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EBOOKCENTRAL_ocn963581026 |
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20240329122006.0 |
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161119s2016 enk o 000 0 eng d |
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|a EBLCP
|b eng
|e pn
|c EBLCP
|d OCLCQ
|d IDB
|d OCLCO
|d OCLCF
|d CUY
|d MERUC
|d ZCU
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|a 9781780408194
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|a 1780408196
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|a (OCoLC)963581026
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|a TD735
|b .N498 2016eb
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|a 628.161
|2 23
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|a UAMI
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|a Pruden, Amy.
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|a Next Generation Tools for Assessing Death and Decay of Critical Wastewater Bacteria.
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|a London :
|b IWA Publishing,
|c 2016.
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|a 1 online resource (180 pages)
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a WERF Research Report Series ;
|v v.U1R12
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|a Print version record.
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|a Cover; Copyright; Acknowledgments; Abstract and Benefits; Table of Contents; List of Tables; List of Figures; List of Acronyms; Executive Summary; Chapter 1.0: Introduction; 1.1 Chapter Highlights; 1.2 Background; 1.3 Objectives; 1.4 Traditional and Biomolecular Methods; Chapter 2.0: Insight Into Pathogen and Indicator Inactivation; 2.1 Chapter Highlights; 2.2 Overview; 2.3 Bacterial Strains; 2.3.1 E. coli ECWW; 2.3.2 E. coli K5808; 2.3.3 E. coli SMS3-5; 2.3.4 MRSA; 2.4 Analytical Methods; 2.4.1 TS/VS and pH; 2.4.2 Colony Forming Units (CFUs); 2.4.3 DNA; 2.4.4 RNA.
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|a 2.4.5 Live/Dead® BacLight"!Bacterial Viability Staining2.4.6 ATP; 2.5 Detection Limits; 2.6 Experimental Design; 2.7 Land Application; 2.7.1 Fate of E. coli during Land Application; 2.7.2 Fate of MRSA during Land Application; 2.7.3 Comparison of cDNA and DNA; 2.8 Mesophilic Digestion; 2.8.1 Fate of E. coli during Mesophilic Digestion; 2.8.2 Fate of MRSA during Mesophilic Digestion; 2.9 Thermophilic Digestion; 2.9.1 Fate of E. coli during Thermophilic Digestion; 2.9.2 Fate of MRSA during Thermophilic Digestion; 2.9.3 Comparison of DNA and cDNA; 2.10 Pasteurization.
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|a 2.10.1 Fate of E. coli during Pasteurization2.10.2 Fate of MRSA during Pasteurization; 2.10.3 Comparison of DNA and cDNA; 2.11 Conclusions; Chapter 3.0: Qualitative Evaluation of Analytical Methods Used in Pathogen and Pathogen Indicator Inactivation Studies; 3.1 Chapter Highlights; 3.2 Overview; 3.3 TS/VS; 3.4 ATP; 3.5 Live/Dead Staining; 3.6 RT-qPCR (RNA or cDNA); 3.7 DNA; 3.8 CFU; 3.9 Conclusions; Chapter 4.0: Pathogen and Indicator Inactivation Rates; 4.1 Chapter Highlights; 4.2 Microbial Properties and Environmental Factors Affecting Thermal Inactivation Rates.
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|a 4.3 Mathematical Representations of Inactivation and Decay4.4 Inactivation Rates in Literature; 4.5 Estimating First Order Inactivation Rates; 4.6 Comparison of Inactivation Rates to Literature Values; 4.7 Conclusions; Chapter 5.0: CFU Estimation Tool; 5.1 Chapter Highlights; 5.2 Description of the CFU Estimation Tool; 5.3 Calibration Datasets; 5.4 Calibration Function; 5.5 Model Input and Output; Chapter 6.0: Insight Into Nitrifier Decay; 6.1 Chapter Highlights; 6.2 Overview; 6.3 Nitrifier Decay Experimental Setup; 6.3.1 Master Decay Reactor; 6.3.2 Nitrification Batch Tests.
