Cargando…
High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins /
The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
River Edge, N.J. :
World Scientific,
2010.
|
| Colección: | Methods in chromatography ;
vol. 2. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
| LEADER | 00000cam a2200000 a 4500 | ||
|---|---|---|---|
| 001 | EBOOKCENTRAL_ocn671655110 | ||
| 003 | OCoLC | ||
| 005 | 20240329122006.0 | ||
| 006 | m o d | ||
| 007 | cr cnu---unuuu | ||
| 008 | 101025s2010 njua ob 001 0 eng d | ||
| 040 | |a N$T |b eng |e pn |c N$T |d E7B |d OCLCQ |d FVL |d OCLCQ |d UIU |d OCLCO |d OCLCQ |d YDXCP |d OCLCF |d NLGGC |d OCLCQ |d AZK |d LOA |d JBG |d COCUF |d AGLDB |d MOR |d CCO |d PIFAG |d OCLCQ |d U3W |d STF |d WRM |d OCLCQ |d VTS |d INT |d VT2 |d AU@ |d OCLCQ |d WYU |d OCLCQ |d M8D |d UKAHL |d ZCU |d UKCRE |d OCLCQ |d OCLCO |d OCLCQ |d OCLCO |d OCLCL | ||
| 019 | |a 712994997 |a 719420412 |a 743436963 |a 748608490 |a 961511276 |a 962633035 |a 966200314 |a 988449380 |a 991922550 |a 1037795302 |a 1038671034 |a 1055384541 |a 1064139642 |a 1081269608 |a 1153535157 |a 1228618477 | ||
| 020 | |a 9789814277198 |q (electronic bk.) | ||
| 020 | |a 9814277193 |q (electronic bk.) | ||
| 020 | |z 9789810230685 | ||
| 020 | |z 9810230680 | ||
| 029 | 1 | |a AU@ |b 000051370046 | |
| 029 | 1 | |a DEBBG |b BV043096753 | |
| 029 | 1 | |a DEBSZ |b 421676655 | |
| 029 | 1 | |a NZ1 |b 14243315 | |
| 035 | |a (OCoLC)671655110 |z (OCoLC)712994997 |z (OCoLC)719420412 |z (OCoLC)743436963 |z (OCoLC)748608490 |z (OCoLC)961511276 |z (OCoLC)962633035 |z (OCoLC)966200314 |z (OCoLC)988449380 |z (OCoLC)991922550 |z (OCoLC)1037795302 |z (OCoLC)1038671034 |z (OCoLC)1055384541 |z (OCoLC)1064139642 |z (OCoLC)1081269608 |z (OCoLC)1153535157 |z (OCoLC)1228618477 | ||
| 050 | 4 | |a QP671.P6 |b L56 2010eb | |
| 072 | 7 | |a SCI |x 013040 |2 bisacsh | |
| 082 | 0 | 4 | |a 547/.593 |2 22 |
| 049 | |a UAMI | ||
| 100 | 1 | |a Lim, Chang Kee. | |
| 245 | 1 | 0 | |a High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins / |c Chang Kee Lim. |
| 260 | |a River Edge, N.J. : |b World Scientific, |c 2010. | ||
| 300 | |a 1 online resource (xii, 232 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 490 | 1 | |a Methods in chromatography ; |v vol. 2 | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Ch. 1. Structure, distribution, biosynthesis and function. 1.1. Introduction. 1.2. Nomenclature. 1.3. Biosynthesis of porphyrins, haem and chlorophyll. 1.4. Biosynthesis of bilins in animals and plants. 1.5. Function of porphyrins and other tetrapyrroles -- ch. 2. High-performance liquid chromatography of porphyrins. 2.1. Introduction. 2.2. HPLC of porphyrin methyl esters. 2.3. HPLC of porphyrin free acids. 2.4. HPLC of porphyrinogens -- ch. 3. Mass spectrometry of porphyrins. 3.1. Introduction. 3.2. Fast Atom Bombardment (FAB) mass spectrometry of porphyrins. 3.3. Laser Desorption/Ionisation Time-of-Flight (LDI-TOF) mass spectrometry of porphyrins. 3.4. Electrospray Ionisation Mass Spectrometry (ESI-MS) and HPLC/ESI-MS of porphyrins -- ch. 4. Porphyrin profiles in blood, urine and faeces by HPLC and HPLC/ESI-MS. 4.1. Introduction. 4.2. Sample collection and preparation. 4.3. Porphyrin excretion patterns and enzyme assays in the porphyrias -- ch. 5. Isolation and characterisation of protoporphyrin glycoconjugates from harderian glands of rodents by HPLC and HPLC/ESI-MS. 5.1. Introduction. 5.2. Extraction of porphyrins from harderian glands. 5.3. HPLC separation of porphyrins from rat harderian gland extract. 5.4. Identification of protoporphyrin glycoconjugates from rat harderian glands by capillary electrophoresis and HPLC/ESI-MS. 5.5. Origin and function of protoporphyrin glycoconjugates in harderian glands of rodents -- ch. 6. HPLC and HPLC/MS of chlorophyll and related compounds. 6.1. Introduction. 6.2. Reversed-phase HPLC of chlorophyll and related compounds in plant extracts. 6.3. Normal-phase HPLC of chlorophyll and related compounds. 6.4. HPLC separation of fluorescent and non-fluorescent chlorophyll catabolites. 