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Plant transcription factors : evolutionary, structural and functional sspects /

Plant Transcription Factors: Evolutionary, Structural and Functional Aspects is the only publication that provides a comprehensive compilation of plant transcription factor families and their complex roles in plant biology. While the majority of information about transcription factors is based on ma...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Gonzalez, Daniel H. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: London : Academic Press, an imprint of Elsevier, 2015.
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Cover; Title page; Copyright Page; Contents; List of Contributors; Preface; Section A
  • General Aspects of Plant Transcription Factors; Chapter 1
  • Introduction to Transcription Factor Structure and Function; 1.1
  • Introduction: Transcription in eukaryotes; 1.2
  • Structure of transcription factors; 1.3
  • DNA recognition by transcription factors; 1.4
  • DNA-binding domains; 1.5
  • Protein-protein interactions; 1.6
  • Regulation of transcription factor action; 1.7
  • Plant transcription factors; Acknowledgments; References; Chapter 2
  • Methods to Study Transcription Factor Structure and Function.
  • 2.1
  • Introduction2.2
  • In vivo functional studies; 2.2.1
  • Overexpression or Ectopic Expression of Transcription Factors; 2.2.2
  • Transactivation Systems for Expression of Transcription Factors; 2.2.3
  • Expression of Dominant Negative Forms of Transcription Factors; 2.2.4
  • Expression of Dysregulated Forms of Transcription Factors; 2.2.5
  • Gain-of-Function Mutants; 2.2.6
  • Expression of Fusions to Activating or Repressor Domains; 2.2.7
  • Inducible Systems; 2.3
  • Methods for the analysis of in vitro protein-DNA interactions; 2.3.1
  • The EMSA Assay; 2.3.2
  • Selex.
  • 2.3.3
  • Analysis of Transcription Factor-DNA Complexes by Footprinting Assays2.3.4
  • Microarray-Based Identification of Transcription Factor Target Genes; 2.4
  • Methods to study protein-DNA interactions in vivo; 2.4.1
  • Chromatin Immunoprecipitation (ChIP) Assays; 2.4.2
  • ChIP-chip and ChIP-Seq; 2.4.3
  • DNA Adenine Methyltransferase Identification (DamID); 2.4.4
  • Identification of Transcription Factors Using the Yeast One-Hybrid Assay; 2.4.5
  • Transient Assays to Analyze Protein-DNA Interactions In Vivo; 2.5
  • Analysis of protein-protein interactions.
  • 2.5.1
  • Methods for Protein-Protein Complex Identification2.5.1.1
  • Yeast Two-Hybrid Assay (Y2H); 2.5.1.2
  • Tandem and One-step Tag-Based Affinity Purification; 2.5.2
  • Methods for Verification of Protein-Protein Interactions; 2.5.2.1
  • Coimmunoprecipitation; 2.5.2.2
  • In Vivo Split Methods; 2.5.2.3
  • Resonance Energy Transfer Methods; Acknowledgments; References; Chapter 3
  • General Aspects of Plant Transcription Factor Families; 3.1
  • Introduction; 3.2
  • Overview of the transcription cycle in eukaryotes; 3.2.1
  • Core Promoter Elements and General Transcription Factors.
  • 3.2.2
  • Role of Nucleosome-Modifying Enzymes and Chromatin Remodelers3.2.3
  • Productive Transcription Initiation and Elongation; 3.2.4
  • Transcription, Termination, and Reinitiation; 3.3
  • Components involved in the formation of the RNAPII preinitiation complex in plants; 3.3.1
  • GTFs and RNAPII; 3.3.2
  • Mediator; 3.3.3
  • Corepressors of the Plant Gro/Tup1 Family; 3.4
  • Plant transcription factor families; 3.5
  • Major TF families that are conserved across eukaryotes; 3.5.1
  • bZIP Transcription Factor Family; 3.5.2
  • bHLH TF Family; 3.5.3
  • MYB TF Family; 3.5.4
  • HSF Family.
  • 3.6
  • Plant-specific TF families.