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|a GE105.C436 2016
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|a 363.7
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|a UAMI
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|a Chandran, Kartik.
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|a Identification of the 'Active' Fraction and Metabolic Pathways in Trace Organic Contaminants Removal Using Stable Isotope Probing.
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|a London :
|b IWA Publishing,
|c 2016.
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|a 1 online resource (74 pages)
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a WERF Research Report Series ;
|v v.U2R12
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|a Print version record.
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|6 880-01
|a Cover; Copyright; Acknowledgments; Abstract and Benefits; Table of Contents; List of Tables; List of Figures; List of Acronyms; Executive Summary; Chapter 1.0: Introduction; 1.1 Background; 1.2 Biodegradation of Trace Organic Compounds in Activated Sludge; 1.3 Bisphenol-A as Trace Organic Compound of Interest; 1.4 Tools to Elucidate the 'Active' Fraction and Metabolic Pathways of TOrC Biodegradation in Activated Sludge; 1.5 Organization of Report; Chapter 2.0: DNA-SIP Protocol Optimization; 2.1 Stable Isotope Probing Overview.
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|a 2.2 Establishment of Optimal Experimental Conditions for DNA-SIP Batch Experiments2.3 Optimization of Separation by Density Gradient Ultracentrifugation; 2.4 Evaluation of DNA Visualization Techniques; Chapter 3.0: Techniques and Methods for BPA Degradation Studies and DNA-SIP Batch Experiments; 3.1 Mixed Culture Nitrifying Community; 3.1.1 Nitrifying Sequencing Batch Reactor; 3.1.2 Evaluation of BPA Biodegradation by Mixed Culture Nitrifying Community; 3.2 Source of Biomass for DNA-SIP Experiments; 3.3 Exposure of Biomass to BPA; 3.3.1 Preliminary Evaluation of BPA Biodegradation.
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|a 3.3.2 BPA Exposure Conditions3.3.3 Examination of Microbial Community Structure Changes Due to BPA Exposure Using Next Generation Sequencing; 3.3.4 RNA-seq of Microbial Community Exposed to BPA; 3.4 DNA-SIP Experiments; 3.4.1 DNA Extraction and Density Gradient Ultracentrifugation; 3.4.2 Quantification of Gradient Fractions; 3.4.3 Characterization of Gradient Fractions Using Next Generation Sequencing; 3.5 Analytical Methods; Chapter 4.0: Evaluation of BPA Biodegradation by a Mixed Culture Nitrifying Community; 4.1 Nitrifying Sequencing Batch Reactor; 4.2 BPA Biodegradation.
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|a 6.2 Analysis of SIP Gradient Fractions6.3 Identification of BPA Assimilating Microbes; Chapter 7.0: Conclusions; Appendix A: BPA Biodegradation Metabolite: Time Course Data from DNA-SIP Experiments; References.
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|b The widespread presence of trace organic contaminants (TOrC), such as the endocrine disrupting compound bisphenol-A (BPA), has been cause for growing concern due to persistence in the environment and potential ecological impacts. Despite progress made in understanding the removal of TOrC in biological wastewater treatment processes, there is still a lack of consensus regarding the identity of microorganisms active in biodegradation and the exact removal mechanisms involved. This research aimed to develop a clear understanding of the microbial `active' fraction in activated sludge which is responsible for the assimilation of a selected TOrC, BPA. Through the use of DNA stable isotope probing (DNA-SIP), BPA assimilating organisms were identified. This active fraction of BPA assimilation included bacteria related to Sphingobium spp., Sphingomonas spp., Pussilimonas spp., and Variovorax spp. Results from this work also confirm that prior or time-course exposure to BPA as well as bioreactor process conditions influence microbial community structure and function. Added understanding of TOrC removal mechanisms could be derived through continued investigation into microbial functions associated with BPA removal.
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|a Includes bibliographical references.
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| 590 |
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|a ProQuest Ebook Central
|b Ebook Central Academic Complete
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| 650 |
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|a Environmental sciences.
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| 650 |
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|a Economic development
|x Environmental aspects.
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| 650 |
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|a Environmental sciences.
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| 650 |
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|a Sciences de l'environnement.
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|a Développement économique
|x Aspect de l'environnement.
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|a environmental sciences.
|2 aat
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|a Water supply & treatment.
|2 bicssc
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|a Economic development
|x Environmental aspects
|2 fast
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|a Environmental sciences
|2 fast
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|a Environmental science, engineering & technology.
|2 thema
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|a Industrial applications of scientific research & technological innovation.
|2 thema
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|a Mining technology & engineering.
|2 thema
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| 650 |
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|a Water supply & treatment.
|2 thema
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| 650 |
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|a Environment and Ecology.
|2 ukslc
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| 758 |
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|i has work:
|a Identification of the 'Active' Fraction and Metabolic Pathways in Trace Organic Contaminants Removal Using Stable Isotope Probing (Work)
|1 https://id.oclc.org/worldcat/entity/E39PCXmQqBfBwD9q4bwtgrpbbb
|4 https://id.oclc.org/worldcat/ontology/hasWork
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| 776 |
0 |
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|i Print version:
|a Chandran, Kartik.
|t Identification of the 'Active' Fraction and Metabolic Pathways in Trace Organic Contaminants Removal Using Stable Isotope Probing.
|d London : IWA Publishing, ©2016
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| 830 |
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|a WERF Research Report Series.
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| 856 |
4 |
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|u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=4742391
|z Texto completo
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| 880 |
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|6 505-01/(S
|a 4.2.1 BPA Biodegradation Experiments: 35 μg/L BPA Spike4.2.2 BPA Biodegradation Experiments: 900 μg/L BPA Spike; 4.3 Insights Applied to DNA-SIP Experiment Development; Chapter 5.0: BPA Transformation by Microbial Communities from a Full-Scale WWTP; 5.1 Evaluation of BPA Biodegradation; 5.2 BPA Degradation during BPA Exposure; 5.3 Microbial Community Structure Before and After Exposure to BPA; 5.4 Metatranscriptomic Analysis of Microbial Community Exposed to BPA; Chapter 6.0: DNA-SIP Using 13C BPA for Microbial Communities from a Full-Scale WWTP; 6.1 BPA Biodegradation in SIP Experiments.
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