; 2.2.1 Classical microbiology; 2.2.2 Genomics and strain typing; 2.3 Estimating PMI; 2.3.1 Microbial succession; 2.4 Cause of death; 2.4.1 Natural causes; 2.4.2 Biocrimes; 2.5 Trace evidence; 2.5.1 Human; 2.5.2 Nonhuman animals and food; 2.6 Other medicolegal aspects; 2.6.1 Sexual assault; 2.6.2 Medical malpractice; 2.6.3 Nosocomial infections and antibiotic resistance.
- 2.6.4 Food safety and environmental contamination2.7 Needs that must be met for use in chain of custody; 2.8 Summary; Acknowledgments; References; Chapter 3 Approaches and considerations for forensic microbiology decomposition research; 3.1 Introduction; 3.2 Challenges of human remains research; 3.3 Human remains research during death investigations; 3.4 Human surrogates in research; 3.5 Considerations for field studies; 3.6 Descriptive and hypothesis-driven research; 3.7 Experiment design; 3.8 Validation studies; Acknowledgments; References; Chapter 4 Sampling methods and data generation.
- 4.1 Introduction4.2 Materials; 4.2.1 Financial considerations; 4.2.2 Terrestrial settings; 4.2.3 Aquatic settings; 4.3 Sample collection techniques; 4.4 Sample preservation, storage, and handling techniques; 4.5 Data considerations; 4.6 Conclusions; Acknowledgments; References; Chapter 5 An introduction to metagenomic data generation, analysis, visualization, and interpretation; 5.1 Introduction; 5.2 DNA extraction; 5.2.1 Sample collection and storage; 5.2.2 Extraction methods; 5.3 DNA sequencing; 5.3.1 Amplicon sequencing of marker (16S rDNA/18S rDNA/ITS) loci.
- 5.3.2 Multi-omics sequencing: metagenomic, metatranscriptomic, metaproteomic, and metametabolomic approaches 5.3.3 Next-generation sequencing platforms ; 5.4 Marker gene data analysis, visualization, and interpretation; 5.4.1 Data analysis pipelines; 5.4.2 Preprocessing of sequence data; 5.4.3 Sequence clustering approaches; 5.4.4 Microbial diversity estimations; 5.5 Multi-omics data analysis, visualization, and interpretation ; 5.5.1 Sequence preprocessing; 5.5.2 Sequence assembly; 5.5.3 Taxonomic profiling; 5.5.4 Gene prediction and metabolic profiling; 5.6 Statistical analysis.