Cargando…
Methods in Bioengineering.
Filling a critical gap in the current literature, this new resource presents practical, step-by-step methods to help you synthesize, characterize, biofunctionalize and apply the nanomaterial that is most suitable for handling a given nanoscale bioengineering problem. Written and presented by leading...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Norwood :
Artech House,
2009.
|
| Colección: | Methods in bioengineering.
|
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
| LEADER | 00000cam a2200000Mu 4500 | ||
|---|---|---|---|
| 001 | EBOOKCENTRAL_ocn796382982 | ||
| 003 | OCoLC | ||
| 005 | 20240329122006.0 | ||
| 006 | m o d | ||
| 007 | cr unu|||||||| | ||
| 008 | 121121s2009 xx o 000 0 eng d | ||
| 040 | |a EBLCP |b eng |e pn |c EBLCP |d OCLCO |d OCLCQ |d ZCU |d MERUC |d ICG |d OCLCO |d OCLCF |d OCLCQ |d OCLCO |d OCLCQ |d DKC |d AU@ |d OCLCQ |d RDF |d OCLCO |d OCLCQ |d OCLCO |d OCLCL | ||
| 020 | |a 9781596934115 | ||
| 020 | |a 1596934115 | ||
| 029 | 1 | |a DEBBG |b BV044166457 | |
| 035 | |a (OCoLC)796382982 | ||
| 050 | 4 | |a R857 .N34 M48 2009 | |
| 060 | 4 | |a 2009 L-168 | |
| 060 | 4 | |a QT 36 |b M592 2009 | |
| 082 | 0 | 4 | |a 610.28 |a 610.28072 |
| 049 | |a UAMI | ||
| 100 | 1 | |a Rege, Kaushal. | |
| 245 | 1 | 0 | |a Methods in Bioengineering. |
| 260 | |a Norwood : |b Artech House, |c 2009. | ||
| 300 | |a 1 online resource (364 pages) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in Bioengineering | |
| 505 | 0 | |a Methods in Bioengineering: Nanoscale Bioengineering and Nanomedicine; Contents; Preface; Chapter 1 Preparation and Characterization of Carbon Nanotube-Protein Conjugates; 1.1 Introduction; 1.2 Materials; 1.3 Methods; 1.3.1 Physical Adsorption of Proteins on Carbon Nanotubes; 1.3.2 Protein Assisted Solubilization of Carbon Nanotubes; 1.3.3 Covalent Attachment of Proteins onto Carbon Nanotubes; 1.4 Data Acquisition, Anticipated Results, and Interpretation of Data; 1.4.1 Characterization of Proteins Physically Adsorbed onto Carbon Nanotubes. | |
| 505 | 8 | |a 1.4.2 Characterization of Protein-Solubilized Carbon Nanotubes1.4.3 Characterization of Covalently Attached Carbon Nanotube-Protein Conjugates; 1.5 Discussion and Commentary; 1.6 Applications Notes; 1.7 Summary Points; Acknowledgments; References; Chapter 2 Peptide-Nanoparticle Assemblies; 2.1 Introduction; 2.2 Materials; 2.3 Methods; 2.3.1 Coil-Coil Peptide Mediated NP Assembly; 2.3.2 Synthesis of Hybrid Structures Using Multifunctional Peptides; 2.4 Assembly Mediated by Metal Ion-Peptide Recognition; 2.5 Peptides as Antibody Epitopes for Nanoparticle Assembly. | |
| 505 | 8 | |a 2.6 DATA Acquisition, Anticipated Results, and Interpretation2.7 Discussion and Commentary; 2.8 Application Notes; 2.9 Summary Points; Acknowledgments; References; Chapter 3 Nanoparticle-Enzyme Hybrids as Bioactive Materials; 3.1 Introduction; 3.2 Materials; 3.3 Methods; 3.3.1 Enzyme-Attached Polystyrene Nanoparticles; 3.3.2 Polyacrylamide Hydrogel Nanoparticles for Entrapment of Enzymes; 3.3.3 Magnetic Nanoparticles with Porous Silica Coating for Enzyme Attachment; 3.3.4 Enzyme Loading and Activity Assay; 3.4 Results; 3.4.1 Polystyrene-Enzyme Hybrid Nanoparticles. | |
