Plant transcription factors : evolutionary, structural and functional sspects /
Plant Transcription Factors: Evolutionary, Structural and Functional Aspects is the only publication that provides a comprehensive compilation of plant transcription factor families and their complex roles in plant biology. While the majority of information about transcription factors is based on ma...
Clasificación: | Libro Electrónico |
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Otros Autores: | |
Formato: | Electrónico eBook |
Idioma: | Inglés |
Publicado: |
London :
Academic Press, an imprint of Elsevier,
2015.
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Temas: | |
Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Title page; Copyright Page; Contents; List of Contributors; Preface; Section A
- General Aspects of Plant Transcription Factors; Chapter 1
- Introduction to Transcription Factor Structure and Function; 1.1
- Introduction: Transcription in eukaryotes; 1.2
- Structure of transcription factors; 1.3
- DNA recognition by transcription factors; 1.4
- DNA-binding domains; 1.5
- Protein-protein interactions; 1.6
- Regulation of transcription factor action; 1.7
- Plant transcription factors; Acknowledgments; References; Chapter 2
- Methods to Study Transcription Factor Structure and Function.
- 2.1
- Introduction2.2
- In vivo functional studies; 2.2.1
- Overexpression or Ectopic Expression of Transcription Factors; 2.2.2
- Transactivation Systems for Expression of Transcription Factors; 2.2.3
- Expression of Dominant Negative Forms of Transcription Factors; 2.2.4
- Expression of Dysregulated Forms of Transcription Factors; 2.2.5
- Gain-of-Function Mutants; 2.2.6
- Expression of Fusions to Activating or Repressor Domains; 2.2.7
- Inducible Systems; 2.3
- Methods for the analysis of in vitro protein-DNA interactions; 2.3.1
- The EMSA Assay; 2.3.2
- Selex.
- 2.3.3
- Analysis of Transcription Factor-DNA Complexes by Footprinting Assays2.3.4
- Microarray-Based Identification of Transcription Factor Target Genes; 2.4
- Methods to study protein-DNA interactions in vivo; 2.4.1
- Chromatin Immunoprecipitation (ChIP) Assays; 2.4.2
- ChIP-chip and ChIP-Seq; 2.4.3
- DNA Adenine Methyltransferase Identification (DamID); 2.4.4
- Identification of Transcription Factors Using the Yeast One-Hybrid Assay; 2.4.5
- Transient Assays to Analyze Protein-DNA Interactions In Vivo; 2.5
- Analysis of protein-protein interactions.
- 2.5.1
- Methods for Protein-Protein Complex Identification2.5.1.1
- Yeast Two-Hybrid Assay (Y2H); 2.5.1.2
- Tandem and One-step Tag-Based Affinity Purification; 2.5.2
- Methods for Verification of Protein-Protein Interactions; 2.5.2.1
- Coimmunoprecipitation; 2.5.2.2
- In Vivo Split Methods; 2.5.2.3
- Resonance Energy Transfer Methods; Acknowledgments; References; Chapter 3
- General Aspects of Plant Transcription Factor Families; 3.1
- Introduction; 3.2
- Overview of the transcription cycle in eukaryotes; 3.2.1
- Core Promoter Elements and General Transcription Factors.
- 3.2.2
- Role of Nucleosome-Modifying Enzymes and Chromatin Remodelers3.2.3
- Productive Transcription Initiation and Elongation; 3.2.4
- Transcription, Termination, and Reinitiation; 3.3
- Components involved in the formation of the RNAPII preinitiation complex in plants; 3.3.1
- GTFs and RNAPII; 3.3.2
- Mediator; 3.3.3
- Corepressors of the Plant Gro/Tup1 Family; 3.4
- Plant transcription factor families; 3.5
- Major TF families that are conserved across eukaryotes; 3.5.1
- bZIP Transcription Factor Family; 3.5.2
- bHLH TF Family; 3.5.3
- MYB TF Family; 3.5.4
- HSF Family.
- 3.6
- Plant-specific TF families.