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DNA Fingerprinting : an Introduction.
1. Introduction2. Genetic Principles3. Laboratory Organization4. Specimens5. DNA Amplification6. Analysis Techniques7. Probes, Allele Mutations, and Restriction Enzymes8. Probability and Statistical Analysis9. Quality Control10. Legal and Ethical Considerations11. Case Applications.
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Cary :
Oxford University Press, Incorporated,
1993.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- ""CONTENTS""; ""1. INTRODUCTION""; ""DEFINITION""; ""HISTORY""; ""A CASE FOR DNA""; ""APPLICATIONS""; ""REFERENCES""; ""2. GENETIC PRINCIPLES""; ""DNA STRUCTURE""; ""Nuclear DNA""; ""Organelle DNA""; ""DNA FUNCTION""; ""REPRODUCTION""; ""LINKAGE""; ""PEDIGREE ANALYSIS""; ""MUTATION""; ""RESTRICTION ENDONUCLEASES""; ""CONCEPT OF POLYMORPHISM""; ""RECOMBINANT DNA""; ""BASE SEQUENCE DETERMINATION""; ""OLIGONUCLEOTIDE SYNTHESIS""; ""REPETITIVE SEQUENCE ORGANIZATION""; ""NOMENCLATURE OF ARBITRARY DNA SEGMENTS""; ""REFERENCES""; ""3. LABORATORY ORGANIZATION""; ""LABORATORY SECTIONS""; ""Office""
- ""Specimen Documentation""""Specimen Processing""; ""General Preparation""; ""Vector Handling""; ""Amplification by PCR""; ""Hybridization and Radioisotope Handling""; ""Darkroom""; ""Result Interpretation""; ""Tissue Culture""; ""Wash-up Area""; ""EQUIPMENT SUMMARY""; ""OPERATION""; ""Personnel""; ""Work Flow""; ""Safety""; ""REFERENCES""; ""4. SPECIMENS""; ""COLLECTION""; ""STORAGE AND TRANSPORT""; ""DNA EXTRACTION""; ""Principles""; ""Automated System""; ""Manual System Organic Solvent Extraction""; ""Manual System Nonorganic Solvent Extraction""; ""DIALYSIS""; ""RNAASE TREATMENT""
- ""QUANTITATION""""DNA CONCENTRATION""; ""QUALITY DETERMINATION""; ""EFFECTS OF ENVIRONMENTAL FACTORS INCLUDING CONTAMINATING DNA ON DNA PROFILES""; ""ANALYSIS REAGENTS""; ""REFERENCES""; ""5. DNA AMPLIFICATION""; ""POLYMERASE CHAIN REACTION""; ""Amplified Fragment Length Polymorphisms (AMP-FLPs)""; ""Advantages of PCR""; ""Pitfalls in PCR""; ""A PCR Protocol""; ""PROBE AMPLIFICATION""; ""TISSUE CULTURE""; ""REFERENCES""; ""6. ANALYSIS TECHNIQUES""; ""RESTRICTION ENZYME CLEAVAGE""; ""GEL-BLOT ORGANIZATION""; ""Forensic""; ""Parentage""; ""Size Markers""; ""ELECTROPHORESIS""; ""Equipment""
- ""Reagents""""Procedure""; ""PHOTOGRAPHY""; ""DNA RECOVERY""; ""MEMBRANE TRANSFER""; ""Southern Blots""; ""Dot-(Slot- ) Blots""; ""RADIOISOTOPE PROBE LABELING""; ""NONRADIOISOTOPE PROBE LABELING""; ""HYBRIDIZATION""; ""Principles""; ""Procedures""; ""PCR Products""; ""PRINT DETECTION""; ""BLOT STRIPPING AND REPROBING""; ""DATA PROCESSING""; ""Allele Fragment Size Determination""; ""Digital Analysis""; ""The FBI System""; ""Interpretation of Profile Matches""; ""PROFILE REPORT""; ""DNA PROFILE DATA BASES""; ""POTENTIAL PITFALLS""; ""TROUBLESHOOTING GUIDE""; ""ANALYSIS COST""
- ""ANALYSIS REAGENTS""""REFERENCES""; ""7. PROBES, ALLELE MUTATIONS, AND RESTRICTION ENZYMES""; ""PROBES""; ""Polymorphism""; ""Tandem Repeat Single-Locus vs. Multilocus Probes""; ""Oligonucleotide Probes""; ""Amplification and Stability""; ""Availability""; ""Probes Used in Forensic Analysis""; ""MUTATIONS""; ""Allele Formation""; ""Genotype Analysis""; ""RESTRICTION ENZYMES""; ""Recognition Site Features""; ""Fragment Resolution""; ""Sensitivity to Inhibitors and Stability""; ""Price and Availability""; ""The Enzymes Selected for Forensic Analysis""; ""REFERENCES""