6.5. HPLC/MS and MS/MS of chlorophyll and related compounds -- ch. 7. HPLC and HPLC/MS of bilins of animal and plant origin. 7.1. Introduction. 7.2. HPLC separation of bilirubin III[symbol], IX[symbol] and XIII[symbol] isomers. 7.3. HPLC separation of bilirubin conjugates. 7.4. HPLC of biliverdins. 7.5. HPLC of biliviolins. 7.6. HPLC of urobilinoids. 7.7. Analysis and determination of bile pigments in biological and clinical samples by HPLC. 7.8. HPLC/ESI-MS and MS/MS of bile pigments. 7.9. Characterisation of tetrapyrrole pigments in avian eggshells. 7.10. HPLC and HPLC/MS of phycobilins. 7.11. HPLC separation of phycobiliproteins -- ch. 8. Future directions of HPLC and mass spectrometry of tetrapyrroles. | |
| 520 | |a The porphyrins, chlorophylls, bilins and related tetrapyrroles are vital for all living organisms. Natural and synthetic tetrapyrroles are used extensively in foods, cosmetics, biotechnology, pharmaceuticals, diagnostics and medicine. Methods for their separation and characterization therefore, have a very wide area of applications. Yet, there is a dearth of books dedicated to HPLC and HPLC/MS of tetrapyrroles. Lim addresses this problem admirably by providing practical HPLC and HPLC/MS protocols coupled with in-depth chromatographic and mass spectrometric reference data. These are invaluable in the analysis, identification and characterization of porphyrins, chlorophylls, bilins and other related compounds found in biological and clinical materials. HPLC method development and optimization for coupling to mass spectrometry are also described in rich detail. Sample preparation, and suggestions for avoiding procedural artifacts during extraction of clinical and biological samples are discussed. Clinical biochemists involved in biochemical diagnosis of human porphyrias will find this monograph assuredly helpful, as would analysts, biochemists and chemists involved in the separation, isolation and characterization of natural and synthetic tetrapyrroles. Undoubtedly, Lim has contributed a master-piece containing sufficient background material for beginners and up-to-date references for all researchers in the field. | ||
| 590 | |a ProQuest Ebook Central |b Ebook Central Academic Complete | ||
| 590 | |a eBooks on EBSCOhost |b EBSCO eBook Subscription Academic Collection - Worldwide | ||
| 650 | 0 | |a Porphyrins. | |
| 650 | 0 | |a Chlorophyll. | |
| 650 | 0 | |a Tetrapyrroles. | |
| 650 | 0 | |a High performance liquid chromatography. | |
| 650 | 0 | |a Mass spectrometry. | |
| 650 | 6 | |a Porphyrines. | |
| 650 | 6 | |a Chlorophylle. | |
| 650 | 6 | |a Tétrapyrroles. | |
| 650 | 6 | |a Chromatographie en phase liquide à hautes performances. | |
| 650 | 6 | |a Spectrométrie de masse. | |
| 650 | 7 | |a chlorophyll. |2 aat | |
| 650 | 7 | |a mass spectrometry. |2 aat | |
| 650 | 7 | |a SCIENCE |x Chemistry |x Organic. |2 bisacsh | |
| 650 | 7 | |a Chlorophyll |2 fast | |
| 650 | 7 | |a High performance liquid chromatography |2 fast | |
| 650 | 7 | |a Mass spectrometry |2 fast | |
| 650 | 7 | |a Porphyrins |2 fast | |
| 650 | 7 | |a Tetrapyrroles |2 fast | |
| 758 | |i has work: |a High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins (Text) |1 https://id.oclc.org/worldcat/entity/E39PCFC9XPT8FkhkvpCBjbtyq3 |4 https://id.oclc.org/worldcat/ontology/hasWork | ||
| 776 | 0 | 8 | |i Print version: |a Lim, Chang Kee. |t High-performance liquid chromatography and mass spectrometry of porphyrins, chlorophylls and bilins. |d River Edge, N.J. : World Scientific, 2010 |z 9789810230685 |w (DLC) 2010275008 |w (OCoLC)643764043 |
| 830 | 0 | |a Methods in chromatography ; |v vol. 2. | |
| 856 | 4 | 0 | |u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=1679396 |z Texto completo |
| 938 | |a Askews and Holts Library Services |b ASKH |n AH24686363 | ||
| 938 | |a ebrary |b EBRY |n ebr10421998 | ||
| 938 | |a EBSCOhost |b EBSC |n 340495 | ||
| 938 | |a YBP Library Services |b YANK |n 3511436 | ||
| 994 | |a 92 |b IZTAP | ||