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Epigenetics and Dermatology.
Epigenetics and Dermatology explores the role of epigenetics in the pathogenesis of autoimmune-related skin diseases and skin cancer. Leading contributors cover common and uncommon skin conditions in which extensive epigenetic research has been done. They explain how environmental exposures (chemica...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Elsevier Science,
2015.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Epigenetics and Dermatology; Copyright Page; Dedication; Contents; List of Contributors; Preface; Acknowledgments; 1. Biological and Historical Aspects of Epigenetics; 1 Introduction to Epigenetics; References; 2 Laboratory Methods in Epigenetics; 2.1 Introduction; 2.2 DNA Methylation Analysis; 2.2.1 Methods to Distinguish 5-Methylcytosine from Cytosine; 2.2.1.1 Restriction Endonuclease-Based Analysis; 2.2.1.1.1 Southern Blot; 2.2.1.1.2 Methylation-Sensitive Amplified Polymorphism; 2.2.1.2 Bisulfite Conversion Technique and Derivatives; 2.2.1.2.1 Bisulfite Sequencing PCR
- 2.2.1.2.2 Pyrosequencing2.2.1.2.3 Combined Bisulfite and Restriction Analysis; 2.2.1.2.4 Methylation-Sensitive Single-Nucleotide Primer Extension and SnuPE Ion Pair Reversed-Phase High Performance Liqui...; 2.2.1.2.5 Methylation-Sensitive Melting Curve Analysis; 2.2.1.2.6 Methylation-Sensitive High-Resolution Melting; 2.2.1.2.7 MethyLight; 2.2.1.3 Immunoprecipitation-Based Methods; 2.2.1.3.1 Methylated-CpG Island Recovery Assay; 2.2.1.3.2 Methyl-Binding-PCR; 2.2.1.4 Mass Spectrometry-Based Methods; 2.2.1.4.1 MALDI-TOF Mass Spectrometry with Base-Specific Cleavage
- 2.2.1.4.2 MALDI-TOF Mass Spectrometry with Primer Extension2.2.2 Genome-Scale DNA Methylation Analysis; 2.2.2.1 Microarray-Based Analysis of DNA Methylation Changes; 2.2.2.1.1 Sample Preparation; 2.2.2.1.2 Microarray Used in DNA Methylation Profiling; 2.2.2.2 Next-Generation Sequencing Techniques; 2.3 Techniques Used for 5hmC Mark Detection; 2.4 Histone Modification Analysis; 2.4.1 Chromatin Immunoprecipitation; 2.4.2 ChIP-on-Chip; 2.4.3 ChIP-seq; 2.4.3.1 Workflow of ChIP-seq; 2.4.3.2 Analysis Pipeline of ChIP-seq Data; 2.4.3.2.1 Read Aligner; 2.4.3.2.2 Peak Calling; 2.4.3.2.3 Motif Finding
- 2.4.3.3 Advantages of ChIP-seq2.4.4 Challenges for Histone Modification Analysis; 2.5 miRNA Analysis; 2.5.1 miRNA Detection; 2.5.1.1 Microarray; 2.5.1.2 Next-Generation Sequencing; 2.5.1.3 RT-PCR; 2.5.1.4 Northern Blot Analysis; 2.5.1.5 Others; 2.5.2 Target Prediction; 2.5.2.1 Target Scan; 2.5.2.2 PicTar; 2.5.2.3 DIANA-microT; 2.5.2.4 Others; 2.5.3 Target Validation and Functional Analysis; 2.5.3.1 Luciferase Reporter Assays; 2.5.3.2 Gain-of-Function and Loss-of-Function Experiments; 2.6 Conclusion; List of Abbreviations; References; 3 Keratinocyte Differentiation and Epigenetics
- 3.1 Introduction3.2 Gene Expression in Keratinocyte Differentiation; 3.3 Epigenetic Modulation in Keratinocyte Differentiation; 3.4 Epigenetics and Skin Diseases; 3.5 Conclusion; References; 4 Epigenetics and Fibrosis: Lessons, Challenges, and Windows of Opportunity; 4.1 Introduction; 4.2 Incidence and Prevalence of Fibrosis; 4.3 Biology of Epigenetics; 4.3.1 DNA Methylation; 4.3.2 Histone Modifications; 4.4 Epigenetics and Fibrosis; 4.4.1 Epigenetics and Lung Fibrosis; 4.4.1.1 DNA Methylation and Lung Fibrosis; 4.4.1.2 Histone Modifications and Lung Fibrosis