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Molecular Analysis and Genome Discovery.
Molecular Analysis and Genome Discovery, Second Edition is a completely revised and updated new edition of this successful book. The text provides a comprehensive overview of recent developments in the fast moving field of molecular based diagnostics of disease markers. Key concepts and applications...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Hoboken :
John Wiley & Sons,
2011.
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| Edición: | 2nd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Title Page; Copyright; Preface; Contributors; Chapter 1: Overview of Genotyping; Introduction; Methods for interrogating SNPs; Commercial platforms for SNP genotyping; Practical recommendations; SNP databases; Methylation analysis; Copy number variation analysis; Second generation sequencing technologies; Conclusions; References; Chapter 2: DNA Chip Analysis in Genome Discovery; Introduction; Interrogating a genome; Cross-species hybridization; Comparative genomic hybridization and microarray-based genotyping; Barcodes, DNA microarrays and organism identification; Concluding remarks.
- Determining a temperature window for meltingDyes and platforms for melt profiling; Scanning PCR products for sequence variation; Genotyping with high-resolution melt profiling; Other applications of HRMP; Final notes; References; Chapter 6: Massively Parallel Sequencing; Sanger sequencing; Massively parallel sequencing; Commercially available massively parallel sequencers; Future technologies; Paired-end or mate-paired reads; Target-enrichment strategies for MPS; Applications of MPS; Summary; References; Chapter 7: Aptamers for Analysis: Nucleic Acids Ligands in the Post-Genomic Era.
- IntroductionSELEX; Aptamers in analysis; Imaging with aptamers; Conclusions, outlooks and perspectives; References; Chapter 8: Use of Nanotechnology for Enhancing of Cancer Biomarker Discovery and Analysis: A Molecular Approach; Introduction; Proteomics and nanotechnology; Nanoscale multicomponent separation; Nanoscale protein detection strategies; Surface-enhanced Raman scattering (SERS); References; Chapter 9: Chip-Based Proteomics; Introduction; Lab-on-a-chip; Arrays; Chip-based mass spectrometry; Surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) chip instruments.
- MicrofluidicsConclusion; References; Chapter 10: Antibody Microarrays in Proteome Profiling; Introduction; Technical aspects; Antibody array applications; Summary; Acknowledgements; References; Chapter 11: Biomarker Detection and Molecular Profiling by Multiplex Microbead Suspension Array Based Immunoproteomics; Introduction; Principles of microbead-based multiplexing; Experimental aspects of the multiplex microbead assay; Multiplex microbead assay design and comparison with other methods; Applications of the multiplex microbead assay system for biomedical research and clinical studies.