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Methods in Bioengineering.
Offering a practical look into the field, this volume presents the science behind microscale device design and the engineering of its fabrication. Supported with dozens of full-color illustrations, this book offers you clear, step-by-step methods for the cell capture from whole blood, high-throughpu...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Norwood :
Artech House,
2009.
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| Colección: | Methods in bioengineering.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Methods in Bioengineering: Microdevices in Biology and Medicine; Contents; Preface; Chapter 1 Immunoaffinity Capture of Cells from Whole Blood; 1.1 Introduction; 1.2 Experimental Design; 1.3 Materials; 1.4 Methods; 1.4.1 Device fabrication; 1.4.2 Fluidic port punching; 1.4.3 Surface modification; 1.4.4 Cell capture; 1.4.5 Injecting blood into cassette; 1.4.6 Washing noncaptured cells with PBS; 1.4.7 Postcapture processing; 1.4.8 Immunofluorescence staining; 1.4.9 Giemsa staining protocol; 1.4.10 Cell lysis for genomic applications; 1.5 Data Acquisition, Anticipated Results, and Interpretation.
- 1.6 Discussion and Commentary1.7 Application Notes; 1.8 Summary Points; Acknowledgments; References; Chapter 2 Dynamic Gene-Expression Analysis in a Microfluidic Living Cell Array (mLCA); 2.1 Introduction; 2.2 Materials; 2.2.1 Reagents; 2.2.2 Fabrication facilities; 2.2.3 Imaging equipment; 2.2.4 Perfusion components; 2.3 Methods; 2.3.1 GFP reporter cell line construction; 2.3.2 Microfluidic cell array fabrication; 2.3.3 Microfluidic array pretreatment and seeding; 2.3.4 Stimulation and reporter imaging; 2.4 Data Acquisition, Anticipated Results, and Interpretation; 2.5 Discussion.
- 2.6 Application Notes2.7 Summary Points; Acknowledgments; References; Chapter 3 Micromechanical Control of Cell-Cell Interactions; 3.1 Introduction; 3.1.1 Cell-cell interactions; 3.1.2 Conventional cocultivation models; 3.1.3 Micromechanical reconfigurable culture; 3.1.4 Application examples; 3.2 Experimental Design; 3.2.1 Experimental variables; 3.2.2 Readout; 3.3 Materials; 3.3.1 Reagents/supplies; 3.3.2 Facilities/equipment; 3.4 Methods; 3.4.1 Device handling and actuation; 3.4.2 Preparing devices for cell culture; 3.4.3 Cell seeding; 3.4.4 Assay preparation; 3.5 Discussion.
- 3.6 Summary PointsAcknowledgments; References; Related sources; Chapter 4 Mechanotransduction and the Study of Cellular Forces; 4.1 Introduction; 4.1.1 Cellular forces: Functions and underlying mechanisms; 4.1.2 Techniques for studying traction forces; 4.2 Materials; 4.2.1 Reagents and supplies; 4.2.2 Facilities, equipment, and software; 4.3 Methods; 4.3.1 Microfabrication of micropost arrays; 4.3.2 Analysis of traction forces with micropost arrays; 4.4 Discussion; 4.4.1 Applications and enhancements of the micropost arrays; 4.4.2 Potential pitfalls of micropost arrays.
- 4.4.3 Biological insights from using micropost arrays4.4.4 Future innovations for studying cellular forces; 4.5 Summary Points; References; Chapter 5 A Microfluidic Tool for Immobilizing C. elegans; 5.1 Introduction; 5.2 Materials; 5.3 Methods; 5.3.1 Overview and timeline; 5.3.2 Designing the device and ordering the photomask; 5.3.3 Fabricating the master for the device; 5.3.4 Replica-molding the master in PDMS; 5.3.5 Preparing C. elegans for loading; 5.3.6 Assembling the microfluidic device; 5.3.7 Preparing the device for loading; 5.3.8 Loading worms into the device.