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Design of a single well bioreactor-incubator system with sterile nutrient media supply /

HauptbeschreibungPerfused bioreactors have been developed with different designs and with various cell lines such as fibroblasts, osteoblasts and C2C12 myoblasts. All these current systems are custom made and mostly capable for only one cell type. Small seeding spaces and chambers inhibit longer cel...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Kohlmann, Manuel
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Hamburg : Diplomica Verlag, 2011.
Temas:
Acceso en línea:Texto completo

MARC

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049 |a UAMI 
100 1 |a Kohlmann, Manuel. 
245 1 0 |a Design of a single well bioreactor-incubator system with sterile nutrient media supply /  |c Manuel Kohlmann. 
260 |a Hamburg :  |b Diplomica Verlag,  |c 2011. 
300 |a 1 online resource (98, 2 pages) :  |b illustrations 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
347 |a data file  |2 rda 
500 |a Cover title. 
504 |a Includes bibliographical references. 
520 |a HauptbeschreibungPerfused bioreactors have been developed with different designs and with various cell lines such as fibroblasts, osteoblasts and C2C12 myoblasts. All these current systems are custom made and mostly capable for only one cell type. Small seeding spaces and chambers inhibit longer cell studies and the observation of cell interaction. This study presents an advanced perfusion bioreactor which allows cell observation for longer than 5 days on an area of 2.8cm2 with a volume of 17ml. Initial experiments investigated homogeneity of the heat distribution, which is precisely controlle. 
505 0 |a Design of a Single Well Bioreactor-Incubator System with Sterile Nutrient Media Supply; Acknowledgements; Table of Contens; 1 Introduction; 2 Backround; 2.1 Biological Backround; 2.2 Technical Backround; 3. Methods; 3.1 Biological Strategy from Planting to Formation of 3-D Constructs; 3.2 Bioreactor System Prototype; 3.3 Cell Experimente in the Bioreactor Chamber; 4 Discussion & Conclusion; 5 Future Work; 6 Bibliography; 7 List of used Programs and Versions; 8 List of Figures; 9 Appendix; 9.1 Bioreactor System -- User Manual; 9.2 Flow Chart Explanation; 9.3 C-Code to drive the Stepper Motor. 
505 8 |a 9.4 C-Code to read out the Heatsensors9.5 VB-Code; Lebenslauf von Manuel Kohlmann. 
590 |a ProQuest Ebook Central  |b Ebook Central Academic Complete 
650 0 |a Bioreactors. 
650 0 |a Incubators. 
650 0 |a Culture media (Biology) 
650 2 |a Bioreactors 
650 2 |a Incubators 
650 2 |a Culture Media 
650 6 |a Bioréacteurs. 
650 6 |a Incubateurs (Zootechnie) 
650 6 |a Milieux de culture (Biologie) 
650 7 |a SCIENCE  |x Chemistry  |x Industrial & Technical.  |2 bisacsh 
650 7 |a TECHNOLOGY & ENGINEERING  |x Chemical & Biochemical.  |2 bisacsh 
650 7 |a Bioreactors  |2 fast 
650 7 |a Culture media (Biology)  |2 fast 
650 7 |a Incubators  |2 fast 
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776 0 8 |i Print version:  |a Kohlmann, Manuel.  |t Design of a Single Well Bioreactor-Incubator System with Sterile Nutrient Media Supply.  |d Hamburg : Diplomica Verlag, ©2011  |z 9783842858947 
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