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Methods in Bioengineering.
Filling a critical gap in the current literature, this new resource presents practical, step-by-step methods to help you synthesize, characterize, biofunctionalize and apply the nanomaterial that is most suitable for handling a given nanoscale bioengineering problem. Written and presented by leading...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Norwood :
Artech House,
2009.
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| Colección: | Methods in bioengineering.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Methods in Bioengineering: Nanoscale Bioengineering and Nanomedicine; Contents; Preface; Chapter 1 Preparation and Characterization of Carbon Nanotube-Protein Conjugates; 1.1 Introduction; 1.2 Materials; 1.3 Methods; 1.3.1 Physical Adsorption of Proteins on Carbon Nanotubes; 1.3.2 Protein Assisted Solubilization of Carbon Nanotubes; 1.3.3 Covalent Attachment of Proteins onto Carbon Nanotubes; 1.4 Data Acquisition, Anticipated Results, and Interpretation of Data; 1.4.1 Characterization of Proteins Physically Adsorbed onto Carbon Nanotubes.
- 1.4.2 Characterization of Protein-Solubilized Carbon Nanotubes1.4.3 Characterization of Covalently Attached Carbon Nanotube-Protein Conjugates; 1.5 Discussion and Commentary; 1.6 Applications Notes; 1.7 Summary Points; Acknowledgments; References; Chapter 2 Peptide-Nanoparticle Assemblies; 2.1 Introduction; 2.2 Materials; 2.3 Methods; 2.3.1 Coil-Coil Peptide Mediated NP Assembly; 2.3.2 Synthesis of Hybrid Structures Using Multifunctional Peptides; 2.4 Assembly Mediated by Metal Ion-Peptide Recognition; 2.5 Peptides as Antibody Epitopes for Nanoparticle Assembly.
- 2.6 DATA Acquisition, Anticipated Results, and Interpretation2.7 Discussion and Commentary; 2.8 Application Notes; 2.9 Summary Points; Acknowledgments; References; Chapter 3 Nanoparticle-Enzyme Hybrids as Bioactive Materials; 3.1 Introduction; 3.2 Materials; 3.3 Methods; 3.3.1 Enzyme-Attached Polystyrene Nanoparticles; 3.3.2 Polyacrylamide Hydrogel Nanoparticles for Entrapment of Enzymes; 3.3.3 Magnetic Nanoparticles with Porous Silica Coating for Enzyme Attachment; 3.3.4 Enzyme Loading and Activity Assay; 3.4 Results; 3.4.1 Polystyrene-Enzyme Hybrid Nanoparticles.
- 3.4.2 Polyacrylamide Hydrogel Nanoparticles with Entrapped Enzymes3.4.3 Magnetic Nanoparticles for Enzyme Attachment; 3.5 Discussion and Commentary; 3.6 Troubleshooting; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Chapter 4 Self-Assembled QD-Protein Bioconjugates and Their Use in Fluorescence Resonance Energy Transfer; 4.1 Introduction; 4.2 Materials; 4.2.1 Reagents; 4.2.2 Equipment; 4.3 Methods; 4.3.1 Quantum Dot Synthesis; 4.3.2 Surface Ligand Exchange; 4.3.3 Biomolecule Conjugation; 4.3.4 Fluorescence Measurements; 4.4 Data Analysis and Interpretation.
- 4.4.1 Calculating Donor-Acceptor Distances4.4.2 Calculating Reaction Rates of Surface-Bound Substrates; 4.5 Summary Points; 4.6 Conclusions; References; Annotated References; Chapter 5 Tracking Single Biomolecules in Live Cells Using Quantum Dot Nanoparticles; 5.1 Introduction; 5.2 Materials; 5.2.1 Reagents; 5.2.2 Imaging Equipment; 5.3 Methods; 5.3.1 Forming QD Bioconjugates; 5.3.2 Treating Cells with QD Bioconjugates; 5.4 Data Acquisition, Anticipated Results, and Interpretation; 5.4.1 Imaging QD-Bound Complexes in Cells; 5.4.2 Analysis of the Real-Time QD Dynamics.