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Identification of the 'Active' Fraction and Metabolic Pathways in Trace Organic Contaminants Removal Using Stable Isotope Probing.
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London :
IWA Publishing,
2016.
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| Colección: | WERF Research Report Series.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Copyright; Acknowledgments; Abstract and Benefits; Table of Contents; List of Tables; List of Figures; List of Acronyms; Executive Summary; Chapter 1.0: Introduction; 1.1 Background; 1.2 Biodegradation of Trace Organic Compounds in Activated Sludge; 1.3 Bisphenol-A as Trace Organic Compound of Interest; 1.4 Tools to Elucidate the 'Active' Fraction and Metabolic Pathways of TOrC Biodegradation in Activated Sludge; 1.5 Organization of Report; Chapter 2.0: DNA-SIP Protocol Optimization; 2.1 Stable Isotope Probing Overview.
- 2.2 Establishment of Optimal Experimental Conditions for DNA-SIP Batch Experiments2.3 Optimization of Separation by Density Gradient Ultracentrifugation; 2.4 Evaluation of DNA Visualization Techniques; Chapter 3.0: Techniques and Methods for BPA Degradation Studies and DNA-SIP Batch Experiments; 3.1 Mixed Culture Nitrifying Community; 3.1.1 Nitrifying Sequencing Batch Reactor; 3.1.2 Evaluation of BPA Biodegradation by Mixed Culture Nitrifying Community; 3.2 Source of Biomass for DNA-SIP Experiments; 3.3 Exposure of Biomass to BPA; 3.3.1 Preliminary Evaluation of BPA Biodegradation.
- 3.3.2 BPA Exposure Conditions3.3.3 Examination of Microbial Community Structure Changes Due to BPA Exposure Using Next Generation Sequencing; 3.3.4 RNA-seq of Microbial Community Exposed to BPA; 3.4 DNA-SIP Experiments; 3.4.1 DNA Extraction and Density Gradient Ultracentrifugation; 3.4.2 Quantification of Gradient Fractions; 3.4.3 Characterization of Gradient Fractions Using Next Generation Sequencing; 3.5 Analytical Methods; Chapter 4.0: Evaluation of BPA Biodegradation by a Mixed Culture Nitrifying Community; 4.1 Nitrifying Sequencing Batch Reactor; 4.2 BPA Biodegradation.
- 6.2 Analysis of SIP Gradient Fractions6.3 Identification of BPA Assimilating Microbes; Chapter 7.0: Conclusions; Appendix A: BPA Biodegradation Metabolite: Time Course Data from DNA-SIP Experiments; References.