Rolling circle amplification : toward new clinical diagnostics and therapeutics /

This book covers the latest developments in rolling circle amplification (RCA) technology with applications in clinical diagnostic tests and molecular medicine. Topics covered include new enzymes useful in RCA, techniques involving RCA for enhanced signal amplification, novel RCA diagnostics, sensor...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Demidov, Vadim V. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cham, Switzerland : Springer, 2016.
Temas:
Gene amplification.
Nucleic acids > Analysis.
Gene Amplification
Life Sciences.
Enzymology.
Biochemical Engineering.
Medical Biochemistry.
Amplification génique.
Acides nucléiques > Analyse.
Biochemical engineering.
Biochemistry.
SCIENCE > Life Sciences > Biochemistry.
Gene amplification
Nucleic acids > Analysis
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Preface
  • Part 1. Improved DNA polymerases and new DNA and DNA/RNA ligases useful in RCA
  • 1. Improvement of?29 DNA polymerase amplification performance by fusion of DNA binding motifs
  • 2. Preparation of circular templates by T4 RNA ligase 2 for rolling circle amplification of target microRNAs with high specificity and sensitivity
  • 3. Use of DNA CircLigase for direct isothermal detection of microbial mRNAs by RNA-primed rolling circle amplification and preparation of ø29 DNA polymerase not contaminated
  • Part 2. RCA-involving techniques with enhanced signal amplification
  • 4. Nicking enzyme-assisted branched-chain RCA reaction for cascade DNA amplification
  • 5. Combining isothermal amplification techniques: Coupled RCA-LAMP
  • Part 3. Emerging RCA diagnostics
  • 6. Detection of vascular disease-related single nucleotide polymorphisms in clinical samples using ramified rolling circle amplification
  • 7. Ultrasensitive isothermal detection of protein analytes using rolling circle amplification in micro-scale platforms
  • 8. Rolling circle amplification with padlock probes for in situ detection of RNA analytes
  • 9. PNA-assisted rolling circle amplification for detection of DNA marker sequences in human cells
  • 10. Sensor systems with magnetic and optomagnetic readout of rolling circle amplification products
  • Part 4. Prospective RCA-based therapeutics
  • 11. DNA nanoclews for stimuli-responsive anticancer drug delivery
  • 12. RCA-assisted self-assembled DNA origami nano-constructs as vehicles for cellular delivery of diagnostic probes and therapeutic drugs
  • 13. DeNAno: a novel multivalent affinity reagent produced by selection of RCA-generated DNA nanoparticle libraries
  • Index.

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