Cargando…
High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins /
The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
River Edge, N.J. :
World Scientific,
2010.
|
| Colección: | Methods in chromatography ;
vol. 2. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Ch. 1. Structure, distribution, biosynthesis and function. 1.1. Introduction. 1.2. Nomenclature. 1.3. Biosynthesis of porphyrins, haem and chlorophyll. 1.4. Biosynthesis of bilins in animals and plants. 1.5. Function of porphyrins and other tetrapyrroles
- ch. 2. High-performance liquid chromatography of porphyrins. 2.1. Introduction. 2.2. HPLC of porphyrin methyl esters. 2.3. HPLC of porphyrin free acids. 2.4. HPLC of porphyrinogens
- ch. 3. Mass spectrometry of porphyrins. 3.1. Introduction. 3.2. Fast Atom Bombardment (FAB) mass spectrometry of porphyrins. 3.3. Laser Desorption/Ionisation Time-of-Flight (LDI-TOF) mass spectrometry of porphyrins. 3.4. Electrospray Ionisation Mass Spectrometry (ESI-MS) and HPLC/ESI-MS of porphyrins
- ch. 4. Porphyrin profiles in blood, urine and faeces by HPLC and HPLC/ESI-MS. 4.1. Introduction. 4.2. Sample collection and preparation. 4.3. Porphyrin excretion patterns and enzyme assays in the porphyrias
- ch. 5. Isolation and characterisation of protoporphyrin glycoconjugates from harderian glands of rodents by HPLC and HPLC/ESI-MS. 5.1. Introduction. 5.2. Extraction of porphyrins from harderian glands. 5.3. HPLC separation of porphyrins from rat harderian gland extract. 5.4. Identification of protoporphyrin glycoconjugates from rat harderian glands by capillary electrophoresis and HPLC/ESI-MS. 5.5. Origin and function of protoporphyrin glycoconjugates in harderian glands of rodents
- ch. 6. HPLC and HPLC/MS of chlorophyll and related compounds. 6.1. Introduction. 6.2. Reversed-phase HPLC of chlorophyll and related compounds in plant extracts. 6.3. Normal-phase HPLC of chlorophyll and related compounds. 6.4. HPLC separation of fluorescent and non-fluorescent chlorophyll catabolites. 6.5. HPLC/MS and MS/MS of chlorophyll and related compounds
- ch. 7. HPLC and HPLC/MS of bilins of animal and plant origin. 7.1. Introduction. 7.2. HPLC separation of bilirubin III[symbol], IX[symbol] and XIII[symbol] isomers. 7.3. HPLC separation of bilirubin conjugates. 7.4. HPLC of biliverdins. 7.5. HPLC of biliviolins. 7.6. HPLC of urobilinoids. 7.7. Analysis and determination of bile pigments in biological and clinical samples by HPLC. 7.8. HPLC/ESI-MS and MS/MS of bile pigments. 7.9. Characterisation of tetrapyrrole pigments in avian eggshells. 7.10. HPLC and HPLC/MS of phycobilins. 7.11. HPLC separation of phycobiliproteins
- ch. 8. Future directions of HPLC and mass spectrometry of tetrapyrroles.