Wildlife DNA Analysis : Applications in Forensic Science.

"This book is the first to approach the fast developing field of wildlife forensics with a focus on the application of DNA profiling and analysis. Case studies throughout link theory and practice and highlight the use of DNA testing in species testing. The text assumes only a basic background k...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Linacre, Adrian
Otros Autores: Tobe, Shanan
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Somerset : John Wiley & Sons, 2013.
Edición:2nd ed.
Colección:Essential Forensic Science.
Temas:
Wildlife crime investigation.
Forensic sciences.
Crimes contre les espèces sauvages > Enquêtes.
Criminalistique.
forensic science.
Forensic sciences
Wildlife crime investigation
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Wildlife DNA Analysis
  • Contents
  • Foreword
  • Preface
  • About the Authors
  • Acknowledgements
  • 1 Introduction
  • 1.1 Importance of wildlife forensic science investigations
  • 1.2 Role of forensic science in wildlife crimes
  • 1.3 Legislation covering wildlife crime
  • 1.4 Role of non-human DNA in forensic science
  • 1.5 Development of wildlife DNA testing
  • 1.5.1 History and current state of wildlife DNA forensic science
  • 1.5.2 Wildlife forensic science testing
  • 1.5.3 Performing DNA typing in wildlife investigations
  • 1.6 Accreditation and certification1.7 Standardisation and validation
  • 1.8 Collection of evidential material, continuity of evidence and transportation to the laboratory
  • 1.9 Note taking and maintenance of a casefile
  • 1.10 Case assessment and initial testing
  • 1.11 Scope of book
  • Useful websites
  • References
  • 2 DNA, Genomes and Genetic Variation
  • 2.1 Introduction
  • 2.2 The DNA molecule
  • 2.3 Chromosomes and nuclear DNA
  • 2.4 Genomes
  • 2.4.1 Nuclear DNA
  • 2.4.2 Mitochondrial and chloroplast DNA
  • 2.5 DNA mutation and genetic variation
  • 2.5.1 Genetic variation of repetitive DNA2.5.2 Single base changes leading to genetic variation
  • 2.5.3 Genetic loci used in species testing
  • 2.6 DNA polymorphisms leading to speciation
  • 2.6.1 Genetic isolation
  • 2.6.2 Other processes leading to speciation
  • 2.7 What is a species?
  • 2.7.1 Subspecies
  • 2.7.2 Genus to Kingdom
  • 2.8 Summary
  • References
  • 3 Methods in Wildlife Forensic DNA Analysis
  • 3.1 Introduction
  • 3.2 Protein polymorphisms
  • 3.3 DNA isolation, purification and concentration
  • 3.3.1 Generic aspects of DNA isolation
  • 3.3.2 Lysis step3.3.3 DNA purification: silica-based extraction
  • 3.3.4 DNA purification: Chelex R 100 resin
  • 3.3.5 DNA purification: organic extraction
  • 3.3.6 Microconcentration
  • 3.4 DNA quantification
  • 3.5 Restriction fragment length polymorphisms (RFLP)
  • 3.6 Methods based on the polymerase chain reaction
  • 3.6.1 Factors affecting PCR efficiency and optimisation of PCR
  • 3.6.2 PCR-based methods of DNA quantification
  • 3.6.3 Random amplification of polymorphic DNA
  • 3.6.4 Amplification of fragment length polymorphisms (AFLP)
  • 3.7 PCR set-up
  • 3.8 PCR clean-up3.9 DNA sequencing
  • 3.10 SNP typing
  • 3.11 New generation of DNA sequence methods
  • Suggested reading
  • 4 Species Testing
  • 4.1 Introduction
  • 4.2 Species
  • 4.2.1 Genetic variation and correspondence with taxonomy
  • 4.3 Attributes of a species testing locus
  • 4.4 Application of a locus to a species
  • 4.5 Tests available and how they are performed
  • 4.5.1 Sequencing
  • 4.5.2 Species-specific primers
  • 4.6 Developing a species test
  • 4.6.1 Use of data on GenBank and sequence alignment
  • 4.6.2 Designing primers

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