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Photonics. scientific foundations, technology, and applications / Biomedical photonics, spectroscopy, and microscopy. Volume IV :
Discusses the basic physical principles underlying Biomedical Photonics, spectroscopy and microscopy This volume discusses biomedical photonics, spectroscopy and microscopy, the basic physical principles underlying the technology and its applications. The topics discussed in this volume are: Biophot...
| Clasificación: | Libro Electrónico |
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| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Hoboken, New Jersey :
Wiley,
2015.
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| Colección: | A Wiley-Science Wise Co-Publication.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Photonics; Contents; List of Contributors; Preface; 1 Fluorescence; 1.1 Introduction; 1.2 Spectra; 1.2.1 Background and Theory; 1.2.2 Experimental; 1.2.3 Application Example-Melanin Spectra; 1.3 Quantum Yield; 1.3.1 Theory; 1.3.2 Experimental; 1.3.3 Application Example-ThT Detection of Sheet Structure; 1.4 Lifetime; 1.4.1 Theory; 1.4.2 Experimental; 1.4.3 Application Example-In Vivo Glucose Sensing; 1.5 Quenching; 1.5.1 Theory; 1.5.2 Application-Metal Ion Quenching; 1.6 Anisotropy; 1.6.1 Theory; 1.6.2 Experimental; 1.6.3 Application Example-Nanoparticle Metrology; 1.7 Microscopy.
- 1.7.1 Systems and Techniques1.7.2 Application Example-Gold Nanorods in Cells; 1.8 Conclusions; Acknowledgments; 2 Single-Molecule Detection and Spectroscopy; 2.1 Introduction; 2.2 Experimental Setups; 2.2.1 Principles; 2.2.2 Correction of Aberrations; 2.2.3 Polarization Structure at the Focus; 2.2.4 Various Microscopy Methods; 2.3 Fluorescence Spectroscopy; 2.3.1 Introduction, Signal-to-Noise Ratio; 2.3.2 Sample Preparation; 2.3.3 Orientation; 2.3.4 Blinking; 2.3.5 Bleaching; 2.3.6 Superresolution; 2.4 Fluorescence Correlation Spectroscopy; 2.4.1 Photon Counting Histograms, Burst Analysis.
- 2.4.2 Fluorescence Correlation Spectroscopy2.4.3 Multiparameter Analysis; 2.5 Fluorescence Excitation Spectroscopy; 2.5.1 Zero-Phonon Line and Phonon Wing; 2.5.2 Inhomogeneous Broadening; 2.5.3 Hole-Burning [30]; 2.5.4 Single-Molecule Spectroscopy; 2.5.5 Scope of Low-Temperature Single-Molecule Spectroscopy; 2.6 Other Detection Methods; 2.6.1 General Considerations on Signal, Background, and Noise; 2.6.2 Dark-Field Scattering, Total Internal Reflection; 2.6.3 Absorption, Extinction, Interference-Based Methods; 2.6.4 Pump-Probe and Photothermal Detections; 2.7 Conclusion; Acknowledgments.
- 3.4.3 Intensity-Based Imaging3.4.4 Lifetime-Based Imaging; 3.4.5 Other Applications; 3.5 The Role of RET in Light-Harvesting Complexes; 3.5.1 Introduction; 3.5.2 Photosynthetic Excitons; Acknowledgments; References; 4 Biophotonics of Photosynthesis; 4.1 Introduction; 4.2 Structure of Pigment-Protein Complexes and Structure-Function Relationships; 4.2.1 Photosystem I (PS I) and Photosystem II (PS II); 4.2.2 PCs of Purple Bacteria; 4.3 Key Concepts in Physics of Pigment-Protein Complexes; 4.3.1 Excitons; 4.3.2 Excitation Energy Transfer.