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Omics in Plant Breeding.
The use of computational and high-throughput methods, such as genomics, proteomics, transcriptomics, or known collectively as "--Omics", to study plant biology for well over a decade now. As these technologies mature, plant and crop scientists have started using these methods for improving...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Hoboken :
Wiley,
2014.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Title Page; Copyright; Contents; List of Contributors; Foreword; Chapter 1 Omics: Opening up the ""Black Box"" of the Phenotype; The Post-Genomics Era; The Omics in Plant Breeding; Genomics, Precision Genomics, and RNA Interference; Transcriptomics and Proteomics; Metabolomics and Physiognomics; Phenomics; Bioinformatics; Prospects; References; Chapter 2 Genomics; The Rise of Genomics; DNA Sequencing; Development of Sequence-based Markers; Genetic Mapping; Association Mapping; Genome Wide Selection (GWS); Comparative Genomics; Structural and Comparative Genomics.
- Applications of Genomics in Plant BreedingReferences; Chapter 3 Transcriptomics; Methods of Studying the Transcriptome; Construction of cDNA Libraries for EST Sequencing; Suppression Subtractive Hybridization (SSH); Serial Analysis of Gene Expression (SAGE); Microarrays; RNA-seq; Applications of Transcriptomics Approaches for Crop Breeding; Conclusions and Future Prospects; Acknowledgements; References; Chapter 4 Proteomics; History; Different Methods for the Extraction of Total Proteins; Importance of High-quality Protein Extracts; Extraction with TCA/Acetone; Phenolic Extraction.
- Extraction with Phenol and Precipitation with TCA/AcetoneProtein Extraction: Removal of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO); Subcellular Proteomics; Post-Translational Modifications; Phosphorylation; Quantitative Proteomics; Quantification with Isotope Labeling of Proteins and Peptides; Label-free Quantification; Perspectives; References; Chapter 5 Metabolomics; Introduction; Metabolomic and Biochemical Molecules; Technologies for Metabolomics; Metabolomic Database Analysis; Metabolomics Applications; Metabolomics-assisted Plant Breeding.
- Plant Breeding for Quality Metabolic CompoundsMetabolites QTLs (mQTLs) Expression; Simple Studies on the Yield Components; Studies of Multiple Character Production; Associative Genome Mapping and mQTL Profiles; Large-scale Phenotyping Using Metabolomics; Conclusion and Outlook; References; Chapter 6 Physionomics; Introduction; Early Studies on Plant Physiology and the Discovery of Photosynthesis; Biochemical Approaches to Plant Physiology and the Discovery of Plant Hormones; Genetic Approaches to Plant Physiology and the Discovery of Hormone Signal Transduction Pathways.
- Alternative Genetic Models for Omics Approaches in Plant Physiology""Physionomics"" as an Integrator of Various Omics for Functional Studies and Plant Breeding; Acknowledgements; References; Chapter 7 Phenomics; Introduction; Examples of Large-scale Phenotyping; Important Aspects for Phenomics Implementation; Sampling versus Controlling Environmental Variation; Storage and Data Management; Statistical Analysis of Data; Main Breeding Applications; Germplasm Characterization; Genomic Selection (GS); QTL Identification; Final Considerations; References.