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DNA Fingerprinting : an Introduction.

1. Introduction2. Genetic Principles3. Laboratory Organization4. Specimens5. DNA Amplification6. Analysis Techniques7. Probes, Allele Mutations, and Restriction Enzymes8. Probability and Statistical Analysis9. Quality Control10. Legal and Ethical Considerations11. Case Applications.

Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Kirby, Lorne T.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Cary : Oxford University Press, Incorporated, 1993.
Temas:
Acceso en línea:Texto completo

MARC

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245 1 0 |a DNA Fingerprinting :  |b an Introduction. 
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505 0 |a ""CONTENTS""; ""1. INTRODUCTION""; ""DEFINITION""; ""HISTORY""; ""A CASE FOR DNA""; ""APPLICATIONS""; ""REFERENCES""; ""2. GENETIC PRINCIPLES""; ""DNA STRUCTURE""; ""Nuclear DNA""; ""Organelle DNA""; ""DNA FUNCTION""; ""REPRODUCTION""; ""LINKAGE""; ""PEDIGREE ANALYSIS""; ""MUTATION""; ""RESTRICTION ENDONUCLEASES""; ""CONCEPT OF POLYMORPHISM""; ""RECOMBINANT DNA""; ""BASE SEQUENCE DETERMINATION""; ""OLIGONUCLEOTIDE SYNTHESIS""; ""REPETITIVE SEQUENCE ORGANIZATION""; ""NOMENCLATURE OF ARBITRARY DNA SEGMENTS""; ""REFERENCES""; ""3. LABORATORY ORGANIZATION""; ""LABORATORY SECTIONS""; ""Office"" 
505 8 |a ""Specimen Documentation""""Specimen Processing""; ""General Preparation""; ""Vector Handling""; ""Amplification by PCR""; ""Hybridization and Radioisotope Handling""; ""Darkroom""; ""Result Interpretation""; ""Tissue Culture""; ""Wash-up Area""; ""EQUIPMENT SUMMARY""; ""OPERATION""; ""Personnel""; ""Work Flow""; ""Safety""; ""REFERENCES""; ""4. SPECIMENS""; ""COLLECTION""; ""STORAGE AND TRANSPORT""; ""DNA EXTRACTION""; ""Principles""; ""Automated System""; ""Manual System Organic Solvent Extraction""; ""Manual System Nonorganic Solvent Extraction""; ""DIALYSIS""; ""RNAASE TREATMENT"" 
505 8 |a ""QUANTITATION""""DNA CONCENTRATION""; ""QUALITY DETERMINATION""; ""EFFECTS OF ENVIRONMENTAL FACTORS INCLUDING CONTAMINATING DNA ON DNA PROFILES""; ""ANALYSIS REAGENTS""; ""REFERENCES""; ""5. DNA AMPLIFICATION""; ""POLYMERASE CHAIN REACTION""; ""Amplified Fragment Length Polymorphisms (AMP-FLPs)""; ""Advantages of PCR""; ""Pitfalls in PCR""; ""A PCR Protocol""; ""PROBE AMPLIFICATION""; ""TISSUE CULTURE""; ""REFERENCES""; ""6. ANALYSIS TECHNIQUES""; ""RESTRICTION ENZYME CLEAVAGE""; ""GEL-BLOT ORGANIZATION""; ""Forensic""; ""Parentage""; ""Size Markers""; ""ELECTROPHORESIS""; ""Equipment"" 
505 8 |a ""Reagents""""Procedure""; ""PHOTOGRAPHY""; ""DNA RECOVERY""; ""MEMBRANE TRANSFER""; ""Southern Blots""; ""Dot-(Slot- ) Blots""; ""RADIOISOTOPE PROBE LABELING""; ""NONRADIOISOTOPE PROBE LABELING""; ""HYBRIDIZATION""; ""Principles""; ""Procedures""; ""PCR Products""; ""PRINT DETECTION""; ""BLOT STRIPPING AND REPROBING""; ""DATA PROCESSING""; ""Allele Fragment Size Determination""; ""Digital Analysis""; ""The FBI System""; ""Interpretation of Profile Matches""; ""PROFILE REPORT""; ""DNA PROFILE DATA BASES""; ""POTENTIAL PITFALLS""; ""TROUBLESHOOTING GUIDE""; ""ANALYSIS COST"" 
505 8 |a ""ANALYSIS REAGENTS""""REFERENCES""; ""7. PROBES, ALLELE MUTATIONS, AND RESTRICTION ENZYMES""; ""PROBES""; ""Polymorphism""; ""Tandem Repeat Single-Locus vs. Multilocus Probes""; ""Oligonucleotide Probes""; ""Amplification and Stability""; ""Availability""; ""Probes Used in Forensic Analysis""; ""MUTATIONS""; ""Allele Formation""; ""Genotype Analysis""; ""RESTRICTION ENZYMES""; ""Recognition Site Features""; ""Fragment Resolution""; ""Sensitivity to Inhibitors and Stability""; ""Price and Availability""; ""The Enzymes Selected for Forensic Analysis""; ""REFERENCES"" 
500 |a ""8. probability and statistical analysis"" 
520 |a 1. Introduction2. Genetic Principles3. Laboratory Organization4. Specimens5. DNA Amplification6. Analysis Techniques7. Probes, Allele Mutations, and Restriction Enzymes8. Probability and Statistical Analysis9. Quality Control10. Legal and Ethical Considerations11. Case Applications. 
590 |a ProQuest Ebook Central  |b Ebook Central Academic Complete 
650 0 |a DNA fingerprinting. 
650 0 |a Forensic genetics  |x Technique. 
650 2 |a DNA Fingerprinting 
650 6 |a Empreintes génétiques. 
650 6 |a Génétique légale  |x Technique. 
650 7 |a DNA fingerprinting  |2 fast 
650 7 |a Forensic genetics  |x Technique  |2 fast 
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776 0 8 |i Print version:  |a Kirby, Lorne T.  |t DNA Fingerprinting : An Introduction.  |d Cary : Oxford University Press, Incorporated, ©1993  |z 9780195118674 
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