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Fundamental Bioengineering.

A thorough introduction to the basics of bioengineering, with a focus on applications in the emerging "white" biotechnology industry. As such, this latest volume in the "Advanced Biotechnology" series covers the principles for the design and analysis of industrial bioprocesses as...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Villadsen, John
Formato: Electrónico eBook
Idioma:Inglés
Publicado: [Place of publication not identified] : John Wiley and Sons, Inc., 2015.
Colección:Advanced biotechnology.
Temas:
Acceso en línea:Texto completo

MARC

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490 1 |a Advanced biotechnology 
504 |a Includes bibliographical references and index. 
520 8 |a A thorough introduction to the basics of bioengineering, with a focus on applications in the emerging "white" biotechnology industry. As such, this latest volume in the "Advanced Biotechnology" series covers the principles for the design and analysis of industrial bioprocesses as well as the design of bioremediation systems, and several biomedical applications. No fewer than seven chapters introduce stoichiometry, kinetics, thermodynamics and the design of ideal and real bioreactors, illustrated by more than 50 practical examples. Further chapters deal with the tools that enable an understanding of the behavior of cell cultures and enzymatically catalyzed reactions, while others discuss the analysis of cultures at the level of the cell, as well as structural frameworks for the successful scale-up of bioreactions. In addition, a short survey of downstream processing options and the control of bioreactions is given. With contributions from leading experts in industry and academia, this is a comprehensive source of information peer-reviewed by experts in the field. 
505 0 |a Related Titles; Title Page; Copyright; List of Contributors; About the Series Editors; Chapter 1: Introduction and Overview; Part One: Fundamentals of Bioengineering; Chapter 2: Experimentally Determined Rates of Bio-Reactions; Summary; 2.0 Introduction; 2.1 Mass Balances for a CSTR Operating at Steady State; 2.2 Operation of the Steady-State CSTR; References; Chapter 3: Redox Balances and Consistency Check of Experiments; Summary; 3.1 Black-Box Stoichiometry Obtained in a CSTR Operated at Steady State; 3.2 Calculation of Stoichiometric Coefficients by Means of a Redox Balance. 
505 8 |a 3.3 Applications of the Redox Balance3.4 Composition of the Biomass X; 3.5 Combination of Black-Box Models; 3.6 Application of Carbon and Redox Balances in Bio-Remediation Processes; References; Chapter 4: Primary Metabolic Pathways and Metabolic Flux Analysis; Summary; 4.0 Introduction; 4.1 Glycolysis; 4.2 Fermentative Metabolism: Regenerating the NAD+ Lost in Glycolysis; 4.3 The TCA Cycle: Conversion of Pyruvate to NADH + FADH2, to Precursors or Metabolic Products; 4.4 NADPH and Biomass Precursors Produced in the PP Pathway. 
505 8 |a 4.5 Oxidative Phosphorylation: Production of ATP from NADH (FADH2) in Aerobic Fermentation4.6 Summary of the Biochemistry of Primary Metabolic Pathways; 4.7 Metabolic Flux Analysis Discussed in Terms of Substrate to Product Pathways; 4.8 Metabolic Flux Analysis Discussed in Terms of Individual Pathway Rates in the Network; 4.9 Propagation of Experimental Errors in MFA; 4.10 Conclusions; References; Chapter 5: A Primer to 13C Metabolic Flux Analysis; Summary; 5.1 Introduction; 5.2 Input and Output Data of 13C MFA; 5.3 A Brief History of 13C MFA; 5.4 An Illustrative Toy Example. 
505 8 |a 5.5 The Atom Transition Network5.6 Isotopomers and Isotopomer Fractions; 5.7 The Isotopomer Transition Network; 5.8 Isotopomer Labeling Balances; 5.9 Simulating an Isotope Labeling Experiment; 5.10 Isotopic Steady State; 5.11 Flux Identifiability; 5.12 Measurement Models; 5.13 Statistical Considerations; 5.14 Experimental Design; 5.15 Exchange Fluxes; 5.16 Multidimensional Flux Identifiability; 5.17 Multidimensional Flux Estimation; 5.18 The General Parameter Fitting Procedure; 5.19 Multidimensional Statistics; 5.20 Multidimensional Experimental Design. 
505 8 |a 5.21 The Isotopically Nonstationary Case5.22 Some Final Remarks on Network Specification; 5.23 Algorithms and Software Frameworks for 13C MFA; Glossary; References; Chapter 6: Genome-Scale Models; Summary; 6.1 Introduction; 6.2 Reconstruction Process of Genome-Scale Models; 6.3 Genome-Scale Model Prediction; 6.4 Genome-Scale Models of Prokaryotes; 6.5 Genome-Scale Models of Eukaryotes; 6.6 Integration of Polyomic Data into Genome-Scale Models; Acknowledgment; References; Chapter 7: Kinetics of Bio-Reactions; Summary. 
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