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Analyzing biomolecular interactions by mass spectrometry /
This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration,...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Weinheim [Germany] :
Wiley-VCH,
[2015]
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Analyzing Biomolecular Interactions by Mass Spectrometry
- Contents
- List of Contributors
- Preface
- Abbreviations
- Chapter 1 Introduction to Mass Spectrometry, a Tutorial
- 1.1 Introduction
- 1.2 Figures of Merit
- 1.2.1 Introduction
- 1.2.2 Resolution
- 1.2.3 Mass Accuracy
- 1.2.4 General Data Acquisition in MS 1.3 Analyte Ionization
- 1.3.1 Introduction
- 1.3.2 Electrospray Ionization
- 1.3.3 Matrix-Assisted Laser Desorption Ionization
- 1.3.4 Other Ionization Methods
- 1.3.5 Solvent and Sample Compatibility Issues
- 1.4 Mass Analyzer Building Blocks 1.4.1 Introduction
- 1.4.2 Quadrupole Mass Analyzer
- 1.4.3 Ion-Trap Mass Analyzer
- 1.4.4 Time-of-Flight Mass Analyzer
- 1.4.5 Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
- 1.4.6 Orbitrap Mass Analyzer
- 1.4.7 Ion Detection 1.5 Tandem Mass Spectrometry
- 1.5.1 Introduction: Tandem-in-Time and Tandem-in-Space
- 1.5.2 Ion Dissociation Techniques
- 1.5.3 Tandem Quadrupole MS-MS Instruments
- 1.5.4 Ion-Trap MS n Instruments
- 1.5.5 Tandem TOF (TOF-TOF) Instruments
- 1.5.6 Hybrid Instruments (Q-TOF, Q-LIT, IT-TOF) 1.5.7 MS-MS and MSn in FT-ICR-MS
- 1.5.8 Orbitrap-Based Hybrid Systems
- 1.5.9 Ion-Mobility Spectrometry-Mass Spectrometry
- 1.6 Data Interpretation and Analytical Strategies
- 1.6.1 Data Acquisition in MS Revisited