Combinatorial libraries : synthesis, screening, and application potential /

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Cortese, Riccardo
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Walter de Gruyter, 1996.
Temas:
Nucleotide sequence.
Amino acid sequence.
Genetic vectors.
Base Sequence
Amino Acid Sequence
Genetic Vectors
Séquence des acides aminés.
Vecteurs de clonage.
SCIENCE > Life Sciences > Biology.
Amino acid sequence
Genetic vectors
Nucleotide sequence
Séquence nucléotidique.
Séquençage des acides aminés.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Contents
  • A Synthetic Peptide Libraries
  • 1 Soluble Synthetic Combinatorial Libraries: The Use of Molecular Diversities for Drug Discovery
  • 1.1 Introduction
  • 1.2 Synthetic Combinatorial Libraries (SCLs)
  • 1.3 Synthetic Methods for the Generation of SCLs
  • 1.4 SCLs in Drug Discovery and Basic Research
  • 1.5 Conclusion
  • 2 Combinatorial Libraries of Synthetic Structures: Synthesis, Screening, and Structure Determination
  • 2.1 Introduction
  • 2.2 One-Bead One-Structure Concept
  • 2.3 Design and Synthesis of Non-Peptide Libraries
  • 2.4 Release Assay
  • 2.5 Stucture Determination2.6 Conclusion
  • 3 Peptide Libraries Bound to Continuous Cellulose Membranes: Tools to study Molecular Recognition
  • 3.1 Introduction
  • 3.2 Detection of antibody epitopes
  • 3.3 Mutational analyses of peptide epitopes
  • 3.4 Positional scanning combinatorial library
  • 3.5 Indentification of metal binding peptides
  • 3.6 Summary
  • B Nucleic Acids Libraries
  • 4 In Vitro Selection of Nucleic Acid Sequences that Bind Small Molecules
  • 4.1 Introduction
  • 4.2 Natural RNA Receptors
  • 4.3 In Vitro Selection
  • 4.4 Amino Acid Aptamers4.5 Cofactors
  • 4.6 DNA Aptamers
  • 4.7 The Complexity of Complexity
  • 4.8 Aptamer Structures
  • 4.9 Transition State Stabilization and Tight Binding
  • 4.10 Conclusions
  • 5 Discovery and Characterization of a Thrombin Aptamer Selected from a Combinatorial ssDNA Library
  • 5.1 Introduction
  • 5.2 Discovery and Initial Characterization
  • 5.3 Structure
  • 5.4 Binding Site on Thrombin
  • 5.5 Determination of Kd and Ki
  • 5.6 In vitro Activity
  • 5.7 In Vitro Activity
  • 5.8 Conclusions
  • C Phage Display of Peptide Libraries
  • 6 Structural and Functional Constraints in the Display of Peptides on Filamentous Phage Capsids6.1 Introduction
  • 6.2 The Phage Life Cycle
  • 6.3 A Low Resolution Model
  • 6.4 Extending the Amino-Terminus of pVIII
  • 6.5 Modifying the Surface of Filamentous Phage by Amino Acid Substitution
  • 6.6 Can the Remaining Minor Proteins Support Modification?
  • 7 Conformationally Defined Peptide Libraries on Phage: Selectable Templates for the Design of Pharmacological Agents
  • 7.1 Introduction
  • 7.2 From Peptides to Peptidomimetics
  • 7.3 Building Constraints in Phage-Displayed Polypeptides7.4 The Minibody: An Engineered ß-Pleated Scaffold for the Display of Reverse-Turn Motifs
  • 7.5 The Zinc Finger: A Small Domain for the Display of Structurally Homogeneous α-Helical Motifs
  • 7.6 Future Developments: Progressing Toward Non-Peptide Pharmaceuticals
  • 8 Discovery of Disease-Specific Mimotopes by Screening Phage Libraries with Human Serum Samples
  • 8.1 Introduction
  • 8.2 Using Polyclonal Antibodies as a Ligate for the Selection of RPL

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