Forensic DNA Biology : a Laboratory Manual.

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DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DN...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Elkins, Kelly M.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Burlington : Elsevier Science, 2012.
Temas:
Forensic genetics > Technique.
DNA fingerprinting.
Polymerase chain reaction.
DNA Fingerprinting
Forensic Genetics > methods
Polymerase Chain Reaction
Sequence Analysis, DNA > methods
Génétique légale > Technique.
Empreintes génétiques.
Réaction en chaîne de la polymérase.
Polymerase chain reaction
DNA fingerprinting
Forensic genetics > Technique
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover; Forensic DNA Biology: A Laboratory Manual; Copyright; Contents; Acknowledgements; About the Author; Welcome; Forensic DNA Biology: An Introduction; BIOLOGY OVERVIEW; RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; POLYMERASE CHAIN REACTION; SHORT TANDEM REPEATS; SINGLE NUCLEOTIDE POLYMORPHISMS; MITOCHONDRIAL DNA; KNOWN VERSUS QUESTIONED SAMPLES; WHY STUDY FORENSIC DNA BIOLOGY?; Laboratory Safety; RULES FOR A SAFE LAB ENVIRONMENT; Reference; Avoiding Contamination Issues: Standard Laboratory Practices; Reference; Chapter 1
  • Pipetting; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND.
  • PROCEDUREQUESTIONS; GRAPHING THE DATA USING MICROSOFT EXCEL (2003); EQUATIONS; References; Chapter 2
  • Serology; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 3
  • Sampling Biological Evidence for DNA Extraction; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE: DNA COLLECTION AND PACKAGING; QUESTIONS; References; Chapter 4
  • DNA Extraction; OBJECTIVE; SAFETY; MATERIALS; RECIPES FOR BUFFER AND SOLUTION PREPARATION; BACKGROUND; PROCEDURE; QUESTION; References; Chapter 5
  • Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis.
  • OBJECTIVESAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; Reference; Chapter 6
  • Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 7
  • Determination of DNA Quantity by Fluorescence Spectroscopy; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 8
  • Real-Time Polymerase Chain Reaction (PCR) Quantitation of DNA; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References.
  • Chapter 9
  • Multiplex Polymerase Chain Reaction (PCR) Primer Design (in Silico)OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 10
  • Testing Designed Polymerase Chain Reaction (PCR) Primers in Multiplex Reactions; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 11
  • Multiplex Polymerase Chain Reaction (PCR) Amplification of Short Tandem Repeat (STR) Loci Using a Commercial Kit; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References.
  • Chapter 12
  • Capillary Electrophoresis of Short Tandem Repeat (STR) Polymerase Chain Reaction (PCR) Products from a Commercial Multiplex KitOBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTION; References; Chapter 13
  • Computing Random Match Probability from DNA Profile Data Using Population Databases; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References; Chapter 14
  • Mitochondrial Deoxyribonucleic Acid (mtDNA) Single Nucleotide Polymorphism (SNP) Detection; OBJECTIVE; SAFETY; MATERIALS; BACKGROUND; PROCEDURE; QUESTIONS; References.
  • Chapter 15
  • Analysis of Deoxyribonucleic Acid (DNA) Sequence Data Using BioEdit.

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