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Applications of Plant Tissue Culture /
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
[Place of publication not identified] :
New Age International,
2011.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover ; Preface ; Acknowledgements ; Contents ; Chapter 1 Introduction ; 1.1 Definition to Tissue Culture ; 1.2 Shoot-Tip and Meristem-Tip Culture ; 1.3 Important Contributors to Plant Tissue Culture ; Chapter 2 Laboratory Organization ; 2.1 Tissue Culture Laboratory Setup ; 2.2 Essential Equipments ; 2.3 Control of Growing Conditions ; 2.4 Green House Facility ; Chapter 3 Methods of Sterilization ; 3.1 Contaminants ; 3.2 Laminar Air Flow (LAF) ; 3.3 Sterilization of Glass Ware ; 3.4 Wet Heat Sterilization: Autoclave ; 3.5 Sterilization of Media and Plant Tissues.
- 3.6 Preparation of Stock Plant and Stock Solution Chapter 4 Methods of Media Preparation ; 4.1 Medium Stock Solutions ; 4.2 Preparation of Molar Solution ; Chapter 5 Ingredients of Culture Media and Their Functions ; 5.1 Media Components ; 5.2 Media Formulations ; 5.3 Plant Growth Regulators ; 5.4 Light and the Growth Response ; Chapter 6 Plant Hormones ; 6.1 Plant Hormones ; 6.2 Auxins ; 6.3 Cytokinins ; 6.4 Gibberellins ; 6.5 Abscisic Acid ; 6.6 Ethylene ; 6.7 Other Potential PGRS ; Chapter 7 Micro Propagation ; 7.1 Shoot Meristems ; 7.2 Advantages of Shoot Culture.
- 7.3 Disadvantages of Shoot Culture Chapter 8 Somatic Embryogenesis ; 8.1 Totipotency of the Plant Cell ; 8.2 Uses of Somatic Embryogenesis ; Chapter 9 Synthetic Seeds ; 9.1 Introduction ; 9.2 General Method and Uses ; 9.3 Preparation of Synthetic Seeds ; Chapter 10 Bioreactors as a Low Cost Option for Tissue Culture ; 10.1 Bioreactor Vessels ; 10.2 Types of Bioreactors ; 10.3 Advantages and Disadvantages ; 10.4 Construction of a Simple Bioreactor ; Chapter 11 Disease Detection and Elimination, Quality Assurance ; 11.1 Various Contaminations and Infections ; 11.2 Elisa Test.
- 11.3 Polymerase Chain Reaction 11.4 Nucleic Acid Hybridization Test ; 11.5 Elimination of Viruses ; 11.6 Quality Assurance ; 11.7 DNA Based Molecular Markers ; Chapter 12 Applications of Biotechnology in Horticulture ; 12.1 Tissue Culture ; 12.2 Genetic Engineering of Plants ; 12.3 Engineering Pathogen Resistance ; 12.4 Fruit Quality ; 12.5 Male Sterility and Fertility Restoration ; 12.6 Molecular Diagnostics ; 12.7 Molecular Markers ; 12.8 Development of Beneficial Microbes ; Chapter 13 Genetic Transformation of Plants By Agrobacterium Tumefaciens.
- 13.1 The Mechanism of T-DNA Transfer From Agrobacterium to the Plant Cell 13.2 Agrobacterium As A Biotechnological Tool ; 13.3 Monocots Transformation ; 13.4 Culture Media ; Chapter 14 Polythylene Glycol (PEG) Mediated Direct Gene Transfer to Plants ; 14.1 Procedure and Solutions ; 14.2 Isolation of Mesophyll Protoplasts From Tobacco Shoots Cultures ; 14.3 Direct Gene Transfer to Protoplasts Using Polyethylene Glycol ; 14.4 Culture of Protoplasts ; 14.5 Isolation of Genomic DNA From Plant Tissue ; Chapter 15 Status of Cryopreservation Technologies in Plants (Crops and Forest Trees).