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Practical Flow Cytometry in Haematology Diagnosis.

This book acts as a clinical manual for the diagnostician who cannot turn to reference books when the morphology or immunophenotype are atypical. This volume presents a logical practical approach to the diagnosis of blood disorders, both neoplastic and reactive, and other diagnostic applications of...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Leach, Mike
Otros Autores: Drummond, Mark, Doig, Allyson
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Chicester : Wiley, 2013.
Temas:
Acceso en línea:Texto completo

MARC

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050 4 |a RC280.H47 .L384 2013 
060 4 |a WH 120 
082 0 4 |a 616.15075 
049 |a UAMI 
100 1 |a Leach, Mike. 
245 1 0 |a Practical Flow Cytometry in Haematology Diagnosis. 
260 |a Chicester :  |b Wiley,  |c 2013. 
300 |a 1 online resource (258 pages) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
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505 0 |a Practical Flow Cytometry in Haematology Diagnosis; Copyright; Contents; Preface; Acknowledgements; Chapter 1 Introduction; References; Chapter 2 Principles of Flow Cytometry; Introduction; Sample preparation; Direct staining method for surface immunophenotyping of peripheral blood or bone marrow (lyse -- no wash method); Surface immunoglobulin staining; Intracellular Staining; The flow cytometer; The fluidic system; The optical system; Light scatter; Fluorescence; Fluorescence intensity; Spectral overlap; The electronic system; Threshold; Data display; Histogram; Dot plot. 
505 8 |a Instrument set-up and quality controlAcknowledgements; References; Chapter 3 Limitations; Introduction; Clinical context issues; Sampling issues; Blood; Bone marrow aspirates; Effusions; Cerebrospinal fluid; Regenerating bone marrows; Technical issues; Operator training; Antibody and fluorochrome selection; Background fluorescence; Interpretation issues; Morphological correlation; Clinical context; Combined report; Conclusion; References; Chapter 4 Normal Blood and Bone Marrow Populations; Normal stem and precursor cell populations; Haematopoietic progenitor cells; Myeloid maturation. 
505 8 |a Erythroid maturationMegakaryocytic maturation; Lymphoid maturation; Haematogones; References; Chapter 5 Acute Leukaemia; Introduction; Acute leukaemia: definition and classification; Clinical presentation and sampling for flow cytometry; Identification of neoplastic precursor cells; The blast population; Blast populations are heterogeneous; Correlating the immunophenotype with genetic analysis; Blast cell characteristics; Patterns of precursor antigen expression in acute leukaemia; Enumeration of blast cells; Lineage assignment of acute leukaemia; Lineage assignment in acute myeloid leukaemia. 
505 8 |a Lineage assignment in acute lymphoblastic leukaemiaAcute leukaemia of ambiguous lineage; Acute myeloid leukaemia; Aberrant and asynchronous antigen expression in acute myeloid leukaemia; Antigenic patterns of particular significance in acute myeloid leukaemia; CD34 -negative HLA-DR-negative acute myeloid leukaemia; Identifying particular acute myeloid leukaemia subtypes by flow; Precursor lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma); B-cell acute lymphoblastic leukaemia/lymphoma; Aberrant antigen expression in B-acute lymphoblastic leukaemia. 
505 8 |a Identifying B-acute lymphoblastic leukaemia subtypes by flowT-cell acute lymphoblastic leukaemia/lymphoma; Aberrant antigen expression in T-acute lymphoblastic leukaemia; Examination of cerebro-spinal fluid and serous effusions; Central Nervous System Disease in Acute Leukaemia; Detection of acute leukaemia cells in serous effusions; Identification of genetic abnormalities by flow; Conclusion; References; Chapter 6 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma; Normal peripheral blood lymphoid populations; Identification of clonal lymphoid populations. 
500 |a Identification of clonal B-cell disorders. 
520 |a This book acts as a clinical manual for the diagnostician who cannot turn to reference books when the morphology or immunophenotype are atypical. This volume presents a logical practical approach to the diagnosis of blood disorders, both neoplastic and reactive, and other diagnostic applications of flow cytometry in non-neoplastic haematology diagnosis. Illustrations are provided throughout with worked examples. 
588 0 |a Print version record. 
504 |a Includes bibliographical references and index. 
590 |a ProQuest Ebook Central  |b Ebook Central Academic Complete 
650 0 |a Blood  |x Diseases  |x Diagnosis. 
650 0 |a Flow cytometry  |x Methodology. 
650 0 |a Hematology  |x Methodology. 
650 0 |a Blood  |x Tumors  |x Diagnosis. 
650 1 2 |a Hematologic Diseases  |x diagnosis 
650 1 2 |a Hematologic Neoplasms  |x diagnosis 
650 2 2 |a Flow Cytometry  |x methods 
650 2 2 |a Hematology  |x methods 
650 6 |a Sang  |x Maladies  |x Diagnostic. 
650 6 |a Cytométrie de flux  |x Méthodologie. 
650 6 |a Hématologie  |x Méthodologie. 
650 7 |a Blood  |x Diseases  |x Diagnosis  |2 fast 
650 7 |a Flow cytometry  |x Methodology  |2 fast 
700 1 |a Drummond, Mark. 
700 1 |a Doig, Allyson. 
776 0 8 |i Print version:  |a Leach, Mike.  |t Practical Flow Cytometry in Haematology Diagnosis.  |d Chicester : Wiley, ©2013  |z 9780470671207 
856 4 0 |u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=1120962  |z Texto completo 
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