Cargando…
Next Generation Tools for Assessing Death and Decay of Critical Wastewater Bacteria.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London :
IWA Publishing,
2016.
|
| Colección: | WERF Research Report Series.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Copyright; Acknowledgments; Abstract and Benefits; Table of Contents; List of Tables; List of Figures; List of Acronyms; Executive Summary; Chapter 1.0: Introduction; 1.1 Chapter Highlights; 1.2 Background; 1.3 Objectives; 1.4 Traditional and Biomolecular Methods; Chapter 2.0: Insight Into Pathogen and Indicator Inactivation; 2.1 Chapter Highlights; 2.2 Overview; 2.3 Bacterial Strains; 2.3.1 E. coli ECWW; 2.3.2 E. coli K5808; 2.3.3 E. coli SMS3-5; 2.3.4 MRSA; 2.4 Analytical Methods; 2.4.1 TS/VS and pH; 2.4.2 Colony Forming Units (CFUs); 2.4.3 DNA; 2.4.4 RNA.
- 2.4.5 Live/Dead® BacLight"!Bacterial Viability Staining2.4.6 ATP; 2.5 Detection Limits; 2.6 Experimental Design; 2.7 Land Application; 2.7.1 Fate of E. coli during Land Application; 2.7.2 Fate of MRSA during Land Application; 2.7.3 Comparison of cDNA and DNA; 2.8 Mesophilic Digestion; 2.8.1 Fate of E. coli during Mesophilic Digestion; 2.8.2 Fate of MRSA during Mesophilic Digestion; 2.9 Thermophilic Digestion; 2.9.1 Fate of E. coli during Thermophilic Digestion; 2.9.2 Fate of MRSA during Thermophilic Digestion; 2.9.3 Comparison of DNA and cDNA; 2.10 Pasteurization.
- 2.10.1 Fate of E. coli during Pasteurization2.10.2 Fate of MRSA during Pasteurization; 2.10.3 Comparison of DNA and cDNA; 2.11 Conclusions; Chapter 3.0: Qualitative Evaluation of Analytical Methods Used in Pathogen and Pathogen Indicator Inactivation Studies; 3.1 Chapter Highlights; 3.2 Overview; 3.3 TS/VS; 3.4 ATP; 3.5 Live/Dead Staining; 3.6 RT-qPCR (RNA or cDNA); 3.7 DNA; 3.8 CFU; 3.9 Conclusions; Chapter 4.0: Pathogen and Indicator Inactivation Rates; 4.1 Chapter Highlights; 4.2 Microbial Properties and Environmental Factors Affecting Thermal Inactivation Rates.
- 4.3 Mathematical Representations of Inactivation and Decay4.4 Inactivation Rates in Literature; 4.5 Estimating First Order Inactivation Rates; 4.6 Comparison of Inactivation Rates to Literature Values; 4.7 Conclusions; Chapter 5.0: CFU Estimation Tool; 5.1 Chapter Highlights; 5.2 Description of the CFU Estimation Tool; 5.3 Calibration Datasets; 5.4 Calibration Function; 5.5 Model Input and Output; Chapter 6.0: Insight Into Nitrifier Decay; 6.1 Chapter Highlights; 6.2 Overview; 6.3 Nitrifier Decay Experimental Setup; 6.3.1 Master Decay Reactor; 6.3.2 Nitrification Batch Tests.
- 6.4 Analytical Methods6.4.1 Measurement of Ammonia, Nitrite, and Nitrate; 6.4.2 DNA and RNA; 6.4.3 FISH/flow Cytometry; 6.4.4 Illumina Amplicon Sequencing; 6.5 Source Sludge Temperature; 6.6 Removal of Outliers; 6.7 Nitrifier Decay Test Results and Decay Rate Calculations; 6.7.1 AOB and NOB Decay at 14ðC; 6.7.2 AOB and NOB Decay at 17ðC (Run 7); 6.7.3 AOB and NOB Decay at 20ðC (Runs 1, 2, 3, 8, and 10); 6.7.4 AOB and NOB Decay at 25ðC (Run 6); 6.7.5 AOB and NOB Decay at 27ðC, Run 11; 6.7.6 AOB and NOB Decay at 30ðC (Run 4); 6.8 Illumina Amplicon Sequencing.