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Mass Spectrometry Handbook.
Due to its enormous sensitivity and ease of use, mass spectrometry has grown into the analytical tool of choice in most industries and areas of research. This unique reference provides an extensive library of methods used in mass spectrometry, covering applications of mass spectrometry in fields as...
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Hoboken :
John Wiley & Sons,
2012.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; WILEY SERIES ON PHARMACEUTICAL SCIENCE AND BIOTECHNOLOGY: PRACTICES, APPLICATIONS AND METHODS; Title page; Copyright page; FOREWORD; PREFACE; CONTRIBUTORS; SECTION I: BIOTECHNOLOGY/PROTEINS; 1 TARGETED PROTEOMICS USING IMMUNOAFFINITY PURIFICATION; 1.1 INTRODUCTION; 1.2 EXPERIMENTAL PROTOCOLS; 1.3 APPLICATIONS OF THE PROTOCOLS; 1.4 CONCLUSION; ACKNOWLEDGMENTS; 2 MASS SPECTROMETRY-BASED METHODS TO INVESTIGATE POSTTRANSLATIONAL PROTEIN MODIFICATIONS BY LIPID PEROXIDATION PRODUCTS; 2.1 PROCEDURE CONTROL.
- 2.2 IDENTIFICATION OF HNE-MODIFIED PEPTIDES IN BIOLOGICAL SAMPLES BY SOLID-PHASE ENRICHMENT AND NANO-LC-ESI-MS/MS2.3 REAGENTS AND STANDARDS; 2.4 COMMENTARY; ACKNOWLEDGMENTS; 3 IMAGING MASS SPECTROMETRY (IMS) FOR BIOLOGICAL APPLICATION; 3.1 INTRODUCTION; 3.2 APPLICATIONS OF MALDI-IMS; 3.3 EXPERIMENTAL PROCEDURES; 3.4 STATISTICAL PROCEDURES FOR IMS DATA ANALYSIS; 4 METHODOLOGIES FOR IDENTIFYING MICROORGANISMS AND VIRUSES BY MASS CATALOGING OF RNAs; 4.1 INTRODUCTION: THE IMPORTANCE OF MICROBIAL GENOTYPING; 4.2 INFORMATICS: ENABLING ASPECTS OF 16S RRNA AND DATABASE CONSTRUCTION.
- 4.3 EXPERIMENTAL METHODS: PCR, TRANSCRIPTION, AND ENZYMATIC FRAGMENTATION4.4 DATABASE COMPARISON AND RESULTS: TYPICAL RESULTS OF BACTERIAL AND VIRAL GENOTYPING; 4.5 DISCUSSION; ACKNOWLEDGMENTS; SECTION II: PHARMACEUTICAL; 5 PRECLINICAL PHARMACOKINETICS: INDUSTRIAL PERSPECTIVE; 5.1 INTRODUCTION; 5.2 A PHARMACOKINETICS PRIMER; 5.3 PARAMETERS THAT DEFINE PHARMACOKINETIC PROFILE; 5.4 MODELING TO PREDICT SINGLE- AND MULTIPLE-DOSE PHARMACOKINETIC PROFILES; 5.5 ROLE OF LC/MS/MS IN PHARMACOKINETIC ASSESSMENT IN DRUG DISCOVERY.
- 6 LC-MS IN DRUG METABOLISM AND PHARMACOKINETICS: A PHARMACEUTICAL INDUSTRY PERSPECTIVE6.1 INTRODUCTION; 6.2 INSTRUMENTATION UTILIZED FOR DMPK STUDIES; 6.3 LC-MS FOR IN VITRO ADME SCREENING; 6.4 LC-MS FOR IN VIVO BIOANALYSIS IN SUPPORT OF DMPK; 6.5 LC-MS IN DRUG METABOLITE PROFILING AND IDENTIFICATION; 6.6 SUMMARY AND PERSPECTIVE; ACKNOWLEDGMENTS; 7 QUANTITATIVE MASS SPECTROMETRY IN SUPPORT OF PHARMACOKINETIC STUDIES; 7.1 INTRODUCTION; 7.2 METHODOLOGY; 7.3 CURRENT PROTOCOL; 7.4 DEFINITIONS; 8 DETERMINATION OF PHARMACOKINETIC PARAMETERS BY HPLC-MS/MS AND UPLC-MS/MS; 8.1 INTRODUCTION.
- 8.2 LC-MS/MS ASSAYS FOR PK DETERMINATION IN DRUG DISCOVERY8.3 PK EVALUATION OF LEAD CANDIDATE AND METABOLITES IN PRECLINICAL DEVELOPMENT; 8.4 PK ASSAYS FOR DRUG CANDIDATE DURING CLINICAL DEVELOPMENT; 8.5 CONCLUSION; 9 METHODS FOR SCREENING ENANTIOSELECTIVE INTERACTIONS IN THE SOLUTION PHASE USING ESI-MS; 9.1 INTRODUCTION; 9.2 ENANTIOSELECTIVE DISCRIMINATION BY MS; 9.3 MEASURING ENANTIOSELECTIVE DISCRIMINATION IN SOLUTION WITH ESI-MS; 9.4 EMERGING METHODS AND OUTLOOK.