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|a 6.4 Analytical Methods6.4.1 Measurement of Ammonia, Nitrite, and Nitrate; 6.4.2 DNA and RNA; 6.4.3 FISH/flow Cytometry; 6.4.4 Illumina Amplicon Sequencing; 6.5 Source Sludge Temperature; 6.6 Removal of Outliers; 6.7 Nitrifier Decay Test Results and Decay Rate Calculations; 6.7.1 AOB and NOB Decay at 14ðC; 6.7.2 AOB and NOB Decay at 17ðC (Run 7); 6.7.3 AOB and NOB Decay at 20ðC (Runs 1, 2, 3, 8, and 10); 6.7.4 AOB and NOB Decay at 25ðC (Run 6); 6.7.5 AOB and NOB Decay at 27ðC, Run 11; 6.7.6 AOB and NOB Decay at 30ðC (Run 4); 6.8 Illumina Amplicon Sequencing.
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|a 6.9 Evaluation of Analytical Methods in Determining Nitrifier Decay Rates.
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|b This work used a combination of traditional microbiological and molecular approaches and modeling to advance understanding of decay processes in wastewater treatment. The overall goal was to support improved design and quality control of nitrification and pathogen inactivation processes considerate of more precise estimates of decay parameters. Pathogen and indicator decay experiments were framed with respect to USPEA time-temperature requirements in biosolids. Non-specific molecular methods, such as ATP and live/dead staining, were informative in pure culture but were not suitable as a proxy for pathogen or indicator monitoring in sludge. Existing USEPA time-temperature Class A biosolid regulations are based on CFU; however, DNA persistence suggests that non-cultured microorganisms correspondingly persist. DNA-targeted monitoring holds promise for detection of intact cells in sludge, but more work is necessary to ascertain the viability and infectivity. AOB and NOB measured decay rates were lower than literature values and there was a decline in AOB decay rates at temperatures above 25 DegreesC under the conditions of this study. Reduced decay rates have the most impact under extreme conditions, such as high ammonia loading or low temperatures, where lower decay rates translate to higher active biomass concentrations, lower ammonia peaks, and reduced times without nitrification.
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590 |
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|a ProQuest Ebook Central
|b Ebook Central Academic Complete
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650 |
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0 |
|a Sewage
|x Analysis.
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650 |
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0 |
|a Sewage sludge digestion.
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650 |
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0 |
|a Anaerobic bacteria.
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650 |
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2 |
|a Bacteria, Anaerobic
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650 |
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|a Eaux usées
|x Analyse.
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650 |
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6 |
|a Boues d'épuration
|x Digestion.
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650 |
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6 |
|a Bactéries anaérobies.
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650 |
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7 |
|a Water supply & treatment.
|2 bicssc
|
650 |
|
7 |
|a Anaerobic bacteria
|2 fast
|
650 |
|
7 |
|a Sewage
|x Analysis
|2 fast
|
650 |
|
7 |
|a Sewage sludge digestion
|2 fast
|
650 |
|
7 |
|a Environmental science, engineering & technology.
|2 thema
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650 |
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7 |
|a Industrial applications of scientific research & technological innovation.
|2 thema
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650 |
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7 |
|a Mining technology & engineering.
|2 thema
|
650 |
|
7 |
|a Water supply & treatment.
|2 thema
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650 |
|
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|a Environment and Ecology.
|2 ukslc
|
758 |
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|i has work:
|a Next generation tools for assessing death and decay of critical wastewater bacteria (Text)
|1 https://id.oclc.org/worldcat/entity/E39PCYcvgjmmdRfVwhkbp4hyMK
|4 https://id.oclc.org/worldcat/ontology/hasWork
|
776 |
0 |
8 |
|i Print version:
|a Pruden, Amy.
|t Next Generation Tools for Assessing Death and Decay of Critical Wastewater Bacteria.
|d London : IWA Publishing, ©2016
|
830 |
|
0 |
|a WERF Research Report Series.
|
856 |
4 |
0 |
|u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=4742390
|z Texto completo
|
938 |
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|a Askews and Holts Library Services
|b ASKH
|n AH31622680
|
938 |
|
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|a ProQuest Ebook Central
|b EBLB
|n EBL4742390
|
994 |
|
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|a 92
|b IZTAP
|