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Electrophoresis in practice : a guide to methods and applications of DNA and protein separations /

The fifth edition of this successful text has been revised and expanded, omitting some topics on obsolete DNA electrophoresis. It features a section on electrophoretic micro-methods and on-the-chip electrophoresis. It is geared towards advanced students and professionals and contains extended backgr...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Westermeier, Reiner (Autor)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Weinheim, Germany : Wiley-VCH, [2016]
Edición:Fifth edition.
Temas:
Acceso en línea:Texto completo

MARC

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100 1 |a Westermeier, Reiner,  |e author. 
245 1 0 |a Electrophoresis in practice :  |b a guide to methods and applications of DNA and protein separations /  |c Reiner Westermeier. 
250 |a Fifth edition. 
264 1 |a Weinheim, Germany :  |b Wiley-VCH,  |c [2016] 
300 |a 1 online resource (xxvi, 441 pages) :  |b illustrations (some color) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
347 |a data file 
505 0 |a Front Matter -- Part I: Fundamentals. Fundamentals -- Electrophoresis -- Isotachophoresis -- Isoelectric Focusing -- High-Resolution Two-Dimensional Electrophoresis -- Protein Sample Preparation -- Protein Detection -- Blotting -- Part II: Equipment and Methods. Equipment and Methods -- Special Laboratory Equipment -- Consumables -- Chemicals -- Method 1: PAGE of Dyes -- Method 2: Agarose and Immunoelectrophoresis -- Method 3: Titration Curve Analysis -- Method 4: Native PAGE in Amphoteric-Buffers -- Method 5: Agarose IEF -- Method 6: PAGIEF in Rehydrated Gels -- Method 7: Horizontal SDS-PAGE -- Method 8: Vertical PAGE -- Method 9: Semidry Blotting of Proteins -- Method 10: IEF in Immobilized pH Gradients -- Method 11: High-Resolution 2D Electrophoresis -- Method 12: PAGE of DNA Fragments -- Appendix: Troubleshooting. 
504 |a Includes bibliographical references and index. 
588 0 |a Online resource; title from PDF title page (John Wiley, viewed March 7, 2016). 
520 8 |a The fifth edition of this successful text has been revised and expanded, omitting some topics on obsolete DNA electrophoresis. It features a section on electrophoretic micro-methods and on-the-chip electrophoresis. It is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods. 
590 |a ProQuest Ebook Central  |b Ebook Central Academic Complete 
650 0 |a Electrophoresis  |v Laboratory manuals. 
650 0 |a DNA  |x Separation  |v Laboratory manuals. 
650 0 |a Proteins  |x Separation  |v Laboratory manuals. 
650 0 |a Electrophoresis. 
650 2 |a Electrophoresis 
650 6 |a Électrophorèse  |v Manuels de laboratoire. 
650 6 |a ADN  |x Séparation  |v Manuels de laboratoire. 
650 6 |a Protéines  |x Séparation  |v Manuels de laboratoire. 
650 6 |a Électrophorèse. 
650 7 |a SCIENCE  |x Chemistry  |x Physical & Theoretical.  |2 bisacsh 
650 7 |a DNA  |x Separation  |2 fast 
650 7 |a Electrophoresis  |2 fast 
650 7 |a Proteins  |x Separation  |2 fast 
655 2 |a Laboratory Manual 
655 7 |a Laboratory manuals  |2 fast 
655 7 |a Laboratory manuals.  |2 lcgft 
655 7 |a Manuels de laboratoire.  |2 rvmgf 
776 0 8 |i Print version:  |a Westermeier, Reiner.  |t Electrophoresis in practice : a guide to methods and applications of DNA and protein separations.  |b Fifth edition.  |d Weinheim, Germany : Wiley-VCH Verlag, ©2016  |h approximately 532 pages  |z 9783527338801 
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