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NMR of Biomolecules.
NMR is one of the most powerful methods for imaging of biomolecules. This book is the ultimate NMR guide for researchers in the biomedical community and gives not only background and practical tips but also a forward looking view on the future of NMR in systems biology.
| Clasificación: | Libro Electrónico |
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| Autor principal: | |
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Hoboken :
John Wiley & Sons,
2012.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover; Related Titles; Title Page; Copyright; Preface; List of Contributors; List of Abbreviations; Part One: Introduction; Chapter 1: NMR and its Place in Mechanistic Systems Biology; Chapter 2: Structure of Biomolecules: Fundamentals; 2.1 Structural Features of Proteins; 2.2 Nucleic Acids; Chapter 3: What Can be Learned About the Structure and Dynamics of Biomolecules from NMR; 3.1 Proteins Studied by NMR; 3.2 Nucleic Acids Studied by NMR; Part Two: Role of NMR in the Study of the Structure and Dynamics of Biomolecules; Chapter 4: Determination of Protein Structure and Dynamics.
- 4.1 Determination of Protein Structures4.2 NMR Restraints; 4.3 Structure Calculations; 4.5 Protein Dynamics and NMR Observables; 4.6 Protocols; 4.7 Troubleshooting; Further Reading; Chapter 5: DNA; 5.1 NMR studies of DNA; 5.2 Assessment of the Folding Topology; 5.3 Resonance Assignment through Sequential and Interstrand Interactions; 5.4 Pseudorotation of Deoxyribofuranose Rings; 5.5 Backbone Conformation; 5.6 Natural Abundance Nucleobase Substitutions; 5.7 Natural Abundance Heteronuclear Experiments; 5.8 Site-Specific Low Isotopic Enrichment; 5.9 Translational Diffusion Coefficients.
- 5.10 Determination of Three-Dimensional Structure5.11 Search for Transient Structures; 5.12 Protocols; 5.13 Example Experiments and Troubleshooting; Acknowledgments; Further Reading; Chapter 6: RNA; 6.1 NMR Spectroscopy of RNA; 6.2 Preparation of RNA Samples for NMR; 6.3 Probing of the RNA Fold; 6.4 Assessment of the Spectral Resolution; 6.5 Strategy for the Resonance Assignment; 6.6 Collection of Structural Information; 6.7 Structural Calculation of RNA; 6.8 Assessment of Quality of NMR Structures; 6.9 Protocols; 6.10 Troubleshooting; Acknowledgments; Further Reading.
- Chapter 7: Intrinsically Disordered Proteins7.1 Intrinsically Disordered Proteins; 7.2 Importance of NMR to Study IDPs; 7.3 Structural and Dynamic Information on IDPs
- NMR Observables; 7.4 Protocols; 7.5 Troubleshooting; Further Reading; Chapter 8: Paramagnetic Molecules; 8.1 Paramagnetism-Assisted NMR; 8.2 Scalar and Dipolar Electron Spin-Nuclear Spin Interactions: Hyperfine Shift; 8.3 Scalar and Dipolar Electron Spin-Nuclear Spin Interactions: PRE; 8.4 Indirect Electron Spin-Nuclear Spin Effects: Paramagnetism-Induced RDCs; 8.5 Cross-Correlation Between Curie and Dipolar Relaxation.
- 8.6 "Good" Metal Ions and "Bad" Metal Ions8.7 Paramagnetism-Based Drug Discovery; 8.8 Protocols; 8.9 Troubleshooting; Further Reading; Part Three: Role of NMR in the Study of the Structure and Dynamicsof Biomolecular Interactions; Chapter 9: NMR Methodologies for the Analysis of Protein-Protein Interactions; 9.1 Introduction; 9.2 Dynamics and Ligand Binding; 9.3 General Strategy; 9.4 Overview of Methods; 9.5 Outlook; 9.6 Protocols for the Analysis of Protein Complexes; 9.7 Troubleshooting; Further Reading; Chapter 10: Metal-Mediated Interactions; 10.1 Theoretical Background.