| 505 | 8 | |a 3.4.2 Polyacrylamide Hydrogel Nanoparticles with Entrapped Enzymes3.4.3 Magnetic Nanoparticles for Enzyme Attachment; 3.5 Discussion and Commentary; 3.6 Troubleshooting; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Chapter 4 Self-Assembled QD-Protein Bioconjugates and Their Use in Fluorescence Resonance Energy Transfer; 4.1 Introduction; 4.2 Materials; 4.2.1 Reagents; 4.2.2 Equipment; 4.3 Methods; 4.3.1 Quantum Dot Synthesis; 4.3.2 Surface Ligand Exchange; 4.3.3 Biomolecule Conjugation; 4.3.4 Fluorescence Measurements; 4.4 Data Analysis and Interpretation. | |
| 505 | 8 | |a 4.4.1 Calculating Donor-Acceptor Distances4.4.2 Calculating Reaction Rates of Surface-Bound Substrates; 4.5 Summary Points; 4.6 Conclusions; References; Annotated References; Chapter 5 Tracking Single Biomolecules in Live Cells Using Quantum Dot Nanoparticles; 5.1 Introduction; 5.2 Materials; 5.2.1 Reagents; 5.2.2 Imaging Equipment; 5.3 Methods; 5.3.1 Forming QD Bioconjugates; 5.3.2 Treating Cells with QD Bioconjugates; 5.4 Data Acquisition, Anticipated Results, and Interpretation; 5.4.1 Imaging QD-Bound Complexes in Cells; 5.4.2 Analysis of the Real-Time QD Dynamics. | |
| 520 | |a Filling a critical gap in the current literature, this new resource presents practical, step-by-step methods to help you synthesize, characterize, biofunctionalize and apply the nanomaterial that is most suitable for handling a given nanoscale bioengineering problem. Written and presented by leading scientists and engineers in their respective fields, the authors offer a clear and detailed understanding of how to carry out nanoparticle functionalization with biomolecules (including enzymes), nanoparticle analysis and characterization, in vitro evaluation of nanoparticles using different cell l. | ||
| 588 | 0 | |a Print version record. | |
| 590 | |a ProQuest Ebook Central |b Ebook Central Academic Complete | ||
| 650 | 0 | |a Biomedical engineering. | |
| 650 | 0 | |a Nanoelectronics. | |
| 650 | 2 | |a Biomedical Technology | |
| 650 | 2 | |a Biomedical Engineering | |
| 650 | 6 | |a Génie biomédical. | |
| 650 | 6 | |a Nanoélectronique. | |
| 650 | 7 | |a biomedical engineering. |2 aat | |
| 650 | 7 | |a Biomedical engineering |2 fast | |
| 650 | 7 | |a Nanoelectronics |2 fast | |
| 700 | 1 | |a Medintz, Igor L. | |
| 758 | |i has work: |a Methods in bioengineering (Text) |1 https://id.oclc.org/worldcat/entity/E39PCFKtYrt7fYH4VBcBKFftjy |4 https://id.oclc.org/worldcat/ontology/hasWork | ||
| 776 | 0 | 8 | |i Print version: |a Rege, Kaushal. |t Methods in Bioengineering: Nanoscale Bioengineering and Nanomedicine. |d Norwood : Artech House, ©2009 |z 9781596934108 |
| 830 | 0 | |a Methods in bioengineering. | |
| 856 | 4 | 0 | |u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=946539 |z Texto completo |
| 938 | |a ProQuest Ebook Central |b EBLB |n EBL946539 | ||
| 994 | |a 92 |b IZTAP | ||