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|a 660.6
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|a UAMI
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|a Joaquim, Cabral,
|e author.
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|a Stem cell manufacturing /
|c Cabral Joaquim, Claudia Lobato da Silva, Maria Fonseca Rodrigues Diogo, Lucas G. Chase.
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| 264 |
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1 |
|a Amsterdam :
|b Elsevier,
|c 2015.
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| 300 |
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|a 1 online resource :
|b illustrations
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| 336 |
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|a text
|b txt
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|a <P>1. Genetic Engineering in Stem Cell Manufacturing<i></p></i> <p>2. Biomechanics in Stem Cell Manufacturing<i></p></i> <p>3. Bioreactor Engineering Fundamentals for Stem Cell Manufacturing<i></p></i> <p>4. Microcarrier Culture Systems for Stem Cell Manufacturing<i></p></i> <p>5. Novel Single-Use Bioreactors for Scale up of Anchorage-Dependent Cell Manufacturing for Cell Therapies<i></p></i> <p>6. Bioreactors and Downstream Processing for Stem Cell Manufacturing<i></p></i> <p>7. Cell production system based on flexible modular platform<i></p></i> <p>8. Microfluidic Devices for the Culture of Stem Cells</p><i> <p></i>9. Enrichment and Separation Technologies for Stem Cell-Based Therapies<i> </p></i> <p>10. Expansion and Characterization Considerations for the Manufacturing of Stem Cells</p><i> <p></i>11. Storage and Delivery of Stem Cells for Cellular Therapies<i></p></i> <p>12. Business Models for Manufacture of Cellular Therapies<i></p></i> <p>13. Stem cells for the regeneration of chronic wounds: a translational perspective<i></p></i>
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|a CIP data; resource not viewed.
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|a Front Cover; STEM CELL MANUFACTURING; STEM CELL MANUFACTURING; Copyright; CONTENTS; LIST OF CONTRIBUTORS; INTRODUCTION; 1 -- Genetic Engineering in Stem Cell Biomanufacturing ; 1.1 INTRODUCTION; 1.2 GENETIC MANIPULATION APPROACHES IN HUMAN PLURIPOTENT STEM CELLS; 1.2.1 Transgenic Approaches; 1.2.2 Knock-In and Knock-Out Approaches; 1.2.2.1 Zinc-Finger Nucleases; 1.2.2.2 Transcription Activator-Like Effector Nucleases; 1.2.2.3 Clustered Regularly Interspaced Short Palindromic Repeat/Cas9; 1.2.3 Bacterial Artificial Chromosomes; 1.3 APPLICATIONS
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|a 1.3.1 Genetic Labeling for Cell Identification and Cell Tracking1.3.2 Gene Alteration for Directed Differentiation; 1.3.3 Gene Disruption for Functional Investigation; 1.3.4 Gene Correction for Function Restoration; 1.4 DELIVERY METHODS; 1.4.1 Transfection; 1.4.2 Nucleofection; 1.4.3 Viral Transduction; 1.5 CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; 2 -- Biomechanics in Stem Cell Manufacturing; 2.1 INTRODUCTION; 2.2 CELLULAR BIOMECHANICS; 2.2.1 Biomechanical Cues; 2.2.2 Shear Forces and Differentiated Cells; 2.2.3 Shear Forces and Pluripotent Stem Cells
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|a 2.3 SCALE UP TOWARD PRODUCTION-LEVEL BIOREACTORS2.4 BIOMANUFACTURING CELLS FOR THERAPIES; 2.4.1 Pluripotent Stem Cells; 2.4.2 Cardiomyocytes; 2.4.3 Endothelial Cells; 2.5 CONCLUSION; REFERENCES; 3 -- Bioreactor Engineering Fundamentals for Stem Cell Manufacturing ; 3.1 INTRODUCTION; 3.2 STIRRED BIOREACTOR BASICS; 3.3 SPECIAL FEATURES OF STIRRED BIOREACTORS FOR HMSC CULTURE ON MICROCARRIERS; 3.3.1 Introduction; 3.3.2 Preparing the Bioreactor for Culture; 3.3.3 Medium and Medium Exchange; 3.3.4 Microcarrier Selection; 3.3.5 Cell and Microcarrier Concentrations; 3.3.6 Attachment Protocol
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|a 3.4.1 Increasing Cell Density3.4.2 Oxygen Demand and Mass Transfer at Higher Cell Density-Sparging and Higher Agitator Speeds; 3.4.3 Carbon Dioxide, Osmolality, and pH; 3.4.4 Human-Induced and Embryonic Pluripotent Stem Cells; 3.5 CONCLUSIONS; NOMENCLATURE; REFERENCES; 4 -- Microcarrier Culture Systems for Stem Cell Manufacturing ; 4.1 OVERVIEW; 4.1.1 Historical Perspective; 4.2 MICROCARRIER TECHNOLOGY; 4.2.1 Types of Microcarriers; 4.2.2 Properties of Microcarriers Required for Cell Culturing; 4.2.3 Advantages of Using Microcarrier Culture Systems for Cell Manufacturing
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| 590 |
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|a ProQuest Ebook Central
|b Ebook Central Academic Complete
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| 650 |
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|a Bioengineering.
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| 650 |
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|a Stem cells
|x Therapeutic use.
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| 650 |
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|a Stem cells
|x Transplantation.
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| 650 |
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|a Biotechnology.
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| 650 |
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|a Biotechnologie.
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| 650 |
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|a Cellules souches
|x Emploi en thérapeutique.
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| 650 |
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|a Cellules souches
|x Greffe.
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| 650 |
|
7 |
|a bioengineering.
|2 aat
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| 650 |
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7 |
|a Biotechnology
|2 fast
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| 650 |
|
7 |
|a Bioengineering
|2 fast
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| 650 |
|
7 |
|a Stem cells
|x Transplantation
|2 fast
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| 700 |
1 |
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|a Silva, Claudia Lobato da,
|e author.
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| 700 |
1 |
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|a Diogo, Maria Fonseca Rodrigues,
|e author.
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| 700 |
1 |
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|a Chase, Lucas G.,
|e author.
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| 758 |
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|i has work:
|a Stem cell manufacturing (Text)
|1 https://id.oclc.org/worldcat/entity/E39PCFFvPjVXWfvx9YC3MWVxj3
|4 https://id.oclc.org/worldcat/ontology/hasWork
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| 776 |
0 |
8 |
|i Print version:
|a Cabral, Joaquim M.S.
|t Stem cell manufacturing.
|d [Place of publication not identified] : Elsevier Science Ltd, 2015
|z 0444632654
|z 9780444632654
|w (OCoLC)949750294
|
| 856 |
4 |
0 |
|u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=4613243
|z Texto completo
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