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Alternative Pre-mRNA Splicing : Theory and Protocols /
This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing intwelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. Thes...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Weinheim :
Wiley-Blackwell,
©2012.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Matter
- Theory. Splicing in the RNA World / Emanuele Buratti, Maurizio Romano, Francisco E Baralle
- RNPs, Small RNAs, and miRNAs / Michaela Beitzinger, Gunter Meister
- RNA Elements Involved in Splicing / William F Mueller, Klemens J Hertel
- A Structural Biology Perspective of Proteins Involved in Splicing Regulation / Antoine Cľry, Fřďric H-T Allain
- The Spliceosome in Constitutive Splicing / Patrizia Fabrizio, Reinhard Luhrmann
- The Use of to Study the Mechanism of pre-mRNA Splicing / Brian C Rymond
- Challenges in Plant Alternative Splicing / Andrea Barta, Yamile Marquez, John W S Brown
- Alternative Splice Site Selection / Miguel B Coelho, Christopher W J Smith
- Integration of Splicing with Nuclear and Cellular Events / Aparna K Sapra, Karla Neugebauer
- Splicing and Disease / Emanuele Buratti, Francisco E Baralle
- From Bedside to Bench: How to Analyze a Splicing Mutation / Marco Baralle, Diana Baralle
- Basic Methods. Analysis of Common Splicing Problems / Stefan Stamm
- Ultracentrifugation in the Analysis and Purification of Spliceosomes Assembled / Klaus Hartmuth, Maria A van Santen, Reinhard Luhrmann
- Chemical Synthesis of RNA / Claudia H̲bartner
- RNA Interference (siRNA, shRNA) / Daphne S Cabianca, Davide Gabellini
- Expression and Purification of Splicing Proteins / James Sťvenin, Cyril F Bourgeois
- Detection of RNA-Protein Complexes by Electrophoretic Mobility Shift Assay / Manli Shen, Michael G Fried
- Functional Analysis of Large Exonic Sequences Through Iterative Selection / Ravindra N Singh, Natalia N Singh
- Identification of Splicing cis-Elements Through an Ultra-Refined Antisense Microwalk / Natalia N Singh, Joonbae Seo, Ravindra N Singh
- Genomic SELEX to Identify RNA Targets of Plant RNA-Binding Proteins / Olga Bannikova, Maria Kalyna, Andrea Barta
- Detection of Splicing Events. Quantification of Alternative Splice Variants / Miriam Llorian, Christopher W J Smith
- High-Throughput Analysis of Alternative Splicing by RT-PCR / Roscoe Klinck, Benoit Chabot, Sherif Abou Elela
- Monitoring Changes in Plant Alternative Splicing Events / Craig G Simpson, Naeem Hasan Syed, Sujatha Manthri, John D Fuller, Monika Maronova, Branislav Kusenda, Maria Kalyna, Andrea Barta, John WS Brown
- Array Analysis / Pierre de la Grange
- The CLIP Method to Study Protein-RNA Interactions in Intact Cells and Tissues / James Tollervey, Jernej Ule
- RNA₆Protein Crosslinking and Immunoprecipitation (CLIP) in / Branislav Kusenda, Andrea Barta
- Identification of Proteins Bound to RNA / Emanuele Buratti
- Single-Cell Detection of Splicing Events with Fluorescent Splicing Reporters / Hidehito Kuroyanagi, Akihide Takeuchi, Takayuki Nojima, Masatoshi Hagiwara
- Analysis of Splicing . The Preparation of HeLa Cell Nuclear Extracts / Klaus Hartmuth, Maria A van Santen, Tanja R̲sel, Berthold Kastner, Reinhard Luhrmann
- Splicing Assays / Akila Mayeda, Adrian R Krainer
- Assembly and Isolation of Spliceosomal Complexes / Klaus Hartmuth, Maria A van Santen, Peter Odenw̃lder, Reinhard Luhrmann
- Analysis of Site-Specific RNA-Protein Interactions / Nathalie Marmier-Gourrier, Audrey Vautrin, Christiane Branlant, Isabelle Behm-Ansmant
- Immunoprecipitation and Pull-Down of Nuclear Proteins / Natalya Benderska, Chiranthani Sumanasekera, Stefan Stamm
- Analysis of Protein ( -RNA) Complexes by (Quantitative) Mass Spectrometric Analysis / Carla Schmidt, Henning Urlaub
- Fast Cloning of Splicing Reporter Minigenes / Zhaiyi Zhang, Amit Khanna, Stefan Stamm
- Analysis of Splicing Assays / Isabel C L̤pez-Mej̕a, Jamal Tazi
- Coupled Promoter Splicing Systems / Manuel J Munoz, Manuel de la Mata, Alberto R Kornblihtt
- Stable Cell Lines with Splicing Reporters / Christian Kroun Damgaard, Soren Lykke-Andersen, J̜rgen Kjems
- Splicing Factor ChIP and ChRIP: Detection of Splicing and Splicing Factors at Genes by Chromatin Immunoprecipitation / Aparna K Sapra, Fernando Carrillo Oesterreich, Marta Pabis, Imke Listerman, Nicole Bardehle, Karla M Neugebauer
- Yeast Genetics to Investigate the Function of Core Pre-mRNA Splicing Factors / Raymond T O Keefe, Jean D Beggs
- Analysis of HIV-1 RNA Splicing / Simon Duffy, Alan Cochrane
- Analysis of Plant Intron Splicing / Craig G Simpson, Michele Liney, Diane Davidson, Dominika Lewandowska, Maria Kalyna, Sean Chapman, Andrea Barta, John W S Brown
- Modification State-Specific Antibodies / Jordan B Fishman, Olga Kelemen, Eric A Berg
- Analysis of Alternative Splicing in Genetic Mosaics / Shihuang Su, Diana O'Day, Shanzhi Wang, William Mattox
- Manipulation of Splicing Events. Antisense Derivatives of U7 Small Nuclear RNA as Modulators of Pre-mRNA Splicing / Kathrin Meyer, Daniel Schumperli
- Screening for Alternative Splicing Modulators / Peter Stoilov
- Use of Oligonucleotides to Change Splicing / Annemieke Aartsma-Rus, Judith C T van Deutekom
- Changing Signals to the Spliceosome / Denise R Cooper, Niketa A Patel
- Bioinformatic Analysis of Splicing. Overview of Splicing Relevant Databases / Pierre de la Grange
- Analysis of RNA Transcripts by High-Throughput RNA Sequencing / Paolo Ribeca, Vincent Lacroix, Michael Sammeth, Roderic Guig̤
- Identification of Splicing Factor Target Genes by High-Throughput Sequencing / Chaolin Zhang, Michael Q Zhang
- Bioinformatic Analysis of Splicing Events / Zhaiyi Zhang, Stefan Stamm
- Analysis of Pre-mRNA Secondary Structures and Alternative Splicing / Michael Hiller
- Structure Prediction for Alternatively Spliced Proteins / Lukasz Kozlowski, Jerzy Orlowski, Janusz M Bujnicki
- Comparative Genomics Methods for the Prediction of Small RNA-Binding Sites / Rym Kachouri-Lafond, Mihaela Zavolan
- Appendix A1: Yeast Nomenclature Systematic Open Reading Frame (ORF) and Other Genetic Designations
- Appendix A2: Glossary
- Index.