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Methods in Bioengineering.
This practical book is part of the new Artech House Methods in Bioengineering series? volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. Written and edited by recognized experts in the field, each book provides research engineers,...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Norwood :
Artech House,
2010.
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| Colección: | Methods in bioengineering.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Methods in Bioengineering: 3D Tissue Engineering; Contents; Preface; Chapter 1 Chemical Modification of Porous Scaffolds Using Plasma Polymers; 1.1 Introduction; 1.2 Experimental Design; 1.3 Materials; 1.4 Methods; 1.4.1 Scaffold preparation; 1.4.2 Deposition of plasma polymers; 1.4.3 Surface analysis; 1.4.4 Cell culture on scaffolds; 1.4.5 Alamar Blue assay; 1.4.6 Cell viability assay on scaffolds; 1.4.7 Scanning electron microscopy; 1.4.8 Microcomputed tomography of scaffolds; 1.5 Data Acquisition, Anticipated Results, and Interpretation; 1.5.1 Surface analysis.
- 1.5.2 Investigation of cell culture on modified scaffolds1.6 Discussion and Commentary; 1.7 Application Notes; 1.8 Summary Points; Acknowledgments; References; Chapter 2 Three-Dimensional Cultures in Soft Self-Assembling Nanofibers; 2.1 Introduction; 2.2 Experimental Design; 2.3 Materials; 2.3.1 Reagents; 2.3.2 Equipment; 2.4 Methods; 2.4.1 Self-assembling peptide preparation; 2.4.2 Cell encapsulation into the self-assembling peptide; 2.4.3 Sandwich method; 2.4.4 Cell isolation and culture of isolated cells; 2.4.5 Cryosections of the 3D cultures.
- 2.4.6 Cell proliferation study using 5-bromodeoxyuridine (BrdU) uptake analysis2.4.7 Cell viability; 2.4.8 Protein analysis; 2.4.9 Sample staining; 2.4.10 sGAG quantification; 2.4.11 Lysis of 3D cultures for RNA extraction; 2.5 Data Acquisition, Anticipated Results, and Interpretation; 2.6 Discussion and Commentary; 2.7 Troubleshooting; 2.8 Application Notes; 2.9 Summary Points; Acknowledgments; References; Chapter 3 3D Fibrin Matrices as Scaffold for Depot and Release of Bioactive Molecules; 3.1 Introduction; 3.2 Experimental Design; 3.3 Materials; 3.3.1 Chemicals.
- 3.3.2 Equipment/Infrastructure3.4 Methods; 3.4.1 Preparation of 3D fibrin matrices; 3.4.2 Introduction of bioactive molecules; 3.4.3 Cell Culture; 3.4.4 Data acquisition, important controls, and staining procedures; 3.5 Data Analysis, Anticipated Results, and Interpretation; 3.6 Discussion and Commentary; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Selected Bibliography; Chapter 4 Designer Self-Assembling Peptide Scaffolds for 3D Tissue Cell Cultures; 4.1 Introduction; 4.1.1 Discovery and development of self-assembling peptide scaffolds.
- 4.1.2 The nanofiber structure of the peptide scaffold4.1.3 A generic biological scaffold; 4.1.4 Peptide scaffold fosters chondrocyte extracellular matrix production; 4.1.5 Designer peptides appended with active motifs; 4.2 Materials; 4.3 Reagents; 4.4 Methods; 4.4.1 Peptide solution preparation; 4.4.2 Designer peptide synthesis and scaffold preparation; 4.4.3 Culture cells in plate inserts; 4.4.4 Cell culture system; 4.4.5 Neural cell culture and seeding; 4.4.6 Preparation of MC3T3-E1 cells; 4.4.7 Cell culture of human umbilical vein endothelial cells (HUVECs); 4.4.8 Cell proliferation assay.