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Biotechnology : a Laboratory Course.
The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with ba...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Burlington :
Elsevier,
1996.
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| Edición: | 2nd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
| LEADER | 00000cam a2200000Mi 4500 | ||
|---|---|---|---|
| 001 | EBOOKCENTRAL_ocn476132220 | ||
| 003 | OCoLC | ||
| 005 | 20240329122006.0 | ||
| 006 | m o d | ||
| 007 | cr mn|---||||| | ||
| 008 | 091207s1996 vtu o 000 0 eng d | ||
| 040 | |a EBLCP |b eng |e pn |c EBLCP |d OCLCQ |d ZCU |d MERUC |d ICG |d OCLCO |d OCLCF |d OCLCQ |d OCLCO |d OCLCQ |d DKC |d AU@ |d OCLCQ |d HS0 |d OCL |d OCLCQ |d OCLCO |d OCLCQ |d OCLCO | ||
| 020 | |a 9780080528199 |q (electronic bk.) | ||
| 020 | |a 0080528198 |q (electronic bk.) | ||
| 029 | 1 | |a DEBBG |b BV044049493 | |
| 035 | |a (OCoLC)476132220 | ||
| 050 | 4 | |a TP248.2 .B374 1996 |a TP248.2.B3 |a TP248.2.B374 1996 | |
| 082 | 0 | 4 | |a 660.6078 |a 660/.6/078 20 |a 660.6 |
| 049 | |a UAMI | ||
| 100 | 1 | |a Becker, Jeffrey M. | |
| 245 | 1 | 0 | |a Biotechnology : |b a Laboratory Course. |
| 250 | |a 2nd ed. | ||
| 260 | |a Burlington : |b Elsevier, |c 1996. | ||
| 300 | |a 1 online resource (283 pages) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 520 | |a The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles demonstrated are. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Biotechnology: A Laboratory Course; Copyright Page; Contents; Preface to the Second Edition; Preface to the First Edition; Acknowledgments; Suggested Schedule for Exercises; Introductory Notes: Record Keeping and Safety Rules; Format of Student Laboratory Records; The Ten Commandments of Record Keeping; Safety Rules in the Laboratory; Exercise 1. Aseptic Technique and Establishing Pure Cultures: The Streak Plate and Culture Transfer; Exercise 2. Preparation of Culture Media; Exercise 3. The Growth Curve; Exercise 4. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep | |
| 505 | 8 | |a Exercise 5. Purification, Concentration, and Quantitation of DNAExercise 6. Large-Scale Isolation of Plasmid DNA by Column Chromatography; Exercise 7. Amplification of a lacZ Gene Fragment by the Polymerase Chain Reaction; Exercise 8. Restriction Digestion and Agarose Gel Electrophoresis; Exercise 9. Southern Transfer; Exercise 10. Preparation, Purification, and Hybridization of Probe; Exercise 11. Transformation of Saccharomyces cerevisiae; Exercise 12. Isolation of Plasmid from Yeast and Escherichia coil Transformation; Exercise 13. Protein Assays | |
| 505 | 8 | |a Exercise 14. Qualitative Assay for b-Galactosidase in Yeast ColoniesExercise 15. Determination of b-Galactosidase in Permeabilized Yeast Cells; Exercise 16. Assay of b-Galactosidase in Cell Extracts; Exercise 17. b-Galactosidase Purification; Exercise 18. Western Blot: Probe of Protein Blot with Antibody to b-Galactosidase; Appendix 1. Alternative Protocols and Experiments; Exercise 1A Isolation and Characterization of Auxotrophic Yeast Mutants; Exercise 2A Measurement of pH; Exercise 3A Use of the Spectrophotometer; Exercise 6A Isolation of Plasmid DNA: The Maxi-Prep | |
| 505 | 8 | |a Exercise 10A Colony HybridizationAppendix 2. Buffer Solutions; Appendix 3. Preparation of Buffers and Solutions; Appendix 4. Properties of Some Common Concentrated Acids and Bases; Appendix 5. Use of Micropipettors; Appendix 6. Safe Handling of Microorganisms; Appendix 7. List of Cultures; Appendix 8. Storage of Cultures and DNA; Appendix 9. Sterilization Methods; Appendix 10. Preparation of Stock Solutions for Culture Media; Appendix 11. Growth in Liquid Medium; Appendix 12. Determination of Viable Cells; Appendix 13. Determination of Cell Mass; Appendix 14. Determination of Cell Number | |
| 505 | 8 | |a Appendix 15. Nomenclature of StrainsAppendix 16. Glassware and Plasticware; Appendix 17. Preparation of Tris and EDTA; Appendix 18. Basic Rules for Handling Enzymes; Appendix 19. Effects of Common Contaminants on Protein Assays; Appendix 20. Manufacturers' and Distributors' Addresses; Appendix 21. Surfing the Bionet: World Wide Web Addresses; Glossary; Index | |
| 590 | |a ProQuest Ebook Central |b Ebook Central Academic Complete | ||
| 650 | 0 | |a Biochemical engineering. | |
| 650 | 0 | |a Biotechnology |v Laboratory manuals. | |
| 650 | 6 | |a Génie biochimique. | |
| 650 | 6 | |a Biotechnologie |v Manuels de laboratoire. | |
| 650 | 7 | |a Biochemical engineering |2 fast | |
| 650 | 7 | |a Biotechnology |2 fast | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
| 700 | 1 | |a Caldwell, Guy A. | |
| 700 | 1 | |a Zachgo, Eve Ann. | |
| 776 | 1 | |z 9780120845620 | |
| 856 | 4 | 0 | |u https://ebookcentral.uam.elogim.com/lib/uam-ebooks/detail.action?docID=331913 |z Texto completo |
| 938 | |a ProQuest Ebook Central |b EBLB |n EBL331913 | ||
| 994 | |a 92 |b IZTAP | ||