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|a 616.15075
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|a UAMI
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|a Leach, Mike.
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|a Practical Flow Cytometry in Haematology Diagnosis.
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|a Chicester :
|b Wiley,
|c 2013.
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300 |
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|a 1 online resource (258 pages)
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a Practical Flow Cytometry in Haematology Diagnosis; Copyright; Contents; Preface; Acknowledgements; Chapter 1 Introduction; References; Chapter 2 Principles of Flow Cytometry; Introduction; Sample preparation; Direct staining method for surface immunophenotyping of peripheral blood or bone marrow (lyse -- no wash method); Surface immunoglobulin staining; Intracellular Staining; The flow cytometer; The fluidic system; The optical system; Light scatter; Fluorescence; Fluorescence intensity; Spectral overlap; The electronic system; Threshold; Data display; Histogram; Dot plot.
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|a Instrument set-up and quality controlAcknowledgements; References; Chapter 3 Limitations; Introduction; Clinical context issues; Sampling issues; Blood; Bone marrow aspirates; Effusions; Cerebrospinal fluid; Regenerating bone marrows; Technical issues; Operator training; Antibody and fluorochrome selection; Background fluorescence; Interpretation issues; Morphological correlation; Clinical context; Combined report; Conclusion; References; Chapter 4 Normal Blood and Bone Marrow Populations; Normal stem and precursor cell populations; Haematopoietic progenitor cells; Myeloid maturation.
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|a Erythroid maturationMegakaryocytic maturation; Lymphoid maturation; Haematogones; References; Chapter 5 Acute Leukaemia; Introduction; Acute leukaemia: definition and classification; Clinical presentation and sampling for flow cytometry; Identification of neoplastic precursor cells; The blast population; Blast populations are heterogeneous; Correlating the immunophenotype with genetic analysis; Blast cell characteristics; Patterns of precursor antigen expression in acute leukaemia; Enumeration of blast cells; Lineage assignment of acute leukaemia; Lineage assignment in acute myeloid leukaemia.
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|a Lineage assignment in acute lymphoblastic leukaemiaAcute leukaemia of ambiguous lineage; Acute myeloid leukaemia; Aberrant and asynchronous antigen expression in acute myeloid leukaemia; Antigenic patterns of particular significance in acute myeloid leukaemia; CD34 -negative HLA-DR-negative acute myeloid leukaemia; Identifying particular acute myeloid leukaemia subtypes by flow; Precursor lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma); B-cell acute lymphoblastic leukaemia/lymphoma; Aberrant antigen expression in B-acute lymphoblastic leukaemia.
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|a Identifying B-acute lymphoblastic leukaemia subtypes by flowT-cell acute lymphoblastic leukaemia/lymphoma; Aberrant antigen expression in T-acute lymphoblastic leukaemia; Examination of cerebro-spinal fluid and serous effusions; Central Nervous System Disease in Acute Leukaemia; Detection of acute leukaemia cells in serous effusions; Identification of genetic abnormalities by flow; Conclusion; References; Chapter 6 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma; Normal peripheral blood lymphoid populations; Identification of clonal lymphoid populations.
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500 |
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|a Identification of clonal B-cell disorders.
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|a This book acts as a clinical manual for the diagnostician who cannot turn to reference books when the morphology or immunophenotype are atypical. This volume presents a logical practical approach to the diagnosis of blood disorders, both neoplastic and reactive, and other diagnostic applications of flow cytometry in non-neoplastic haematology diagnosis. Illustrations are provided throughout with worked examples.
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|a Print version record.
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504 |
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|a Includes bibliographical references and index.
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590 |
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|a ProQuest Ebook Central
|b Ebook Central Academic Complete
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650 |
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0 |
|a Blood
|x Diseases
|x Diagnosis.
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650 |
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0 |
|a Flow cytometry
|x Methodology.
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650 |
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0 |
|a Hematology
|x Methodology.
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650 |
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0 |
|a Blood
|x Tumors
|x Diagnosis.
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650 |
1 |
2 |
|a Hematologic Diseases
|x diagnosis
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650 |
1 |
2 |
|a Hematologic Neoplasms
|x diagnosis
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650 |
2 |
2 |
|a Flow Cytometry
|x methods
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650 |
2 |
2 |
|a Hematology
|x methods
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650 |
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6 |
|a Sang
|x Maladies
|x Diagnostic.
|
650 |
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6 |
|a Cytométrie de flux
|x Méthodologie.
|
650 |
|
6 |
|a Hématologie
|x Méthodologie.
|
650 |
|
7 |
|a Blood
|x Diseases
|x Diagnosis
|2 fast
|
650 |
|
7 |
|a Flow cytometry
|x Methodology
|2 fast
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700 |
1 |
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|a Drummond, Mark.
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700 |
1 |
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|a Doig, Allyson.
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776 |
0 |
8 |
|i Print version:
|a Leach, Mike.
|t Practical Flow Cytometry in Haematology Diagnosis.
|d Chicester : Wiley, ©2013
|z 9780470671207
|
856 |
4 |
0 |
|u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=1120962
|z Texto completo
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